Gene description for SET
Gene name SET nuclear proto-oncogene
Gene symbol SET
Other names/aliases 2PP2A
I2PP2A
IGAAD
IPP2A2
PHAPII
TAF-I
TAF-IBETA
Species Homo sapiens
 Database cross references - SET
ExoCarta ExoCarta_6418
Entrez Gene 6418
HGNC 10760
MIM 600960
UniProt Q01105  
 SET identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 25844599    
 Gene ontology annotations for SET
Molecular Function
    protein phosphatase inhibitor activity GO:0004864 TAS
    protein phosphatase type 2A regulator activity GO:0008601 TAS
    histone binding GO:0042393 TAS
    protein binding GO:0005515 IPI
    DNA binding GO:0003677 IEA
Biological Process
    regulation of protein phosphatase type 2A activity GO:0034047 TAS
    mitotic cell cycle GO:0000278 TAS
    negative regulation of neuron apoptotic process GO:0043524 IGI
    negative regulation of histone acetylation GO:0035067 TAS
    negative regulation of catalytic activity GO:0043086 TAS
    viral process GO:0016032 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IDA
    nucleosome disassembly GO:0006337 TAS
    nucleosome assembly GO:0006334 IEA
    nucleocytoplasmic transport GO:0006913 NAS
    DNA replication GO:0006260 TAS
    gene expression GO:0010467 TAS
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    nucleoplasm GO:0005654 TAS
    protein complex GO:0043234 IDA
    cytosol GO:0005829 IEA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    endoplasmic reticulum GO:0005783 IDA
 Experiment description of studies that identified SET in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 140
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
7
Experiment ID 141
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
8
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for SET
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST3H3 8290
Invivo Homo sapiens
Invitro Homo sapiens
2 DGKE  
Affinity Capture-MS Homo sapiens
3 THAP7  
Two-hybrid Homo sapiens
4 RGS20 8601
Affinity Capture-MS Homo sapiens
5 APEX1 328
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 CDK5R1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 GZMA 3001
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
8 NME1 4830
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 ANP32A 8125
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
10 CDKN1A  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
11 CDK5 1020
Affinity Capture-MS Homo sapiens
12 KLF5  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 PTMA 5757
Affinity Capture-MS Homo sapiens
14 HMGB2 3148
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
15 SETBP1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
16 TAF1A  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SET is involved
PathwayEvidenceSource
Condensation of Prophase Chromosomes TAS Reactome
HuR stabilizes mRNA TAS Reactome





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