Experiment description of studies that identified PROSER2 in sEVs

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for PROSER2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SLC25A43   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 CSNK1G3 1456 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 PHF1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 4 KPTN   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 STK24 8428 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 6 CLIC1 1192 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 STK25 10494 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 VWA8 23078 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 GNPAT 8443 Affinity Capture-MS Homo sapiens 10 POT1   Two-hybrid Homo sapiens 11 XPO1 7514 Affinity Capture-MS Homo sapiens 12 ITFG2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 KLHL8   Affinity Capture-MS Homo sapiens 14 SNX27 81609 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 15 ARVCF 421 Affinity Capture-MS Homo sapiens 16 EZR 7430 Proximity Label-MS Homo sapiens 17 TYMS 7298 Affinity Capture-MS Homo sapiens 18 EPS8 2059 Two-hybrid Homo sapiens 19 CBWD1 55871 Affinity Capture-MS Homo sapiens 20 NELFA   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 CTU2 348180 Affinity Capture-MS Homo sapiens 22 MAPRE3   Proximity Label-MS Homo sapiens 23 STK26 51765 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 GRPEL1 80273 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 25 KAT5   Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 26 DDX58 23586 Affinity Capture-RNA Homo sapiens 27 APP 351 Reconstituted Complex Homo sapiens 28 YTHDC1   Two-hybrid Homo sapiens 29 PDCD10 11235 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 INPP5K 51763 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 31 HSPA2 3306 Affinity Capture-MS Homo sapiens 32 CLIC3 9022 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 33 UBR1 197131 Affinity Capture-MS Homo sapiens 34 RDX 5962 Proximity Label-MS Homo sapiens 35 MEMO1 51072 Affinity Capture-MS Homo sapiens 36 TYK2 7297 Affinity Capture-MS Homo sapiens 37 IPO4 79711 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 RDH13   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 DHPS 1725 Affinity Capture-MS Homo sapiens 40 IMP3 55272 Affinity Capture-MS Homo sapiens 41 ACTB 60 Two-hybrid Homo sapiens Proximity Label-MS Homo sapiens 42 ANK3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 43 SENP5   Affinity Capture-MS Homo sapiens 44 OBSL1 23363 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 45 GTPBP2 54676 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 46 GSPT1 2935 Affinity Capture-MS Homo sapiens 47 BCAT1 586 Affinity Capture-MS Homo sapiens 48 CDKN2C   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 49 MTHFD2L 441024 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which PROSER2 is involved

No pathways found