Gene description for RAB2B
Gene name RAB2B, member RAS oncogene family
Gene symbol RAB2B
Other names/aliases -
Species Homo sapiens
 Database cross references - RAB2B
ExoCarta ExoCarta_84932
Vesiclepedia VP_84932
Entrez Gene 84932
HGNC 20246
MIM 607466
UniProt Q8WUD1  
 RAB2B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for RAB2B
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
Biological Process
    Golgi organization GO:0007030 IDA
    protein transport GO:0015031 IEA
    vesicle-mediated transport GO:0016192 IBA
    macroautophagy GO:0016236 IEA
    positive regulation of type I interferon production GO:0032481 ISS
    innate immune response GO:0045087 ISS
    positive regulation of exocytosis GO:0045921 IBA
    positive regulation of exocytosis GO:0045921 IMP
    defense response to virus GO:0051607 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IBA
    autophagosome membrane GO:0000421 IEA
    acrosomal vesicle GO:0001669 ISS
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified RAB2B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RAB2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 RAB21 23011
Affinity Capture-MS Homo sapiens
3 RAB18 22931
Affinity Capture-MS Homo sapiens
4 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
5 BTAF1 9044
Affinity Capture-MS Homo sapiens
6 SRSF11 9295
Two-hybrid Homo sapiens
7 CHML  
Affinity Capture-MS Homo sapiens
8 TMED10 10972
Affinity Capture-MS Homo sapiens
9 FAM71A  
Two-hybrid Homo sapiens
10 FGFR1 2260
Affinity Capture-MS Homo sapiens
11 RABGGTA 5875
Affinity Capture-MS Homo sapiens
12 RAB1A 5861
Affinity Capture-MS Homo sapiens
13 TBC1D4  
Affinity Capture-MS Homo sapiens
14 RAB5A 5868
Affinity Capture-MS Homo sapiens
15 RAB1B 81876
Affinity Capture-MS Homo sapiens
16 RAB4A 5867
Affinity Capture-MS Homo sapiens
17 DDX19B 11269
Affinity Capture-MS Homo sapiens
18 ADAMTSL4  
Two-hybrid Homo sapiens
19 DSTYK 25778
Affinity Capture-MS Homo sapiens
20 ICA1  
Two-hybrid Homo sapiens
21 BLZF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
22 UPF2 26019
Affinity Capture-MS Homo sapiens
23 TUBB8 347688
Affinity Capture-MS Homo sapiens
24 ATP12A 479
Affinity Capture-MS Homo sapiens
25 SQSTM1 8878
Proximity Label-MS Homo sapiens
26 BRD2  
Affinity Capture-MS Homo sapiens
27 FAM71C  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
29 VPS13C 54832
Affinity Capture-MS Homo sapiens
30 VRTN  
Two-hybrid Homo sapiens
31 RAB14 51552
Affinity Capture-MS Homo sapiens
32 YIPF5 81555
Affinity Capture-MS Homo sapiens
33 DDX58 23586
Affinity Capture-RNA Homo sapiens
34 STAMBPL1  
Two-hybrid Homo sapiens
35 FAM71F2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
36 APP 351
Reconstituted Complex Homo sapiens
37 GDI2 2665
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 GOLGA2 2801
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 ATF2  
Affinity Capture-MS Homo sapiens
40 NOP2 4839
Two-hybrid Homo sapiens
41 BCAS3 54828
Affinity Capture-MS Homo sapiens
42 RAB2A 5862
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 FNTA 2339
Affinity Capture-MS Homo sapiens
44 RABAC1 10567
Affinity Capture-MS Homo sapiens
45 PGGT1B 5229
Affinity Capture-MS Homo sapiens
46 PRUNE 58497
Affinity Capture-MS Homo sapiens
47 RAB9A 9367
Affinity Capture-MS Homo sapiens
48 GDI1 2664
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 FAM114A1 92689
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
50 MEOX2  
Two-hybrid Homo sapiens
51 TMEM159 57146
Affinity Capture-MS Homo sapiens
52 RPA4  
Proximity Label-MS Homo sapiens
53 PARP1 142
Proximity Label-MS Homo sapiens
54 SLC27A2 11001
Affinity Capture-MS Homo sapiens
55 UBA6 55236
Affinity Capture-MS Homo sapiens
56 FMN2  
Affinity Capture-MS Homo sapiens
57 TTF2 8458
Affinity Capture-MS Homo sapiens
58 CHM 1121
Affinity Capture-MS Homo sapiens
59 RAB5B 5869
Affinity Capture-MS Homo sapiens
60 SCARB2 950
Affinity Capture-MS Homo sapiens
61 LOC81691 81691
Affinity Capture-MS Homo sapiens
62 TBX19  
Two-hybrid Homo sapiens
63 TXNDC11 51061
Two-hybrid Homo sapiens
64 DMWD  
Affinity Capture-MS Homo sapiens
65 MTHFD2L 441024
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RAB2B is involved
PathwayEvidenceSource
Metabolism of proteins TAS Reactome
Post-translational protein modification TAS Reactome
RAB geranylgeranylation TAS Reactome





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