Gene description for FGF2
Gene name fibroblast growth factor 2 (basic)
Gene symbol FGF2
Other names/aliases BFGF
FGF-2
FGFB
HBGF-2
Species Homo sapiens
 Database cross references - FGF2
ExoCarta ExoCarta_2247
Entrez Gene 2247
HGNC 3676
MIM 134920
UniProt P09038  
 FGF2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for FGF2
Molecular Function
    cytokine activity GO:0005125 IDA
    growth factor activity GO:0008083 IDA
    protein binding GO:0005515 IPI
    chemoattractant activity GO:0042056 IDA
    heparin binding GO:0008201 IEA
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 IDA
    fibroblast growth factor receptor binding GO:0005104 IPI
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    positive regulation of endothelial cell chemotaxis to fibroblast growth factor GO:2000546 IDA
    positive regulation of sprouting angiogenesis GO:1903672 IDA
    branching involved in ureteric bud morphogenesis GO:0001658 IDA
    somatic stem cell maintenance GO:0035019 TAS
    negative regulation of cell death GO:0060548 IDA
    activation of MAPK activity GO:0000187 TAS
    insulin receptor signaling pathway GO:0008286 TAS
    positive regulation of cell proliferation GO:0008284 IGI
    release of sequestered calcium ion into cytosol GO:0051209 IDA
    signal transduction GO:0007165 NAS
    inositol phosphate biosynthetic process GO:0032958 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of endothelial cell proliferation GO:0001938 IMP
    embryonic morphogenesis GO:0048598 TAS
    positive regulation of MAP kinase activity GO:0043406 IDA
    chondroblast differentiation GO:0060591 IDA
    positive regulation of phospholipase C activity GO:0010863 IDA
    positive regulation of cardiac muscle cell proliferation GO:0060045 IDA
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IDA
    phosphatidylinositol biosynthetic process GO:0006661 IDA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IDA
    chemotaxis GO:0006935 TAS
    negative regulation of fibroblast migration GO:0010764 IDA
    extracellular matrix organization GO:0030198 TAS
    regulation of endothelial cell chemotaxis to fibroblast growth factor GO:2000544 IDA
    innate immune response GO:0045087 TAS
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    wound healing GO:0042060 TAS
    negative regulation of wound healing GO:0061045 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IDA
    regulation of angiogenesis GO:0045765 TAS
    nervous system development GO:0007399 TAS
    hyaluronan catabolic process GO:0030214 IDA
    positive regulation of cell division GO:0051781 IEA
    cell migration involved in sprouting angiogenesis GO:0002042 IGI
    positive regulation of angiogenesis GO:0045766 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    Ras protein signal transduction GO:0007265 TAS
    growth factor dependent regulation of skeletal muscle satellite cell proliferation GO:0014843 IMP
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    organ morphogenesis GO:0009887 TAS
    negative regulation of blood vessel endothelial cell migration GO:0043537 IDA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive chemotaxis GO:0050918 IDA
    positive regulation of cell fate specification GO:0042660 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IEA
    extracellular space GO:0005615 TAS
 Experiment description of studies that identified FGF2 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for FGF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 VTN 7448
Reconstituted Complex Homo sapiens
2 PF4  
Invivo Homo sapiens
Invitro Homo sapiens
3 FGFBP1 9982
Invivo Homo sapiens
4 FGFR4 2264
Invitro Homo sapiens
5 CD44 960
Invivo Homo sapiens
6 CSNK2B 1460
Reconstituted Complex Homo sapiens
7 FGFR2 2263
Invivo Homo sapiens
8 RPS19 6223
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
9 SDC3  
Invitro Homo sapiens
10 GPC4 2239
Invitro Homo sapiens
Invivo Homo sapiens
11 FGFR1 2260
Invivo Homo sapiens
Invitro Homo sapiens
12 CXCL13  
Invitro Homo sapiens
13 CSNK2A1 1457
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
14 SDC2 6383
Co-purification Homo sapiens
15 RPL6 6128
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 CSNK2A2 1459
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
17 SDC4 6385
Co-purification Homo sapiens
18 SDC1 6382
Co-purification Homo sapiens
19 GPC3  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which FGF2 is involved
PathwayEvidenceSource
Activated point mutants of FGFR2 TAS Reactome
Constitutive Signaling by Aberrant PI3K in Cancer TAS Reactome
FGFR1b ligand binding and activation TAS Reactome
FGFR1c ligand binding and activation TAS Reactome
FGFR2b ligand binding and activation TAS Reactome
FGFR2c ligand binding and activation TAS Reactome
FGFR3 mutant receptor activation TAS Reactome
FGFR3c ligand binding and activation TAS Reactome
FGFR4 ligand binding and activation TAS Reactome
FRS-mediated FGFR1 signaling TAS Reactome
FRS-mediated FGFR2 signaling TAS Reactome
FRS-mediated FGFR3 signaling TAS Reactome
FRS2-mediated FGFR4 signaling TAS Reactome
Negative regulation of FGFR1 signaling TAS Reactome
Negative regulation of FGFR2 signaling TAS Reactome
Negative regulation of FGFR3 signaling TAS Reactome
Negative regulation of FGFR4 signaling TAS Reactome
Non-integrin membrane-ECM interactions TAS Reactome
Phospholipase C-mediated cascade: FGFR1 TAS Reactome
Phospholipase C-mediated cascade; FGFR2 TAS Reactome
Phospholipase C-mediated cascade; FGFR3 TAS Reactome
Phospholipase C-mediated cascade; FGFR4 TAS Reactome
PI-3K cascade:FGFR1 TAS Reactome
PI-3K cascade:FGFR2 TAS Reactome
PI-3K cascade:FGFR3 TAS Reactome
PI-3K cascade:FGFR4 TAS Reactome
PI3K Cascade TAS Reactome
PI3K Cascade TAS Reactome
PIP3 activates AKT signaling TAS Reactome
POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation TAS Reactome
SHC-mediated cascade:FGFR1 TAS Reactome
SHC-mediated cascade:FGFR2 TAS Reactome
SHC-mediated cascade:FGFR3 TAS Reactome
SHC-mediated cascade:FGFR4 TAS Reactome
Signaling by activated point mutants of FGFR1 TAS Reactome
Signaling by activated point mutants of FGFR3 TAS Reactome
Signaling by FGFR1 mutants TAS Reactome
Signaling by FGFR2 mutants TAS Reactome
Signaling by FGFR3 mutants TAS Reactome
Signaling by FGFR4 mutants TAS Reactome
Syndecan interactions TAS Reactome





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