Gene description for RAB6B
Gene name RAB6B, member RAS oncogene family
Gene symbol RAB6B
Other names/aliases -
Species Homo sapiens
 Database cross references - RAB6B
ExoCarta ExoCarta_51560
Vesiclepedia VP_51560
Entrez Gene 51560
HGNC 14902
MIM 615852
UniProt Q9NRW1  
 RAB6B identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RAB6B
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 NAS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    myosin V binding GO:0031489 IPI
Biological Process
    intracellular protein transport GO:0006886 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 IBA
    retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum GO:0006890 NAS
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    Golgi organization GO:0007030 IMP
    neuron projection development GO:0031175 TAS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    protein localization to Golgi membrane GO:1903292 IBA
    protein localization to Golgi membrane GO:1903292 IDA
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 NAS
    cytosol GO:0005829 IEA
    endomembrane system GO:0012505 IBA
    cytoplasmic vesicle GO:0031410 IEA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified RAB6B in exosomes
1
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RAB6B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TARBP1 6894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 VASH1  
Affinity Capture-MS Homo sapiens
3 LDHA 3939
Co-fractionation Homo sapiens
4 RING1 6015
Affinity Capture-MS Homo sapiens
5 PCBP3 54039
Affinity Capture-MS Homo sapiens
6 MAVS 57506
Affinity Capture-MS Homo sapiens
7 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 RAP1GDS1 5910
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
10 RABGGTA 5875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RAB41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TGFBR2 7048
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 SLC12A4 6560
Affinity Capture-MS Homo sapiens
14 RXFP1  
Affinity Capture-MS Homo sapiens
15 METTL7A 25840
Proximity Label-MS Homo sapiens
16 OR10H2  
Affinity Capture-MS Homo sapiens
17 UQCRC2 7385
Co-fractionation Homo sapiens
18 PRNP 5621
Affinity Capture-MS Homo sapiens
19 CALR3  
Affinity Capture-MS Homo sapiens
20 RAB1B 81876
Co-fractionation Homo sapiens
21 ISOC2  
Affinity Capture-MS Homo sapiens
22 VPS13B  
Affinity Capture-MS Homo sapiens
23 GPM6A 2823
Affinity Capture-MS Homo sapiens
24 OCRL 4952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RPA2 6118
Affinity Capture-MS Homo sapiens
26 FLYWCH2  
Affinity Capture-MS Homo sapiens
27 RPA1 6117
Affinity Capture-MS Homo sapiens
28 PCDHGB5 56101
Affinity Capture-MS Homo sapiens
29 Ccdc64  
Affinity Capture-Western Mus musculus
30 CCDC117  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 KIF20A 10112
Two-hybrid Homo sapiens
32 ARF1 375
Co-fractionation Homo sapiens
33 EGFL7 51162
Affinity Capture-MS Homo sapiens
34 RAB6C 84084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CUL9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 SBF1 6305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 GDI1 2664
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 RAB33B 83452
Affinity Capture-MS Homo sapiens
39 GDI2 2665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 APEX1 328
Affinity Capture-RNA Homo sapiens
41 PCDHGB2  
Affinity Capture-MS Homo sapiens
42 COG6 57511
Two-hybrid Homo sapiens
43 SH2B2  
Two-hybrid Homo sapiens
44 PCDHA12  
Affinity Capture-MS Homo sapiens
45 RAB2A 5862
Co-fractionation Homo sapiens
46 RNF20 56254
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RPA3 6119
Affinity Capture-MS Homo sapiens
48 FAM69A 388650
Affinity Capture-MS Homo sapiens
49 SHPK 23729
Affinity Capture-MS Homo sapiens
50 TRIM21 6737
Affinity Capture-MS Homo sapiens
51 Inpp5b  
Affinity Capture-MS Mus musculus
52 TRIM31  
Affinity Capture-MS Homo sapiens
53 CHML  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 DGUOK  
Affinity Capture-MS Homo sapiens
55 ZNF131  
Affinity Capture-MS Homo sapiens
56 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CNGA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 MEOX2  
Two-hybrid Homo sapiens
59 BICD2 23299
Two-hybrid Homo sapiens
60 LYPD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 MARCH4  
Affinity Capture-MS Homo sapiens
62 ERC1 23085
Reconstituted Complex Homo sapiens
63 RAB6A 5870
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 SLC12A6 9990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 RABGGTB 5876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 TPCN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 ETFA 2108
Co-fractionation Homo sapiens
68 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 ACOX1 51
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TMPRSS11A  
Affinity Capture-MS Homo sapiens
71 MTMR1 8776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 CHM 1121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ACVR2B  
Affinity Capture-MS Homo sapiens
74 USP32 84669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 AKAP1 8165
Proximity Label-MS Homo sapiens
76 RAB1A 5861
Co-fractionation Homo sapiens
77 SCCPDH 51097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 SPCS3 60559
Affinity Capture-MS Homo sapiens
79 PCDHAC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 AQP9  
Affinity Capture-MS Homo sapiens
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