Gene description for DNAJB6
Gene name DnaJ (Hsp40) homolog, subfamily B, member 6
Gene symbol DNAJB6
Other names/aliases DJ4
DnaJ
HHDJ1
HSJ-2
HSJ2
LGMD1D
LGMD1E
MRJ
MSJ-1
Species Homo sapiens
 Database cross references - DNAJB6
ExoCarta ExoCarta_10049
Entrez Gene 10049
HGNC 14888
MIM 611332
UniProt O75190  
 DNAJB6 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for DNAJB6
Molecular Function
    ATPase activator activity GO:0001671 IDA
    protein binding GO:0005515 IPI
    unfolded protein binding GO:0051082 IDA
    chaperone binding GO:0051087 IPI
    heat shock protein binding GO:0031072 IDA
    DNA binding GO:0003677 IEA
Biological Process
    intermediate filament organization GO:0045109 IDA
    protein folding GO:0006457 IDA
    extracellular matrix organization GO:0030198 IEA
    positive regulation of ATPase activity GO:0032781 IDA
    chorion development GO:0060717 IEA
    syncytiotrophoblast cell differentiation involved in labyrinthine layer development GO:0060715 IEA
    actin cytoskeleton organization GO:0030036 IEA
    regulation of cellular response to heat GO:1900034 TAS
    cellular response to heat GO:0034605 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IEA
    negative regulation of inclusion body assembly GO:0090084 IDA
    protein localization to nucleus GO:0034504 IEA
    chorio-allantoic fusion GO:0060710 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 IDA
    cytosol GO:0005829 TAS
    Z disc GO:0030018 IDA
 Experiment description of studies that identified DNAJB6 in exosomes
1
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNAJB6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT18 3875
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJB6 is involved
PathwayEvidenceSource
Regulation of HSF1-mediated heat shock response TAS Reactome





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