Gene description for PAPSS1
Gene name 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Gene symbol PAPSS1
Other names/aliases ATPSK1
PAPSS
SK1
Species Homo sapiens
 Database cross references - PAPSS1
ExoCarta ExoCarta_9061
Vesiclepedia VP_9061
Entrez Gene 9061
HGNC 8603
MIM 603262
UniProt O43252  
 PAPSS1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PAPSS1
Molecular Function
    adenylylsulfate kinase activity GO:0004020 IBA
    adenylylsulfate kinase activity GO:0004020 IDA
    adenylylsulfate kinase activity GO:0004020 TAS
    sulfate adenylyltransferase (ATP) activity GO:0004781 IDA
    sulfate adenylyltransferase (ATP) activity GO:0004781 ISS
    sulfate adenylyltransferase (ATP) activity GO:0004781 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    nucleotidyltransferase activity GO:0016779 ISS
    protein homodimerization activity GO:0042803 IPI
Biological Process
    sulfate assimilation GO:0000103 IBA
    sulfate assimilation GO:0000103 IDA
    sulfate assimilation GO:0000103 IEA
    skeletal system development GO:0001501 TAS
    3'-phosphoadenosine 5'-phosphosulfate biosynthetic process GO:0050428 IBA
    3'-phosphoadenosine 5'-phosphosulfate biosynthetic process GO:0050428 IDA
    3'-phosphoadenosine 5'-phosphosulfate biosynthetic process GO:0050428 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PAPSS1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PAPSS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
2 PAFAH1B1 5048
Co-fractionation Homo sapiens
3 ARHGAP17 55114
Co-fractionation Homo sapiens
4 DHX15 1665
Co-fractionation Homo sapiens
5 NAPRT 93100
Co-fractionation Homo sapiens
6 GLS 2744
Co-fractionation Homo sapiens
7 CTPS1 1503
Co-fractionation Homo sapiens
8 TFCP2 7024
Affinity Capture-MS Homo sapiens
9 FASN 2194
Negative Genetic Homo sapiens
10 Tyw3  
Affinity Capture-MS Mus musculus
11 NCK1 4690
Affinity Capture-MS Homo sapiens
12 FANCG 2189
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 AHCY 191
Co-fractionation Homo sapiens
14 GPX7 2882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 KPNA1 3836
Affinity Capture-MS Homo sapiens
16 FAM24B  
Affinity Capture-MS Homo sapiens
17 NSFL1C 55968
Co-fractionation Homo sapiens
18 HMGB3 3149
Co-fractionation Homo sapiens
19 CCDC116  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PTMA 5757
Co-fractionation Homo sapiens
21 ASNS 440
Co-fractionation Homo sapiens
22 PSME2 5721
Co-fractionation Homo sapiens
23 DYNC1I2 1781
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MTRR  
Co-fractionation Homo sapiens
25 FERMT2 10979
Co-fractionation Homo sapiens
26 Papss1  
Affinity Capture-MS Mus musculus
27 ACAT2 39
Co-fractionation Homo sapiens
28 NAE1 8883
Co-fractionation Homo sapiens
29 IDE 3416
Co-fractionation Homo sapiens
30 PPP5C 5536
Co-fractionation Homo sapiens
31 TUBB3 10381
Co-fractionation Homo sapiens
32 C4orf26  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 FANCI 55215
Affinity Capture-MS Homo sapiens
34 RNASEH2A 10535
Affinity Capture-MS Homo sapiens
35 FBLIM1 54751
Affinity Capture-MS Homo sapiens
36 SREBF2 6721
Negative Genetic Homo sapiens
37 GNL1 2794
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 FAM195A  
Affinity Capture-MS Homo sapiens
39 PPP1R16A 84988
Two-hybrid Homo sapiens
40 PRPS2 5634
Affinity Capture-MS Homo sapiens
41 TRIM29 23650
Co-fractionation Homo sapiens
42 FGB 2244
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 MAPKAPK3 7867
Co-fractionation Homo sapiens
44 CAPZB 832
Co-fractionation Homo sapiens
45 GALNT3 2591
Affinity Capture-MS Homo sapiens
46 PARK2  
Affinity Capture-MS Homo sapiens
47 FBXW7  
Affinity Capture-MS Homo sapiens
48 ALKBH5 54890
Co-fractionation Homo sapiens
49 TMEM132A 54972
Affinity Capture-MS Homo sapiens
50 RPA3 6119
Proximity Label-MS Homo sapiens
51 ERRFI1 54206
Two-hybrid Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 Rhoa 11848
Affinity Capture-MS Mus musculus
54 RPL35A 6165
Affinity Capture-MS Homo sapiens
55 TBC1D13 54662
Co-fractionation Homo sapiens
56 XPOT 11260
Co-fractionation Homo sapiens
57 FH 2271
Co-fractionation Homo sapiens
58 UNC119 9094
Two-hybrid Homo sapiens
59 PARP1 142
Affinity Capture-MS Homo sapiens
60 SNRNP27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 UBA3 9039
Co-fractionation Homo sapiens
62 MSN 4478
Co-fractionation Homo sapiens
63 ASMTL 8623
Affinity Capture-MS Homo sapiens
64 YAF2  
Affinity Capture-MS Homo sapiens
65 CNDP2 55748
Co-fractionation Homo sapiens
66 SMOC1 64093
Affinity Capture-MS Homo sapiens
67 C2orf44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PAPSS2 9060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
69 ATIC 471
Co-fractionation Homo sapiens
70 TATDN1 83940
Co-fractionation Homo sapiens
71 CAPN2 824
Co-fractionation Homo sapiens
72 EEF1A1 1915
Two-hybrid Homo sapiens
View the network image/svg+xml



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