Gene description for COMT
Gene name catechol-O-methyltransferase
Gene symbol COMT
Other names/aliases HEL-S-98n
Species Homo sapiens
 Database cross references - COMT
ExoCarta ExoCarta_1312
Entrez Gene 1312
HGNC 2228
MIM 116790
UniProt P21964  
 COMT identified in exosomes derived from the following tissue/cell type
Breast cancer cells 19415654    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for COMT
Molecular Function
    magnesium ion binding GO:0000287 IEA
    O-methyltransferase activity GO:0008171 TAS
    catechol O-methyltransferase activity GO:0016206 IEA
    protein binding GO:0005515 IPI
Biological Process
    small molecule metabolic process GO:0044281 TAS
    positive regulation of homocysteine metabolic process GO:0050668 IEA
    dopamine catabolic process GO:0042420 IEA
    negative regulation of renal sodium excretion GO:0035814 IEA
    synaptic transmission GO:0007268 TAS
    negative regulation of dopamine metabolic process GO:0045963 IEA
    xenobiotic metabolic process GO:0006805 TAS
    negative regulation of smooth muscle cell proliferation GO:0048662 IEA
    response to pain GO:0048265 IEA
    short-term memory GO:0007614 IEA
    multicellular organismal reproductive process GO:0048609 IEA
    methylation GO:0032259 TAS
    female pregnancy GO:0007565 IEA
    neurotransmitter catabolic process GO:0042135 IEA
    developmental process GO:0032502 IEA
    learning GO:0007612 IEA
    neurotransmitter biosynthetic process GO:0042136 TAS
    estrogen metabolic process GO:0008210 IEA
    response to organic cyclic compound GO:0014070 IEA
    regulation of sensory perception of pain GO:0051930 IEA
    response to drug GO:0042493 IEA
    cellular response to phosphate starvation GO:0016036 IEA
    response to lipopolysaccharide GO:0032496 IEA
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    plasma membrane GO:0005886 TAS
    cell body GO:0044297 IEA
    axon GO:0030424 IEA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IDA
    mitochondrion GO:0005739 IEA
    dendritic spine GO:0043197 IEA
    postsynaptic membrane GO:0045211 IEA
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified COMT in exosomes
1
Experiment ID 65
ISEV standards
✘ EV Biophysical techniques
✔
HSP60|HSP27|GAPDH
EV Cytosolic markers
✔
FLOT2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19415654    
Organism Homo sapiens
Experiment description Proteomics of MUC1-containing lipid rafts from plasma membranes and exosomes of human breast carcinoma cells MCF-7.
Authors Staubach S, Razawi H, Hanisch FG.
Journal name PROTEOMICS
Publication year 2009
Sample Breast cancer cells
Sample name MCF-7
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for COMT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 XRN2 22803
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
2 TRIP13 9319
Two-hybrid Homo sapiens
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