Gene description for RFC4
Gene name replication factor C (activator 1) 4, 37kDa
Gene symbol RFC4
Other names/aliases A1
RFC37
Species Homo sapiens
 Database cross references - RFC4
ExoCarta ExoCarta_5984
Entrez Gene 5984
HGNC 9972
MIM 102577
UniProt P35249  
 RFC4 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for RFC4
Molecular Function
    ATP binding GO:0005524 IEA
    DNA clamp loader activity GO:0003689 IBA
    protein binding GO:0005515 IPI
Biological Process
    telomere maintenance GO:0000723 TAS
    translesion synthesis GO:0019985 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    error-prone translesion synthesis GO:0042276 TAS
    nucleotide-excision repair GO:0006289 TAS
    DNA-dependent DNA replication GO:0006261 IBA
    DNA replication GO:0006260 TAS
    DNA damage response, detection of DNA damage GO:0042769 TAS
    mitotic cell cycle GO:0000278 TAS
    error-free translesion synthesis GO:0070987 TAS
    telomere maintenance via recombination GO:0000722 TAS
    nucleotide-excision repair, DNA gap filling GO:0006297 TAS
    telomere maintenance via semi-conservative replication GO:0032201 TAS
    DNA strand elongation involved in DNA replication GO:0006271 TAS
    base-excision repair GO:0006284 TAS
    DNA repair GO:0006281 TAS
Subcellular Localization
    DNA replication factor C complex GO:0005663 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified RFC4 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RFC4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RFC3 5983
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
2 PCNA 5111
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
3 CHTF18 63922
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
4 RFC2 5982
Co-purification Homo sapiens
Co-purification Homo sapiens
5 RFC5 5985
Co-purification Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
6 CDK4 1019
Affinity Capture-MS Homo sapiens
7 HDAC1 3065
Affinity Capture-MS Homo sapiens
8 RAD17  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
9 BRD4 23476
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
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