Gene description for RNF20
Gene name ring finger protein 20, E3 ubiquitin protein ligase
Gene symbol RNF20
Other names/aliases BRE1
BRE1A
hBRE1
Species Homo sapiens
 Database cross references - RNF20
ExoCarta ExoCarta_56254
Vesiclepedia VP_56254
Entrez Gene 56254
HGNC 10062
MIM 607699
UniProt Q5VTR2  
 RNF20 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Thymus 23844026    
 Gene ontology annotations for RNF20
Molecular Function
    p53 binding GO:0002039 IDA
    chromatin binding GO:0003682 IEA
    transcription coactivator activity GO:0003713 IDA
    mRNA 3'-UTR binding GO:0003730 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    histone binding GO:0042393 IDA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    histone H2B C-terminal K residue ubiquitin ligase activity GO:0140850 IMP
Biological Process
    protein polyubiquitination GO:0000209 IDA
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated transcription GO:0006355 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    regulation of mitotic cell cycle GO:0007346 IMP
    negative regulation of cell migration GO:0030336 IDA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    HULC complex GO:0033503 IBA
    HULC complex GO:0033503 IDA
 Experiment description of studies that identified RNF20 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RNF20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRAIP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 KDF1  
Affinity Capture-MS Homo sapiens
3 HIST2H2BE 8349
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 TEX13A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 NCBP1 4686
Two-hybrid Homo sapiens
7 FAM98A 25940
Cross-Linking-MS (XL-MS) Homo sapiens
8 UBE2A  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
9 RBPMS 11030
Two-hybrid Homo sapiens
10 PMS1  
Affinity Capture-Western Homo sapiens
11 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
12 SYCE1  
Affinity Capture-MS Homo sapiens
13 NUP62 23636
Affinity Capture-MS Homo sapiens
14 ACTC1 70
Proximity Label-MS Homo sapiens
15 Chmp4b 75608
Affinity Capture-MS Mus musculus
16 C9orf78 51759
Affinity Capture-MS Homo sapiens
17 HIST1H2BB 3018
Biochemical Activity Homo sapiens
18 UBC 7316
Affinity Capture-MS Homo sapiens
19 ATM 472
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
20 RBM12 10137
Affinity Capture-MS Homo sapiens
21 IL36A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 KIAA1598 57698
Co-fractionation Homo sapiens
23 DNAJB6 10049
Affinity Capture-MS Homo sapiens
24 HIST2H2AA3 8337
Biochemical Activity Homo sapiens
25 KIAA1429 25962
Affinity Capture-MS Homo sapiens
26 CAPZB 832
Co-fractionation Homo sapiens
27 DIAPH1 1729
Co-fractionation Homo sapiens
28 GTF2E2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 C9orf16  
Affinity Capture-MS Homo sapiens
30 CIAPIN1 57019
Co-fractionation Homo sapiens
31 WHAMMP3  
Affinity Capture-MS Homo sapiens
32 TSNAX 7257
Affinity Capture-MS Homo sapiens
33 KIF5B 3799
Co-fractionation Homo sapiens
34 IMPDH2 3615
Co-fractionation Homo sapiens
35 BRCC3 79184
Co-fractionation Homo sapiens
36 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 Tsnax  
Two-hybrid Mus musculus
38 HIST2H2AC 8338
Biochemical Activity Homo sapiens
39 UCHL5 51377
Co-fractionation Homo sapiens
40 MSL2  
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
41 POLR2C 5432
Proximity Label-MS Homo sapiens
42 FAM196A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 RNF126  
Affinity Capture-MS Homo sapiens
44 HIST2H4A 8370
Biochemical Activity Homo sapiens
45 RBPJ 3516
Affinity Capture-MS Homo sapiens
46 C15orf59  
Affinity Capture-MS Homo sapiens
47 UNC45A 55898
Co-fractionation Homo sapiens
48 AMOTL2 51421
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 ZDHHC17 23390
Two-hybrid Homo sapiens
50 DIS3L  
Two-hybrid Homo sapiens
51 TCP10L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 FOXC1  
Affinity Capture-MS Homo sapiens
53 Asap1  
Two-hybrid Mus musculus
54 RBM12B  
Affinity Capture-MS Homo sapiens
55 STAU1 6780
Affinity Capture-MS Homo sapiens
56 PSMD14 10213
Affinity Capture-MS Homo sapiens
57 HTT 3064
Two-hybrid Homo sapiens
58 RPS6KB1 6198
Co-fractionation Homo sapiens
59 WAC  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 C12orf49  
Negative Genetic Homo sapiens
61 SIPA1L2  
Affinity Capture-MS Homo sapiens
62 NTRK1 4914
Affinity Capture-MS Homo sapiens
63 KIF11 3832
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
64 YWHAQ 10971
Affinity Capture-MS Homo sapiens
65 PLEKHO2 80301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CCDC101  
Affinity Capture-MS Homo sapiens
67 SLX4IP  
Affinity Capture-MS Homo sapiens
68 SUPT6H 6830
Affinity Capture-Western Homo sapiens
69 Slain1  
Affinity Capture-MS Mus musculus
70 SNAP29 9342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 DSN1 79980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 G0S2  
Affinity Capture-MS Homo sapiens
73 DISC1 27185
Affinity Capture-MS Homo sapiens
74 RNF40 9810
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
75 S100P 6286
Affinity Capture-MS Homo sapiens
76 ZSCAN4  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
77 CDC73  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
78 FAM117B  
Affinity Capture-MS Homo sapiens
79 TMPO 7112
Cross-Linking-MS (XL-MS) Homo sapiens
80 TP53BP1 7158
Negative Genetic Homo sapiens
81 TIPRL 261726
Co-fractionation Homo sapiens
82 HIST2H3C 126961
Biochemical Activity Homo sapiens
83 USP15 9958
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
84 UBE2L3 7332
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
85 CUL3 8452
Affinity Capture-MS Homo sapiens
86 UBE2E1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
87 PRMT3 10196
Co-fractionation Homo sapiens
88 RAB6B 51560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 PSMD7 5713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 Cep152  
Affinity Capture-MS Mus musculus
91 CCDC85A  
Affinity Capture-MS Homo sapiens
92 UBE2D2 7322
Reconstituted Complex Homo sapiens
93 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 SLAIN2  
Affinity Capture-MS Homo sapiens
95 PRPF19 27339
Affinity Capture-MS Homo sapiens
96 Uso1 56041
Affinity Capture-MS Mus musculus
97 Sept6  
Affinity Capture-MS Mus musculus
98 MSL1 339287
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
99 EGFR 1956
Negative Genetic Homo sapiens
100 RPL35A 6165
Affinity Capture-MS Homo sapiens
101 HSPA1A 3303
Cross-Linking-MS (XL-MS) Homo sapiens
102 USHBP1  
Two-hybrid Homo sapiens
103 TXNDC5 81567
Co-fractionation Homo sapiens
104 CSDE1 7812
Co-fractionation Homo sapiens
105 OLFM3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 AKT1 207
Co-fractionation Homo sapiens
107 LMNA 4000
Co-fractionation Homo sapiens
108 SUPT16H 11198
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
109 TPM3 7170
Affinity Capture-MS Homo sapiens
110 PA2G4 5036
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
111 LLGL2 3993
Affinity Capture-MS Homo sapiens
112 SMURF2 64750
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
113 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
114 SIRT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 RPS3 6188
Affinity Capture-MS Homo sapiens
116 TP53 7157
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
117 UBA5 79876
Affinity Capture-MS Homo sapiens
118 Ube2i  
Two-hybrid Mus musculus
119 POLR3H  
Affinity Capture-MS Homo sapiens
120 CMTR1 23070
Co-fractionation Homo sapiens
121 GBA 2629
Affinity Capture-MS Homo sapiens
122 RNF20 56254
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
123 BRK1 55845
Affinity Capture-MS Homo sapiens
124 PCNA 5111
Affinity Capture-MS Homo sapiens
125 FLNB 2317
Co-fractionation Homo sapiens
126 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
127 ITSN2 50618
Two-hybrid Homo sapiens
128 UBE2B 7320
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
129 EEF1D 1936
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
130 HSF1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
131 CDK9 1025
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
132 C1orf109  
Two-hybrid Homo sapiens
133 MYO1E 4643
Co-fractionation Homo sapiens
134 KXD1 79036
Affinity Capture-MS Homo sapiens
135 HNRNPF 3185
Affinity Capture-MS Homo sapiens
136 SERBP1 26135
Affinity Capture-MS Homo sapiens
137 KRAS 3845
Negative Genetic Homo sapiens
138 OAZ1 4946
Affinity Capture-MS Homo sapiens
139 AR 367
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
140 CCDC96  
Affinity Capture-MS Homo sapiens
141 PAF1 54623
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
142 XPNPEP1 7511
Co-fractionation Homo sapiens
143 PSMD3 5709
Co-fractionation Homo sapiens
View the network image/svg+xml



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