Gene description for MDN1
Gene name MDN1, midasin homolog (yeast)
Gene symbol MDN1
Other names/aliases -
Species Homo sapiens
 Database cross references - MDN1
ExoCarta ExoCarta_23195
Entrez Gene 23195
HGNC 18302
UniProt Q9NU22  
 MDN1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for MDN1
Molecular Function
    ATPase activity GO:0016887 NAS
    unfolded protein binding GO:0051082 NAS
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
Biological Process
    metabolic process GO:0008152 NAS
    protein complex assembly GO:0006461 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 NAS
    nucleolus GO:0005730 IDA
    intermediate filament cytoskeleton GO:0045111 IDA
    membrane GO:0016020 IDA
 Experiment description of studies that identified MDN1 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MDN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which MDN1 is involved
No pathways found





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