Gene description for PAPSS1
Gene name 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Gene symbol PAPSS1
Other names/aliases ATPSK1
PAPSS
SK1
Species Homo sapiens
 Database cross references - PAPSS1
ExoCarta ExoCarta_9061
Entrez Gene 9061
HGNC 8603
MIM 603262
UniProt O43252  
 PAPSS1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for PAPSS1
Molecular Function
    sulfate adenylyltransferase (ATP) activity GO:0004781 ISS
    nucleotidyltransferase activity GO:0016779 ISS
    ATP binding GO:0005524 IEA
    adenylylsulfate kinase activity GO:0004020 IBA
Biological Process
    3'-phosphoadenosine 5'-phosphosulfate biosynthetic process GO:0050428 TAS
    skeletal system development GO:0001501 TAS
    phosphorylation GO:0016310 IEA
    carbohydrate metabolic process GO:0005975 TAS
    sulfate assimilation GO:0000103 IEA
    xenobiotic metabolic process GO:0006805 TAS
    3'-phosphoadenosine 5'-phosphosulfate metabolic process GO:0050427 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PAPSS1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PAPSS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP1R16A  
Two-hybrid Homo sapiens
2 EEF1A1 1915
Two-hybrid Homo sapiens
3 UNC119  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PAPSS1 is involved
PathwayEvidenceSource
Transport and synthesis of PAPS TAS Reactome
Transport and synthesis of PAPS TAS Reactome





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