Gene description for IDE
Gene name insulin-degrading enzyme
Gene symbol IDE
Other names/aliases INSULYSIN
Species Homo sapiens
 Database cross references - IDE
ExoCarta ExoCarta_3416
Entrez Gene 3416
HGNC 5381
MIM 146680
UniProt P14735  
 IDE identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for IDE
Molecular Function
    ubiquitin binding GO:0043130 IPI
    metalloendopeptidase activity GO:0004222 IDA
    peptide binding GO:0042277 IPI
    insulin binding GO:0043559 IPI
    ATP binding GO:0005524 IDA
    beta-amyloid binding GO:0001540 IEA
    protein homodimerization activity GO:0042803 IPI
    glycoprotein binding GO:0001948 IPI
    beta-endorphin binding GO:0031626 IEA
    receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    ATPase activity GO:0016887 IEA
    zinc ion binding GO:0008270 IDA
Biological Process
    ubiquitin homeostasis GO:0010992 IDA
    bradykinin catabolic process GO:0010815 IDA
    negative regulation of proteolysis GO:0045861 IEA
    proteolysis involved in cellular protein catabolic process GO:0051603 IDA
    insulin metabolic process GO:1901142 IDA
    insulin receptor signaling pathway GO:0008286 NAS
    determination of adult lifespan GO:0008340 IDA
    insulin catabolic process GO:1901143 IDA
    protein homooligomerization GO:0051260 IDA
    positive regulation of protein oligomerization GO:0032461 IDA
    protein heterooligomerization GO:0051291 IEA
    proteolysis GO:0006508 IDA
    viral process GO:0016032 IEA
    beta-amyloid metabolic process GO:0050435 IDA
    protein homotetramerization GO:0051289 IEA
    hormone catabolic process GO:0042447 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    nucleoplasm GO:0005654 IDA
    nucleus GO:0005634 IDA
    peroxisome GO:0005777 IDA
    cytosolic proteasome complex GO:0031597 IEA
    peroxisomal matrix GO:0005782 IEA
    cytoplasm GO:0005737 IDA
    extracellular space GO:0005615 IDA
    plasma membrane GO:0005886 IEA
    cell surface GO:0009986 IDA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified IDE in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for IDE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGF2 3481
Reconstituted Complex Homo sapiens
2 IGF1  
Reconstituted Complex Homo sapiens
3 ZHX2  
Co-purification Homo sapiens
View the network image/svg+xml
 Pathways in which IDE is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here