Gene description for HSPA14
Gene name heat shock 70kDa protein 14
Gene symbol HSPA14
Other names/aliases HSP70-4
HSP70L1
Species Homo sapiens
 Database cross references - HSPA14
ExoCarta ExoCarta_51182
Vesiclepedia VP_51182
Entrez Gene 51182
HGNC 29526
MIM 610369
UniProt Q0VDF9  
 HSPA14 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HSPA14
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IBA
    heat shock protein binding GO:0031072 IBA
    protein folding chaperone GO:0044183 IBA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein refolding GO:0042026 IBA
    'de novo' cotranslational protein folding GO:0051083 TAS
    chaperone cofactor-dependent protein refolding GO:0051085 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    ribosome GO:0005840 IBA
    ribosome GO:0005840 IDA
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 HDA
 Experiment description of studies that identified HSPA14 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HSPA14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCT8L2  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 NFAM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 STK10 6793
Co-fractionation Homo sapiens
5 WIF1 11197
Affinity Capture-MS Homo sapiens
6 KIAA0101  
Co-fractionation Homo sapiens
7 FASN 2194
Positive Genetic Homo sapiens
8 PRKY  
Affinity Capture-MS Homo sapiens
9 PAXIP1  
Two-hybrid Homo sapiens
10 MSH2 4436
Co-fractionation Homo sapiens
11 FAS 355
Proximity Label-MS Homo sapiens
12 DNAJC2 27000
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 E2F4  
Affinity Capture-MS Homo sapiens
14 TRIM35 23087
Affinity Capture-MS Homo sapiens
15 ASIC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 POLR3H  
Affinity Capture-MS Homo sapiens
17 CC2D1B 200014
Affinity Capture-MS Homo sapiens
18 MZT2B 80097
Affinity Capture-MS Homo sapiens
19 BRCA1 672
Two-hybrid Homo sapiens
20 HYPK 25764
Affinity Capture-MS Homo sapiens
21 WRAP53  
Affinity Capture-MS Homo sapiens
22 ATP5F1 515
Affinity Capture-MS Homo sapiens
23 STIM1 6786
Affinity Capture-MS Homo sapiens
24 PDIA3 2923
Proximity Label-MS Homo sapiens
25 DNAJC17  
Co-fractionation Homo sapiens
26 FKBP8 23770
Proximity Label-MS Homo sapiens
27 NUDT14 256281
Affinity Capture-MS Homo sapiens
28 PARK2  
Affinity Capture-MS Homo sapiens
29 FOXL1  
Affinity Capture-MS Homo sapiens
30 METTL21B  
Affinity Capture-MS Homo sapiens
31 DRG1 4733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MILR1  
Affinity Capture-MS Homo sapiens
33 RPS6KB2  
Affinity Capture-MS Homo sapiens
34 S100A13 6284
Affinity Capture-MS Homo sapiens
35 ADAM19 8728
Affinity Capture-MS Homo sapiens
36 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
37 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
38 DNAJB6 10049
Affinity Capture-MS Homo sapiens
39 ATF6B  
Affinity Capture-MS Homo sapiens
40 RELL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 C22orf15  
Affinity Capture-MS Homo sapiens
42 DNAJC5 80331
Affinity Capture-MS Homo sapiens
43 KDM4B  
Affinity Capture-MS Homo sapiens
44 CYB5A 1528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 RPA3 6119
Proximity Label-MS Homo sapiens
46 BARD1 580
Two-hybrid Homo sapiens
47 OGT 8473
Reconstituted Complex Homo sapiens
48 SET 6418
Affinity Capture-MS Homo sapiens
49 SURF6  
Affinity Capture-MS Homo sapiens
50 DEFA5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TAB2  
Affinity Capture-MS Homo sapiens
52 XAGE1B  
Affinity Capture-MS Homo sapiens
53 C9orf170  
Affinity Capture-MS Homo sapiens
54 LDLR 3949
Positive Genetic Homo sapiens
55 DNAJC3 5611
Co-fractionation Homo sapiens
56 GDI1 2664
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 LGI1  
Affinity Capture-MS Homo sapiens
58 SNRNP27  
Affinity Capture-MS Homo sapiens
59 HECTD1 25831
Affinity Capture-MS Homo sapiens
60 PAF1 54623
Co-fractionation Homo sapiens
61 GPX7 2882
Affinity Capture-MS Homo sapiens
62 MAP3K7  
Affinity Capture-MS Homo sapiens
63 Sept9  
Affinity Capture-MS Mus musculus
64 RWDD1 51389
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
65 STIP1 10963
Reconstituted Complex Homo sapiens
66 GMFG 9535
Affinity Capture-MS Homo sapiens
67 DRG2 1819
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
68 UBXN6 80700
Affinity Capture-MS Homo sapiens
69 USP32 84669
Affinity Capture-MS Homo sapiens
70 KIF2C 11004
Co-fractionation Homo sapiens
View the network image/svg+xml



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