Gene description for CHM
Gene name choroideremia (Rab escort protein 1)
Gene symbol CHM
Other names/aliases DXS540
GGTA
HSD-32
REP-1
TCD
Species Homo sapiens
 Database cross references - CHM
ExoCarta ExoCarta_1121
Vesiclepedia VP_1121
Entrez Gene 1121
HGNC 1940
MIM 300390
UniProt P24386  
 CHM identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CHM
Molecular Function
    Rab geranylgeranyltransferase activity GO:0004663 TAS
    GDP-dissociation inhibitor activity GO:0005092 IEA
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IDA
    small GTPase binding GO:0031267 IPI
Biological Process
    protein targeting to membrane GO:0006612 IMP
    intracellular protein transport GO:0006886 IEA
    small GTPase-mediated signal transduction GO:0007264 IEA
    visual perception GO:0007601 IEA
    vesicle-mediated transport GO:0016192 IBA
    protein geranylgeranylation GO:0018344 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IMP
    cytosol GO:0005829 TAS
    Rab-protein geranylgeranyltransferase complex GO:0005968 IBA
    Rab-protein geranylgeranyltransferase complex GO:0005968 IDA
 Experiment description of studies that identified CHM in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CHM
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB33A 9363
Affinity Capture-MS Homo sapiens
2 RAB7B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RAB8B 51762
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TAF6 6878
Co-fractionation Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 KIF2A 3796
Co-fractionation Homo sapiens
7 RAB6B 51560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 CHML  
Negative Genetic Homo sapiens
9 VDAC3 7419
Affinity Capture-MS Homo sapiens
10 HDAC2 3066
Co-fractionation Homo sapiens
11 RABGGTA 5875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RAB1A 5861
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
13 RASEF  
Affinity Capture-MS Homo sapiens
14 TP53 7157
Affinity Capture-MS Homo sapiens
15 RAB5A 5868
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
16 RAB27B 5874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 RAB11B 9230
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 RAB17 64284
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RAB35 11021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 RAB1B 81876
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RAB29 8934
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 Rab7 19349
Affinity Capture-MS Mus musculus
23 RAB5B 5869
Affinity Capture-MS Homo sapiens
24 RAB14 51552
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RAB32 10981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 Rab5c 19345
Affinity Capture-MS Mus musculus
27 CCAR2 57805
Co-fractionation Homo sapiens
28 RAB21 23011
Affinity Capture-MS Homo sapiens
29 RAB33B 83452
Affinity Capture-MS Homo sapiens
30 RAB6A 5870
Reconstituted Complex Homo sapiens
31 DYNC1H1 1778
Affinity Capture-MS Homo sapiens
32 RAB4A 5867
Affinity Capture-MS Homo sapiens
33 RAB9B 51209
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB43 339122
Affinity Capture-MS Homo sapiens
35 RAB30 27314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 VIL1 7429
Co-fractionation Homo sapiens
37 RAB2B 84932
Affinity Capture-MS Homo sapiens
38 RBM3 5935
Affinity Capture-MS Homo sapiens
39 RAB24 53917
Affinity Capture-MS Homo sapiens
40 RAB7A 7879
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
41 RAB9A 9367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PSAP 5660
Co-fractionation Homo sapiens
43 RAB2A 5862
Proximity Label-MS Homo sapiens
44 CNTF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 RAB12 201475
Affinity Capture-MS Homo sapiens
46 RAB5C 5878
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 HSPH1 10808
Co-fractionation Homo sapiens
48 RAB3A 5864
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
49 RAB10 10890
Affinity Capture-MS Homo sapiens
50 ADRM1 11047
Co-fractionation Homo sapiens
51 RAB26  
Affinity Capture-MS Homo sapiens
52 RAB20 55647
Affinity Capture-MS Homo sapiens
53 RAB31 11031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 RAB27A 5873
Affinity Capture-MS Homo sapiens
55 RAB11A 8766
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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