Gene description for CSNK2A1
Gene name casein kinase 2, alpha 1 polypeptide
Gene symbol CSNK2A1
Other names/aliases CK2A1
CKII
CSNK2A3
Species Homo sapiens
 Database cross references - CSNK2A1
ExoCarta ExoCarta_1457
Entrez Gene 1457
HGNC 2457
MIM 115440
UniProt P68400  
 CSNK2A1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Thymus 23844026    
 Gene ontology annotations for CSNK2A1
Molecular Function
    protein N-terminus binding GO:0047485 IPI
    ATP binding GO:0005524 IEA
    Hsp90 protein binding GO:0051879 TAS
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
Biological Process
    positive regulation of Wnt signaling pathway GO:0030177 IMP
    Wnt signaling pathway GO:0016055 IEA
    mitotic cell cycle GO:0000278 TAS
    transcription, DNA-templated GO:0006351 IEA
    positive regulation of cell proliferation GO:0008284 IDA
    signal transduction GO:0007165 TAS
    positive regulation of protein catabolic process GO:0045732 IDA
    rhythmic process GO:0048511 IEA
    positive regulation of cell growth GO:0030307 IDA
    chaperone-mediated protein folding GO:0061077 TAS
    axon guidance GO:0007411 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IMP
    mitotic spindle checkpoint GO:0071174 IMP
    regulation of transcription, DNA-templated GO:0006355 IEA
    protein phosphorylation GO:0006468 IDA
Subcellular Localization
    PcG protein complex GO:0031519 IDA
    NuRD complex GO:0016581 IDA
    Sin3 complex GO:0016580 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified CSNK2A1 in exosomes
1
Experiment ID 282
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 283
ISEV standards
CEM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81|EpCAM
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 255
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 256
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 257
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSNK2A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 DVL1 1855
Affinity Capture-Western Homo sapiens
3 BRF1  
Affinity Capture-Western Homo sapiens
4 SPI1  
Biochemical Activity Homo sapiens
5 CSNK2B 1460
Far Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
6 MAZ 4150
Biochemical Activity Homo sapiens
7 PAFAH1B1 5048
Biochemical Activity Homo sapiens
8 DVL2 1856
Affinity Capture-Western Homo sapiens
9 FGF2 2247
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
10 ZHX1  
Two-hybrid Homo sapiens
11 YWHAB 7529
Affinity Capture-MS Homo sapiens
12 PSMA4 5685
Biochemical Activity Homo sapiens
13 PIN1 5300
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
14 MME 4311
Biochemical Activity Homo sapiens
15 APC  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 HNRNPA2B1 3181
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
17 ARR3 407
Biochemical Activity Homo sapiens
18 KLF1  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
19 CDC25B 994
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
20 RPS6KB1  
Reconstituted Complex Homo sapiens
21 RELA  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
22 XKRY  
Biochemical Activity Homo sapiens
23 CREBBP  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
24 IRF2 3660
Biochemical Activity Homo sapiens
25 HSP90AA1 3320
Reconstituted Complex Homo sapiens
26 ATF1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
Biochemical Activity Homo sapiens
27 RPL5 6125
Biochemical Activity Homo sapiens
28 ATF2  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
29 JUN 3725
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
30 DDIT3 1649
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
31 RNPS1 10921
Affinity Capture-MS Homo sapiens
32 FOS 2353
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
33 SPIB  
Biochemical Activity Homo sapiens
34 HCLS1  
Biochemical Activity Homo sapiens
35 VAMP4  
Biochemical Activity Homo sapiens
36 ARRB1 408
Affinity Capture-MS Homo sapiens
37 PTEN  
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
38 DVL3  
Affinity Capture-Western Homo sapiens
39 EEF1D 1936
Biochemical Activity Homo sapiens
40 TOP1 7150
Biochemical Activity Homo sapiens
41 CHEK1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
42 CKIP-1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
43 HDAC2 3066
Biochemical Activity Homo sapiens
44 UBTF 7343
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
45 XK  
Biochemical Activity Homo sapiens
46 MAPK14 1432
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
47 LYST 1130
Two-hybrid Homo sapiens
48 HSP90B1 7184
Reconstituted Complex Homo sapiens
49 FGF1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
50 BRCA1 672
Reconstituted Complex Homo sapiens
51 MGMT 4255
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which CSNK2A1 is involved
PathwayEvidenceSource
Condensation of Prometaphase Chromosomes TAS Reactome
Signal transduction by L1 TAS Reactome
WNT mediated activation of DVL TAS Reactome





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