Gene description for LAMTOR2
Gene name late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
Gene symbol LAMTOR2
Other names/aliases ENDAP
HSPC003
MAPBPIP
MAPKSP1AP
ROBLD3
Ragulator2
p14
Species Homo sapiens
 Database cross references - LAMTOR2
ExoCarta ExoCarta_28956
Vesiclepedia VP_28956
Entrez Gene 28956
HGNC 29796
MIM 610389
UniProt Q9Y2Q5  
 LAMTOR2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for LAMTOR2
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    molecular adaptor activity GO:0060090 IDA
Biological Process
    regulation of cell growth GO:0001558 IMP
    protein localization GO:0008104 IMP
    fibroblast migration GO:0010761 IEA
    positive regulation of TOR signaling GO:0032008 IBA
    positive regulation of TOR signaling GO:0032008 IMP
    TORC1 signaling GO:0038202 NAS
    positive regulation of MAPK cascade GO:0043410 IEA
    cellular response to amino acid stimulus GO:0071230 IBA
    cellular response to amino acid stimulus GO:0071230 IDA
    cellular response to amino acid stimulus GO:0071230 IMP
    regulation of cell-substrate junction organization GO:0150116 IMP
    protein localization to cell junction GO:1902414 IEA
    positive regulation of TORC1 signaling GO:1904263 IDA
Subcellular Localization
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    late endosome GO:0005770 ISS
    plasma membrane GO:0005886 TAS
    endosome membrane GO:0010008 TAS
    late endosome membrane GO:0031902 NAS
    specific granule membrane GO:0035579 TAS
    tertiary granule membrane GO:0070821 TAS
    Ragulator complex GO:0071986 IBA
    Ragulator complex GO:0071986 IDA
    Ragulator complex GO:0071986 IPI
    FNIP-folliculin RagC/D GAP GO:1990877 IDA
 Experiment description of studies that identified LAMTOR2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for LAMTOR2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NDUFV1 4723
Co-fractionation Homo sapiens
2 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 AK4 205
Co-fractionation Homo sapiens
4 ANLN 54443
Affinity Capture-MS Homo sapiens
5 MTHFD2 10797
Co-fractionation Homo sapiens
6 CSNK1G3 1456
Affinity Capture-MS Homo sapiens
7 LAMTOR4 389541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 POR 5447
Co-fractionation Homo sapiens
9 VAMP3 9341
Affinity Capture-MS Homo sapiens
10 LOH12CR1 118426
Affinity Capture-MS Homo sapiens
11 NDUFS1 4719
Co-fractionation Homo sapiens
12 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
13 ILF3 3609
Co-fractionation Homo sapiens
14 STX12 23673
Affinity Capture-MS Homo sapiens
15 BLOC1S2 282991
Affinity Capture-MS Homo sapiens
16 ATP6V1A 523
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
17 LAMTOR1 55004
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
18 RRAGA 10670
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 LRRC59 55379
Co-fractionation Homo sapiens
20 NDUFA9 4704
Co-fractionation Homo sapiens
21 ATP6V1G2-DDX39B  
Affinity Capture-MS Homo sapiens
22 TPP1 1200
Co-fractionation Homo sapiens
23 NNT 23530
Co-fractionation Homo sapiens
24 LONP1 9361
Co-fractionation Homo sapiens
25 UQCRC2 7385
Co-fractionation Homo sapiens
26 RAB2A 5862
Affinity Capture-MS Homo sapiens
27 RING1 6015
Affinity Capture-MS Homo sapiens
28 NUFIP2 57532
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 PDHA1 5160
Co-fractionation Homo sapiens
30 P4HA1 5033
Co-fractionation Homo sapiens
31 RPTOR 57521
Affinity Capture-Western Homo sapiens
32 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
33 RRAGD 58528
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
34 BTF3 689
Affinity Capture-MS Homo sapiens
35 CDC23 8697
Affinity Capture-MS Homo sapiens
36 SLC18B1 116843
Affinity Capture-MS Homo sapiens
37 TXNDC12 51060
Co-fractionation Homo sapiens
38 SCAMP3 10067
Affinity Capture-MS Homo sapiens
39 PEBP1 5037
Co-fractionation Homo sapiens
40 FTH1 2495
Affinity Capture-MS Homo sapiens
41 TMEM55B 90809
Affinity Capture-MS Homo sapiens
42 ARL8B 55207
Affinity Capture-MS Homo sapiens
43 RRAGB 10325
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
44 ECE1 1889
Co-fractionation Homo sapiens
45 MAPK1 5594
Co-fractionation Homo sapiens
46 SLC38A9  
Affinity Capture-MS Homo sapiens
47 HCCS 3052
Co-fractionation Homo sapiens
48 LRPPRC 10128
Co-fractionation Homo sapiens
49 C10orf32  
Affinity Capture-MS Homo sapiens
50 FASN 2194
Positive Genetic Homo sapiens
51 VRK1 7443
Affinity Capture-MS Homo sapiens
52 BLOC1S1  
Affinity Capture-MS Homo sapiens
53 CDC16 8881
Affinity Capture-MS Homo sapiens
54 NDUFS3 4722
Co-fractionation Homo sapiens
55 ABHD11 83451
Co-fractionation Homo sapiens
56 TMEM184C  
Affinity Capture-MS Homo sapiens
57 FLOT1 10211
Co-fractionation Homo sapiens
58 SDHB 6390
Co-fractionation Homo sapiens
59 MTOR 2475
Affinity Capture-Western Homo sapiens
60 TUBD1  
Affinity Capture-MS Homo sapiens
61 DYNLRB1 83658
Co-fractionation Homo sapiens
62 ATP6V1B2 526
Co-fractionation Homo sapiens
63 LAMP1 3916
Affinity Capture-MS Homo sapiens
64 YES1 7525
Affinity Capture-MS Homo sapiens
65 RAB7A 7879
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 RAB14 51552
Affinity Capture-MS Homo sapiens
67 MRPS21  
Co-fractionation Homo sapiens
68 UQCRC1 7384
Co-fractionation Homo sapiens
69 ATP5C1 509
Co-fractionation Homo sapiens
70 ABCB10 23456
Co-fractionation Homo sapiens
71 WHSC1L1  
Affinity Capture-MS Homo sapiens
72 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
73 STYX  
Affinity Capture-MS Homo sapiens
74 TRIAP1  
Co-fractionation Homo sapiens
75 LYPLA1 10434
Co-fractionation Homo sapiens
76 CPNE8 144402
Co-fractionation Homo sapiens
77 MSRB2  
Co-fractionation Homo sapiens
78 LAMTOR3 8649
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
79 RRAGC 64121
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
80 C17orf59 54785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
81 MAP2K1 5604
Affinity Capture-Western Homo sapiens
82 TMEM192 201931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 CDC27 996
Affinity Capture-MS Homo sapiens
84 TMEM185A  
Affinity Capture-MS Homo sapiens
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