Gene description for MTHFD2L
Gene name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like
Gene symbol MTHFD2L
Other names/aliases -
Species Homo sapiens
 Database cross references - MTHFD2L
ExoCarta ExoCarta_441024
Vesiclepedia VP_441024
Entrez Gene 441024
HGNC 31865
MIM 614047
UniProt Q9H903  
 MTHFD2L identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for MTHFD2L
Molecular Function
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 IBA
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 ISS
    methenyltetrahydrofolate cyclohydrolase activity GO:0004477 TAS
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 ISS
    methylenetetrahydrofolate dehydrogenase (NAD+) activity GO:0004487 TAS
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 IBA
    methylenetetrahydrofolate dehydrogenase (NADP+) activity GO:0004488 ISS
    protein binding GO:0005515 IPI
Biological Process
    L-histidine biosynthetic process GO:0000105 IEA
    purine nucleotide biosynthetic process GO:0006164 IEA
    methionine biosynthetic process GO:0009086 IEA
    10-formyltetrahydrofolate metabolic process GO:0009256 IEA
    tetrahydrofolate interconversion GO:0035999 IBA
    tetrahydrofolate interconversion GO:0035999 IEA
    tetrahydrofolate interconversion GO:0035999 ISS
    folic acid metabolic process GO:0046655 TAS
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified MTHFD2L in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MTHFD2L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LAMTOR5 10542
Affinity Capture-MS Homo sapiens
2 ISCA2 122961
Affinity Capture-MS Homo sapiens
3 HDHD3 81932
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CST4 1472
Affinity Capture-MS Homo sapiens
5 HENMT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 MTHFD2 10797
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SRP9 6726
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MAPKAPK2 9261
Affinity Capture-MS Homo sapiens
9 MTL5  
Affinity Capture-MS Homo sapiens
10 BTLA  
Affinity Capture-MS Homo sapiens
11 MUCL1  
Affinity Capture-MS Homo sapiens
12 ZHX2  
Affinity Capture-MS Homo sapiens
13 RAB2B 84932
Affinity Capture-MS Homo sapiens
14 WDYHV1  
Two-hybrid Homo sapiens
15 RNASE7 84659
Affinity Capture-MS Homo sapiens
16 PROSER2 254427
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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