Gene description for TSC2
Gene name tuberous sclerosis 2
Gene symbol TSC2
Other names/aliases LAM
PPP1R160
TSC4
Species Homo sapiens
 Database cross references - TSC2
ExoCarta ExoCarta_7249
Vesiclepedia VP_7249
Entrez Gene 7249
HGNC 12363
MIM 191092
UniProt P49815  
 TSC2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for TSC2
Molecular Function
    14-3-3 protein binding GO:0071889 IEA
    GTPase activator activity GO:0005096 IDA
    protein heterodimerization activity GO:0046982 IEA
    protein homodimerization activity GO:0042803 IPI
    phosphatase binding GO:0019902 IDA
    protein binding GO:0005515 IPI
Biological Process
    protein localization GO:0008104 ISS
    protein transport into plasma membrane raft GO:0044861 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    negative regulation of Wnt signaling pathway GO:0030178 IBA
    protein kinase B signaling GO:0043491 ISS
    viral process GO:0016032 IEA
    neural tube closure GO:0001843 ISS
    negative regulation of cell size GO:0045792 IEA
    negative regulation of protein kinase activity GO:0006469 ISS
    negative regulation of MAP kinase activity GO:0043407 IEA
    protein heterooligomerization GO:0051291 IEA
    negative regulation of TOR signaling GO:0032007 IBA
    heart development GO:0007507 ISS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    cell cycle arrest GO:0007050 TAS
    insulin-like growth factor receptor signaling pathway GO:0048009 ISS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of GTPase activity GO:0043547 IBA
    negative regulation of insulin receptor signaling pathway GO:0046627 IBA
    gene expression GO:0010467 TAS
    endocytosis GO:0006897 TAS
    response to hypoxia GO:0001666 IEA
    negative regulation of protein kinase B signaling GO:0051898 ISS
    protein import into nucleus GO:0006606 ISS
    cell projection organization GO:0030030 IEA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    regulation of endocytosis GO:0030100 ISS
    negative regulation of phosphatidylinositol 3-kinase signaling GO:0014067 ISS
    regulation of insulin receptor signaling pathway GO:0046626 ISS
    innate immune response GO:0045087 TAS
    positive chemotaxis GO:0050918 ISS
    negative regulation of cell proliferation GO:0008285 ISS
    phosphatidylinositol 3-kinase signaling GO:0014065 IEA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    glucose import GO:0046323 IEA
    vesicle-mediated transport GO:0016192 TAS
    regulation of cell cycle GO:0051726 IBA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    acute-phase response GO:0006953 IEA
    protein homooligomerization GO:0051260 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
Subcellular Localization
    TSC1-TSC2 complex GO:0033596 IDA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    cytoskeleton GO:0005856 IEA
    neuronal cell body GO:0043025 IEA
    growth cone GO:0030426 IEA
    caveola GO:0005901 IEA
    Golgi apparatus GO:0005794 IDA
    membrane GO:0016020 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    dendrite GO:0030425 IEA
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified TSC2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 193
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|CD9
EV Enriched markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 194
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 195
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TSC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRKAA1 5562
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 CCND2  
Affinity Capture-Western Homo sapiens
3 GSK3B 2932
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 CDKN1B 1027
Affinity Capture-Western Homo sapiens
5 MAPK1 5594
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 RAB5A 5868
Co-purification Homo sapiens
7 PAM 5066
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Two-hybrid Homo sapiens
8 YWHAA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
9 SKP2  
Phenotypic Suppression Homo sapiens
10 UBE3A 7337
Reconstituted Complex Homo sapiens
11 PTK2 5747
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 RALA 5898
Affinity Capture-Western Homo sapiens
13 AXIN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
14 RHEB 6009
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
15 FOXO1A  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
16 YWHAZ 7534
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
17 RAP1A 5906
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
18 PLK1 5347
Affinity Capture-Western Homo sapiens
19 YWHAQ 10971
Affinity Capture-MS Homo sapiens
20 TP53  
Synthetic Rescue Mus musculus
21 AKT1 207
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 CCND1 595
Affinity Capture-Western Homo sapiens
23 TSC1 7248
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 CAV1 857
Co-purification Homo sapiens
25 RPS6KA1 6195
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
26 CCND3  
Affinity Capture-Western Homo sapiens
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