Gene description for TYK2
Gene name tyrosine kinase 2
Gene symbol TYK2
Other names/aliases IMD35
JTK1
Species Homo sapiens
 Database cross references - TYK2
ExoCarta ExoCarta_7297
Entrez Gene 7297
HGNC 12440
MIM 176941
UniProt P29597  
 TYK2 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TYK2
Molecular Function
    growth hormone receptor binding GO:0005131 IPI
    non-membrane spanning protein tyrosine kinase activity GO:0004715 IEA
    type 1 angiotensin receptor binding GO:0031702 IEA
    protein tyrosine kinase activity GO:0004713 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    cell differentiation GO:0030154 IBA
    innate immune response GO:0045087 IBA
    intracellular signal transduction GO:0035556 IEA
    cytokine-mediated signaling pathway GO:0019221 TAS
    type I interferon signaling pathway GO:0060337 TAS
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 IBA
    regulation of type I interferon-mediated signaling pathway GO:0060338 TAS
    regulation of cell proliferation GO:0042127 IBA
    cell migration GO:0016477 IBA
    protein phosphorylation GO:0006468 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    extracellular exosome GO:0070062 IDA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified TYK2 in exosomes
1
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TYK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Reconstituted Complex Homo sapiens
2 CBL 867
Affinity Capture-Western Homo sapiens
3 STAM2 10254
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
4 GHR  
Invivo Homo sapiens
5 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
6 IL6ST 3572
Invivo Homo sapiens
7 VAV1 7409
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 IFNAR1  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
9 STAT1 6772
Biochemical Activity Homo sapiens
10 FYN 2534
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
11 IRS1 3667
Invivo Homo sapiens
12 JAK1 3716
Affinity Capture-Western Homo sapiens
13 TYK2 7297
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 IL13RA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
15 CRKL 1399
Affinity Capture-Western Homo sapiens
16 PTPN1 5770
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
17 XRCC5 7520
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 PTPN6 5777
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
19 IRS2 8660
Invivo Homo sapiens
Invitro Homo sapiens
20 PRMT5 10419
Reconstituted Complex Homo sapiens
21 ELP2 55250
Affinity Capture-Western Homo sapiens
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