Gene description for MDN1
Gene name MDN1, midasin homolog (yeast)
Gene symbol MDN1
Other names/aliases -
Species Homo sapiens
 Database cross references - MDN1
ExoCarta ExoCarta_23195
Vesiclepedia VP_23195
Entrez Gene 23195
HGNC 18302
UniProt Q9NU22  
 MDN1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for MDN1
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
Biological Process
    ribosomal large subunit assembly GO:0000027 IBA
    ribosomal large subunit assembly GO:0000027 IDA
    ribosomal large subunit export from nucleus GO:0000055 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    membrane GO:0016020 HDA
    preribosome, large subunit precursor GO:0030687 IBA
    intermediate filament cytoskeleton GO:0045111 IDA
 Experiment description of studies that identified MDN1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MDN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UBE2H 7328
Affinity Capture-MS Homo sapiens
4 SENP3 26168
Affinity Capture-MS Homo sapiens
5 SET 6418
Co-fractionation Homo sapiens
6 LAMP3  
Proximity Label-MS Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 Chmp4b 75608
Affinity Capture-MS Mus musculus
9 UBC 7316
Reconstituted Complex Homo sapiens
10 DPEP1 1800
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GSK3A 2931
Affinity Capture-MS Homo sapiens
12 KIAA1429 25962
Affinity Capture-MS Homo sapiens
13 WDR76  
Affinity Capture-MS Homo sapiens
14 CHODL 140578
Affinity Capture-MS Homo sapiens
15 HLA-B 3106
Affinity Capture-MS Homo sapiens
16 WDR83  
Affinity Capture-MS Homo sapiens
17 GLMP 112770
Affinity Capture-MS Homo sapiens
18 KLRC1  
Affinity Capture-MS Homo sapiens
19 POLR1E  
Proximity Label-MS Homo sapiens
20 OBSL1 23363
Affinity Capture-MS Homo sapiens
21 ABCE1 6059
Affinity Capture-MS Homo sapiens
22 EXD2  
Proximity Label-MS Homo sapiens
23 TACSTD2 4070
Affinity Capture-MS Homo sapiens
24 BRD4 23476
Affinity Capture-MS Homo sapiens
25 DNAJC7 7266
Proximity Label-MS Homo sapiens
26 GPR17 2840
Affinity Capture-MS Homo sapiens
27 Tmed10 68581
Affinity Capture-MS Mus musculus
28 FAS 355
Affinity Capture-MS Homo sapiens
29 SSR2  
Affinity Capture-MS Homo sapiens
30 BRIX1 55299
Co-fractionation Homo sapiens
31 Wiz  
Affinity Capture-MS Mus musculus
32 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 AURKA 6790
Affinity Capture-MS Homo sapiens
34 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
37 CTDP1 9150
Affinity Capture-MS Homo sapiens
38 RB1CC1 9821
Affinity Capture-MS Homo sapiens
39 ILVBL 10994
Affinity Capture-MS Homo sapiens
40 VCP 7415
Affinity Capture-MS Homo sapiens
41 NPTN 27020
Affinity Capture-MS Homo sapiens
42 GPR182  
Affinity Capture-MS Homo sapiens
43 SUMO2 6613
Reconstituted Complex Homo sapiens
44 RPA4  
Proximity Label-MS Homo sapiens
45 PML 5371
Affinity Capture-MS Homo sapiens
46 HECTD1 25831
Affinity Capture-MS Homo sapiens
47 DGCR2 9993
Affinity Capture-MS Homo sapiens
48 PELP1 27043
Co-fractionation Homo sapiens
49 UBXN6 80700
Affinity Capture-MS Homo sapiens
50 CD3EAP  
Proximity Label-MS Homo sapiens
51 TUBB 203068
Affinity Capture-MS Homo sapiens
52 APLNR  
Affinity Capture-MS Homo sapiens
53 SMARCAD1  
Affinity Capture-MS Homo sapiens
54 SIRT7  
Affinity Capture-MS Homo sapiens
55 EPB41L4A 64097
Proximity Label-MS Homo sapiens
56 STK17B 9262
Affinity Capture-MS Homo sapiens
57 CUL3 8452
Affinity Capture-MS Homo sapiens
58 NR3C1 2908
Proximity Label-MS Homo sapiens
59 FOXL1  
Affinity Capture-MS Homo sapiens
60 METTL21B  
Affinity Capture-MS Homo sapiens
61 ATG14  
Affinity Capture-MS Homo sapiens
62 RIPK4  
Affinity Capture-MS Homo sapiens
63 FN1 2335
Affinity Capture-MS Homo sapiens
64 C3orf18  
Affinity Capture-MS Homo sapiens
65 RPA3 6119
Proximity Label-MS Homo sapiens
66 ATP2A1 487
Proximity Label-MS Homo sapiens
67 GPR45  
Affinity Capture-MS Homo sapiens
68 SART3 9733
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
69 SCN2B  
Affinity Capture-MS Homo sapiens
70 CUL7 9820
Affinity Capture-MS Homo sapiens
71 DDRGK1 65992
Affinity Capture-MS Homo sapiens
72 METTL9 51108
Affinity Capture-MS Homo sapiens
73 ISLR 3671
Affinity Capture-MS Homo sapiens
74 PEX3 8504
Proximity Label-MS Homo sapiens
75 AKAP1 8165
Proximity Label-MS Homo sapiens
76 PARP16 54956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CCDC8  
Affinity Capture-MS Homo sapiens
78 Anapc2  
Affinity Capture-MS Mus musculus
79 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
80 ART3  
Affinity Capture-MS Homo sapiens
81 TMEM9 252839
Affinity Capture-MS Homo sapiens
82 PARP1 142
Proximity Label-MS Homo sapiens
83 OPALIN  
Affinity Capture-MS Homo sapiens
84 SRP19 6728
Co-fractionation Homo sapiens
85 TEX10 54881
Co-fractionation Homo sapiens
86 TUBB3 10381
Co-fractionation Homo sapiens
87 SHFM1 7979
Affinity Capture-MS Homo sapiens
88 NIFK 84365
Proximity Label-MS Homo sapiens
89 LATS1  
Proximity Label-MS Homo sapiens
90 ERGIC2 51290
Proximity Label-MS Homo sapiens
91 BECN1 8678
Affinity Capture-MS Homo sapiens
92 LRRK2 120892
Affinity Capture-MS Homo sapiens
93 SNW1 22938
Affinity Capture-MS Homo sapiens
94 KRAS 3845
Negative Genetic Homo sapiens
95 C9orf72  
Affinity Capture-MS Homo sapiens
96 AR 367
Two-hybrid Homo sapiens
97 ATOH1  
Affinity Capture-MS Homo sapiens
98 CCT8L2  
Affinity Capture-MS Homo sapiens
99 NOTCH1 4851
Affinity Capture-MS Homo sapiens
100 CDC5L 988
Affinity Capture-MS Homo sapiens
101 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 Chmp3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which MDN1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here