Gene description for RNASEH2A
Gene name ribonuclease H2, subunit A
Gene symbol RNASEH2A
Other names/aliases AGS4
JUNB
RNASEHI
RNHIA
RNHL
Species Homo sapiens
 Database cross references - RNASEH2A
ExoCarta ExoCarta_10535
Entrez Gene 10535
HGNC 18518
MIM 606034
UniProt O75792  
 RNASEH2A identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for RNASEH2A
Molecular Function
    RNA-DNA hybrid ribonuclease activity GO:0004523 IDA
    ribonuclease activity GO:0004540 TAS
    metal ion binding GO:0046872 IEA
    RNA binding GO:0003723 IEA
Biological Process
    RNA phosphodiester bond hydrolysis GO:0090501 TAS
    RNA catabolic process GO:0006401 IDA
    mismatch repair GO:0006298 IDA
    DNA replication GO:0006260 TAS
    DNA replication, removal of RNA primer GO:0043137 IBA
    RNA phosphodiester bond hydrolysis, endonucleolytic GO:0090502 IEA
Subcellular Localization
    ribonuclease H2 complex GO:0032299 IDA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified RNASEH2A in exosomes
1
Experiment ID 236
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RNASEH2A
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which RNASEH2A is involved
No pathways found





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