Gene description for P4HA3
Gene name prolyl 4-hydroxylase, alpha polypeptide III
Gene symbol P4HA3
Other names/aliases -
Species Homo sapiens
 Database cross references - P4HA3
ExoCarta ExoCarta_283208
Vesiclepedia VP_283208
Entrez Gene 283208
HGNC 30135
MIM 608987
UniProt Q7Z4N8  
 P4HA3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for P4HA3
Molecular Function
    procollagen-proline 4-dioxygenase activity GO:0004656 IBA
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
    L-ascorbic acid binding GO:0031418 IEA
Biological Process
    collagen fibril organization GO:0030199 IBA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum lumen GO:0005788 TAS
 Experiment description of studies that identified P4HA3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for P4HA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SENP3 26168
Affinity Capture-MS Homo sapiens
2 ZNHIT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TSC2 7249
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PSMB4 5692
Two-hybrid Homo sapiens
5 RBM15B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 AES 166
Two-hybrid Homo sapiens
7 ZC3H12A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
9 MCCD1  
Two-hybrid Homo sapiens
10 SIX1  
Affinity Capture-MS Homo sapiens
11 FBRS 64319
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ATN1  
Two-hybrid Homo sapiens
13 WDR62  
Two-hybrid Homo sapiens
14 SRCAP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 VPS72  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CHAF1A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 FAM171A2 284069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CT55  
Two-hybrid Homo sapiens
19 HGS 9146
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
20 SCRIB 23513
Affinity Capture-MS Homo sapiens
21 OIP5  
Two-hybrid Homo sapiens
22 ANAPC11  
Two-hybrid Homo sapiens
23 ACOT8 10005
Two-hybrid Homo sapiens
24 NFATC2IP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PDF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 KRTAP19-5  
Two-hybrid Homo sapiens
27 YAF2  
Affinity Capture-MS Homo sapiens
28 KIF21A 55605
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 CKMT2  
Two-hybrid Homo sapiens
30 ZNF579  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 AGAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MAGEA2  
Two-hybrid Homo sapiens
33 ZNF444  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 BCL2L11  
Affinity Capture-MS Homo sapiens
35 DTNBP1  
Two-hybrid Homo sapiens
36 KRTAP8-1  
Two-hybrid Homo sapiens
37 RSRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 UBR5 51366
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SERTAD1  
Two-hybrid Homo sapiens
40 TIGD5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 GPSM3  
Two-hybrid Homo sapiens
42 PEX14 5195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 MEOX2  
Two-hybrid Homo sapiens
44 P4HB 5034
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 GSG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NICN1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
47 TOX2  
Two-hybrid Homo sapiens
48 FGFR1OP  
Affinity Capture-MS Homo sapiens
49 CERCAM 51148
Two-hybrid Homo sapiens
50 CTAG1A  
Two-hybrid Homo sapiens
51 YEATS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 DISC1 27185
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 C1QTNF2  
Two-hybrid Homo sapiens
54 HSPA5 3309
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 PDIA2 64714
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 WDR33 55339
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SF1 7536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 YLPM1 56252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 NXF1 10482
Affinity Capture-RNA Homo sapiens
60 MAGEC2  
Two-hybrid Homo sapiens
61 PPHLN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ACTR6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 DMAP1 55929
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FXR2 9513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 ZMYND8 23613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SCARA5 286133
Two-hybrid Homo sapiens
67 ZNF687  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 ZBTB7B  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
69 SESTD1 91404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 KRTAP6-1  
Two-hybrid Homo sapiens
71 PATZ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CASKIN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ZNF787  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 BAG4  
Two-hybrid Homo sapiens
75 CCDC8  
Affinity Capture-MS Homo sapiens
76 SATB1 6304
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CLPP 8192
Two-hybrid Homo sapiens
78 NUTM1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
79 GGA3 23163
Two-hybrid Homo sapiens
80 TP53BP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ZNF592 9640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 PROP1  
Two-hybrid Homo sapiens
83 TRPS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 BLOC1S2 282991
Two-hybrid Homo sapiens
85 SRRM1 10250
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 VPS37C 55048
Two-hybrid Homo sapiens
87 KMT2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 ADRA2C  
Two-hybrid Homo sapiens
89 CTAG1B  
Two-hybrid Homo sapiens
90 MAGEA2B  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
91 LRP2BP  
Two-hybrid Homo sapiens
92 FAM120C 54954
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 PWWP2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 NF1 4763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
95 RUSC1  
Two-hybrid Homo sapiens
96 STMN3  
Two-hybrid Homo sapiens
97 CTNNBIP1 56998
Two-hybrid Homo sapiens
98 B9D2 80776
Two-hybrid Homo sapiens
99 SLBP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 MTCL1 23255
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 PWWP2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 ZNF318 24149
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 NDN 4692
Two-hybrid Homo sapiens
104 SERTAD3  
Two-hybrid Homo sapiens
105 MSRA 4482
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which P4HA3 is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes TAS Reactome
Collagen formation TAS Reactome
Extracellular matrix organization TAS Reactome





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