Gene description for SETD3
Gene name SET domain containing 3
Gene symbol SETD3
Other names/aliases C14orf154
Species Homo sapiens
 Database cross references - SETD3
ExoCarta ExoCarta_84193
Vesiclepedia VP_84193
Entrez Gene 84193
HGNC 20493
MIM 615671
UniProt Q86TU7  
 SETD3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SETD3
Molecular Function
    transcription coactivator activity GO:0003713 IBA
    transcription coactivator activity GO:0003713 ISS
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    protein-lysine N-methyltransferase activity GO:0016279 IDA
    protein-L-histidine N-tele-methyltransferase activity GO:0018064 IBA
    protein-L-histidine N-tele-methyltransferase activity GO:0018064 IDA
    histone H3K4 methyltransferase activity GO:0042800 IBA
    histone H3K36 methyltransferase activity GO:0046975 IBA
    histone H3K36 methyltransferase activity GO:0046975 ISS
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    peptidyl-histidine methylation GO:0018021 IDA
    peptidyl-lysine trimethylation GO:0018023 IDA
    actin modification GO:0030047 IBA
    actin modification GO:0030047 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of muscle cell differentiation GO:0051149 IEA
    regulation of uterine smooth muscle contraction GO:0070472 IBA
    regulation of uterine smooth muscle contraction GO:0070472 ISS
Subcellular Localization
    chromatin GO:0000785 IEA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified SETD3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SETD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LRRC15 131578
Affinity Capture-MS Homo sapiens
2 BPHL 670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TEX13A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SNX3 8724
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GMFB 2764
Co-fractionation Homo sapiens
6 APLNR  
Two-hybrid Homo sapiens
7 ITSN1 6453
Co-fractionation Homo sapiens
8 MSH2 4436
Co-fractionation Homo sapiens
9 XPO1 7514
Co-fractionation Homo sapiens
10 KCTD10 83892
Affinity Capture-MS Homo sapiens
11 RFC2 5982
Affinity Capture-MS Homo sapiens
12 CMTR1 23070
Co-fractionation Homo sapiens
13 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
14 PARK2  
Affinity Capture-MS Homo sapiens
15 APP 351
Reconstituted Complex Homo sapiens
16 MYOD1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 TCEA1 6917
Co-fractionation Homo sapiens
18 RNASEH2A 10535
Co-fractionation Homo sapiens
19 RPA3 6119
Proximity Label-MS Homo sapiens
20 NDOR1  
Co-fractionation Homo sapiens
21 PMVK 10654
Affinity Capture-MS Homo sapiens
22 GFPT1 2673
Co-fractionation Homo sapiens
23 RPA4  
Proximity Label-MS Homo sapiens
24 RFC4 5984
Affinity Capture-MS Homo sapiens
25 RAD21 5885
Affinity Capture-Western Homo sapiens
26 SRPK1 6732
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which SETD3 is involved
PathwayEvidenceSource
Chromatin modifying enzymes TAS Reactome
Chromatin organization TAS Reactome
PKMTs methylate histone lysines TAS Reactome





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