Gene description for PRUNE
Gene name prune exopolyphosphatase
Gene symbol PRUNE
Other names/aliases DRES-17
DRES17
HTCD37
Species Homo sapiens
 Database cross references - PRUNE
ExoCarta ExoCarta_58497
Vesiclepedia VP_58497
Entrez Gene 58497
HGNC 13420
UniProt Q86TP1  
 PRUNE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PRUNE
Molecular Function
    exopolyphosphatase activity GO:0004309 IBA
    inorganic diphosphate phosphatase activity GO:0004427 IEA
    protein binding GO:0005515 IPI
    tubulin binding GO:0015631 IDA
    phosphatase activity GO:0016791 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    regulation of microtubule polymerization GO:0031113 IMP
    regulation of neurogenesis GO:0050767 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    focal adhesion GO:0005925 IEA
 Experiment description of studies that identified PRUNE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRUNE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CXorf66  
Affinity Capture-MS Homo sapiens
2 SPANXN5  
Affinity Capture-MS Homo sapiens
3 FAM196A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 UBE2H 7328
Affinity Capture-MS Homo sapiens
5 CCL22 6367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FASN 2194
Negative Genetic Homo sapiens
7 NPAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 GMPPB 29925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RUFY3  
Affinity Capture-MS Homo sapiens
10 GSK3B 2932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SCGB1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
13 SHMT2 6472
Affinity Capture-RNA Homo sapiens
14 NXF1 10482
Affinity Capture-RNA Homo sapiens
15 GSK3A 2931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 C10orf32  
Affinity Capture-MS Homo sapiens
17 MOV10 4343
Affinity Capture-RNA Homo sapiens
18 RAB2B 84932
Affinity Capture-MS Homo sapiens
19 NME1 4830
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
20 NTRK1 4914
Affinity Capture-MS Homo sapiens
21 HSPA1A 3303
Affinity Capture-MS Homo sapiens
22 SERBP1 26135
Affinity Capture-MS Homo sapiens
23 PIANP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PLD6  
Affinity Capture-MS Homo sapiens
25 GTSF1L  
Affinity Capture-MS Homo sapiens
26 GMFG 9535
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PRUNE is involved
No pathways found





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