Gene description for RRAGB
Gene name Ras-related GTP binding B
Gene symbol RRAGB
Other names/aliases RAGB
bA465E19.1
Species Homo sapiens
 Database cross references - RRAGB
ExoCarta ExoCarta_10325
Vesiclepedia VP_10325
Entrez Gene 10325
HGNC 19901
MIM 300725
UniProt Q5VZM2  
 RRAGB identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for RRAGB
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IMP
    guanyl ribonucleotide binding GO:0032561 IDA
    GTPase binding GO:0051020 IPI
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    protein localization GO:0008104 IMP
    cellular response to starvation GO:0009267 IBA
    negative regulation of autophagy GO:0010507 IBA
    regulation of TOR signaling GO:0032006 IGI
    positive regulation of TOR signaling GO:0032008 IMP
    cellular response to amino acid starvation GO:0034198 IGI
    cellular response to amino acid stimulus GO:0071230 IMP
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 IMP
    cellular response to leucine starvation GO:1990253 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IBA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    cytosol GO:0005829 TAS
    Gtr1-Gtr2 GTPase complex GO:1990131 IBA
 Experiment description of studies that identified RRAGB in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RRAGB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDH13 1012
Affinity Capture-MS Homo sapiens
2 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MIB1 57534
Affinity Capture-MS Homo sapiens
4 CD164L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 LAMTOR4 389541
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 LGALS9 3965
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 FLCN 201163
Affinity Capture-Western Homo sapiens
8 GPR17 2840
Affinity Capture-MS Homo sapiens
9 LAMTOR1 55004
Co-localization Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 GSS 2937
Co-fractionation Homo sapiens
11 TSC2 7249
Affinity Capture-Western Homo sapiens
12 LGALS9C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 RALBP1 10928
Affinity Capture-MS Homo sapiens
14 SQSTM1 8878
Affinity Capture-Western Homo sapiens
15 RASAL1 8437
Co-fractionation Homo sapiens
16 LGALS3 3958
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 SPG21 51324
Affinity Capture-MS Homo sapiens
18 DEPDC5  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 RPTOR 57521
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
20 YWHAB 7529
Co-fractionation Homo sapiens
21 RRAGD 58528
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
22 LAMTOR2 28956
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PDHB 5162
Co-fractionation Homo sapiens
24 RABGGTB 5876
Affinity Capture-MS Homo sapiens
25 TMOD1  
Affinity Capture-MS Homo sapiens
26 LGALS9B 284194
Affinity Capture-MS Homo sapiens
27 CSE1L 1434
Co-fractionation Homo sapiens
28 CKB 1152
Co-fractionation Homo sapiens
29 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
30 WDR24  
Affinity Capture-Western Homo sapiens
31 NUDT1  
Affinity Capture-MS Homo sapiens
32 BLVRA 644
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CHODL 140578
Affinity Capture-MS Homo sapiens
34 SSUH2  
Affinity Capture-MS Homo sapiens
35 C9orf72  
Affinity Capture-MS Homo sapiens
36 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CCR6  
Affinity Capture-MS Homo sapiens
38 LGALS8 3964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
40 LAMTOR3 8649
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 APP 351
Reconstituted Complex Homo sapiens
43 RRAGC 64121
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
44 MTOR 2475
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
45 TRIM16 10626
Affinity Capture-Western Homo sapiens
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