Gene description for NOTCH1
Gene name notch 1
Gene symbol NOTCH1
Other names/aliases AOS5
AOVD1
TAN1
hN1
Species Homo sapiens
 Database cross references - NOTCH1
ExoCarta ExoCarta_4851
Vesiclepedia VP_4851
Entrez Gene 4851
HGNC 7881
MIM 190198
UniProt P46531  
 NOTCH1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NOTCH1
Molecular Function
    protein binding GO:0005515 IPI
    calcium ion binding GO:0005509 IEA
    chromatin DNA binding GO:0031490 IEA
    enzyme inhibitor activity GO:0004857 ISS
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    core promoter binding GO:0001047 ISS
    sequence-specific DNA binding GO:0043565 IEA
    RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription GO:0001190 ISS
    enzyme binding GO:0019899 ISS
    receptor activity GO:0004872 IEA
Biological Process
    endocardial cushion morphogenesis GO:0003203 ISS
    positive regulation of transcription, DNA-templated GO:0045893 ISS
    forebrain development GO:0030900 IEA
    negative regulation of cell migration involved in sprouting angiogenesis GO:0090051 IDA
    glomerular mesangial cell development GO:0072144 IEA
    spermatogenesis GO:0007283 IEA
    negative regulation of neurogenesis GO:0050768 ISS
    prostate gland epithelium morphogenesis GO:0060740 IEA
    cardiac epithelial to mesenchymal transition GO:0060317 ISS
    positive regulation of cell migration GO:0030335 ISS
    positive regulation of cell proliferation GO:0008284 IMP
    endoderm development GO:0007492 IEA
    negative regulation of ossification GO:0030279 ISS
    astrocyte differentiation GO:0048708 IEA
    regulation of somitogenesis GO:0014807 IEA
    hair follicle morphogenesis GO:0031069 IEA
    inflammatory response to antigenic stimulus GO:0002437 IEA
    distal tubule development GO:0072017 IEA
    regulation of epithelial cell proliferation involved in prostate gland development GO:0060768 IEA
    branching morphogenesis of an epithelial tube GO:0048754 IEA
    positive regulation of cardiac muscle cell proliferation GO:0060045 ISS
    coronary artery morphogenesis GO:0060982 ISS
    lung development GO:0030324 IEA
    Notch receptor processing GO:0007220 TAS
    cardiac right ventricle formation GO:0003219 IEA
    negative regulation of BMP signaling pathway GO:0030514 ISS
    regulation of extracellular matrix assembly GO:1901201 ISS
    axonogenesis GO:0007409 IEA
    negative regulation of calcium ion-dependent exocytosis GO:0045955 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    tissue regeneration GO:0042246 IEA
    Notch signaling involved in heart development GO:0061314 IMP
    endocardium morphogenesis GO:0003160 ISS
    left/right axis specification GO:0070986 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    response to lipopolysaccharide GO:0032496 IEA
    mitral valve formation GO:0003192 IMP
    negative regulation of oligodendrocyte differentiation GO:0048715 ISS
    endocardial cell differentiation GO:0060956 ISS
    oligodendrocyte differentiation GO:0048709 IEA
    tube formation GO:0035148 IMP
    compartment pattern specification GO:0007386 IEA
    positive regulation of JAK-STAT cascade GO:0046427 ISS
    arterial endothelial cell differentiation GO:0060842 ISS
    interleukin-4 secretion GO:0072602 IEA
    cardiac septum morphogenesis GO:0060411 ISS
    cardiac ventricle morphogenesis GO:0003208 ISS
    atrioventricular valve morphogenesis GO:0003181 ISS
    cardiac vascular smooth muscle cell development GO:0060948 ISS
    response to corticosteroid GO:0031960 IEA
    cardiac chamber formation GO:0003207 ISS
    negative regulation of osteoblast differentiation GO:0045668 ISS
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IDA
    growth involved in heart morphogenesis GO:0003241 ISS
    apoptotic process involved in embryonic digit morphogenesis GO:1902263 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISS
    cardiac muscle cell proliferation GO:0060038 IEA
    auditory receptor cell fate commitment GO:0009912 IEA
    regulation of transcription, DNA-templated GO:0006355 TAS
    atrioventricular node development GO:0003162 IEA
    cell fate specification GO:0001708 IEA
    foregut morphogenesis GO:0007440 IEA
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    collecting duct development GO:0072044 IEA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IMP
    keratinocyte differentiation GO:0030216 IEA
    coronary vein morphogenesis GO:0003169 ISS
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    negative regulation of cell proliferation GO:0008285 IDA
    ventricular septum morphogenesis GO:0060412 IMP
    gene expression GO:0010467 TAS
    negative regulation of glial cell proliferation GO:0060253 ISS
    positive regulation of astrocyte differentiation GO:0048711 ISS
    positive regulation of apoptotic process GO:0043065 IEA
    pericardium morphogenesis GO:0003344 ISS
    positive regulation of keratinocyte differentiation GO:0045618 IEA
    cardiac right atrium morphogenesis GO:0003213 ISS
    determination of left/right symmetry GO:0007368 ISS
    aortic valve morphogenesis GO:0003180 IMP
    negative regulation of catalytic activity GO:0043086 ISS
    neural tube development GO:0021915 IEA
    cardiac muscle tissue morphogenesis GO:0055008 ISS
    negative regulation of endothelial cell chemotaxis GO:2001027 IDA
    cilium morphogenesis GO:0060271 ISS
    immune response GO:0006955 NAS
    negative regulation of stem cell differentiation GO:2000737 IMP
    epithelial to mesenchymal transition GO:0001837 ISS
    Notch signaling pathway involved in regulation of secondary heart field cardioblast proliferation GO:0003270 IEA
    positive regulation of transcription of Notch receptor target GO:0007221 ISS
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    negative regulation of anoikis GO:2000811 IMP
    organ regeneration GO:0031100 IEA
    heart development GO:0007507 IMP
    heart trabecula morphogenesis GO:0061384 ISS
    epithelial to mesenchymal transition involved in endocardial cushion formation GO:0003198 ISS
    negative regulation of myotube differentiation GO:0010832 ISS
    vasculogenesis involved in coronary vascular morphogenesis GO:0060979 ISS
    venous endothelial cell differentiation GO:0060843 ISS
    liver development GO:0001889 IEA
    regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation GO:0003256 ISS
    negative regulation of cell-substrate adhesion GO:0010812 IDA
    humoral immune response GO:0006959 IEA
    ventricular trabecula myocardium morphogenesis GO:0003222 ISS
    mesenchymal cell development GO:0014031 ISS
    heart looping GO:0001947 ISS
    negative regulation of photoreceptor cell differentiation GO:0046533 IEA
    negative regulation of pro-B cell differentiation GO:2000974 ISS
    pulmonary valve morphogenesis GO:0003184 IMP
    cardiac left ventricle morphogenesis GO:0003214 ISS
    embryonic hindlimb morphogenesis GO:0035116 IEA
    positive regulation of BMP signaling pathway GO:0030513 ISS
    positive regulation of endothelial cell differentiation GO:0045603 IEA
    in utero embryonic development GO:0001701 IEA
    somatic stem cell division GO:0048103 IEA
    cellular response to follicle-stimulating hormone stimulus GO:0071372 IDA
    sprouting angiogenesis GO:0002040 IEA
    skeletal muscle cell differentiation GO:0035914 IEA
    endocardium development GO:0003157 ISS
    cardiac atrium morphogenesis GO:0003209 ISS
    neuronal stem cell maintenance GO:0097150 IEP
    Notch signaling pathway GO:0007219 TAS
    positive regulation of neuroblast proliferation GO:0002052 IEA
    secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development GO:0060528 IEA
    negative regulation of myoblast differentiation GO:0045662 IMP
    cell migration involved in endocardial cushion formation GO:0003273 ISS
    positive regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061419 ISS
    response to muramyl dipeptide GO:0032495 IEA
    cell differentiation in spinal cord GO:0021515 IEA
Subcellular Localization
    plasma membrane GO:0005886 TAS
    cytosol GO:0005829 TAS
    receptor complex GO:0043235 IDA
    extracellular region GO:0005576 TAS
    integral component of membrane GO:0016021 IEA
    nucleus GO:0005634 TAS
    cell surface GO:0009986 IEA
    acrosomal vesicle GO:0001669 IEA
    MAML1-RBP-Jkappa- ICN1 complex GO:0002193 IDA
    Golgi membrane GO:0000139 TAS
    nucleoplasm GO:0005654 TAS
    endoplasmic reticulum membrane GO:0005789 TAS
 Experiment description of studies that identified NOTCH1 in exosomes
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NOTCH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RELA 5970
Affinity Capture-Western Homo sapiens
2 RBPJ 3516
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 NOTCH3 4854
Phenotypic Suppression Homo sapiens
4 PSEN1 5663
Invivo Homo sapiens
5 LFNG 3955
Invivo Homo sapiens
6 GSK3B 2932
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
7 NUMB 8650
Reconstituted Complex Homo sapiens
8 MAML1  
Invivo Homo sapiens
Invitro Homo sapiens
9 LCK 3932
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 YY1 7528
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 NOV 4856
Invivo Homo sapiens
12 DTX1  
Affinity Capture-Western Homo sapiens
13 DLL1  
Reconstituted Complex Homo sapiens
14 MFNG  
Invivo Homo sapiens
15 JAG2  
Reconstituted Complex Homo sapiens
16 DLL4  
Invivo Homo sapiens
17 MAML2  
Invivo Homo sapiens
18 SMAD3 4088
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 CNTN1 1272
Affinity Capture-Western Homo sapiens
20 PIK3CA 5290
Affinity Capture-Western Homo sapiens
21 NFKB1 4790
Affinity Capture-Western Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
22 FBXW7  
Invivo Homo sapiens
Invitro Homo sapiens
23 XRCC6 2547
Two-hybrid Homo sapiens
24 SNW1 22938
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
25 MAML3  
Invivo Homo sapiens
26 LEF1  
Reconstituted Complex Homo sapiens
27 JAG1 182
Reconstituted Complex Homo sapiens
28 NOTCH1 4851
Invivo Homo sapiens
Invivo Homo sapiens
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