Gene description for TRAK1
Gene name trafficking protein, kinesin binding 1
Gene symbol TRAK1
Other names/aliases MILT1
OIP106
Species Homo sapiens
 Database cross references - TRAK1
ExoCarta ExoCarta_22906
Vesiclepedia VP_22906
Entrez Gene 22906
HGNC 29947
MIM 608112
UniProt Q9UPV9  
 TRAK1 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TRAK1
Molecular Function
    protein binding GO:0005515 IPI
    myosin binding GO:0017022 IBA
    TPR domain binding GO:0030911 IEA
    GABA receptor binding GO:0050811 IBA
Biological Process
    regulation of transcription by RNA polymerase II GO:0006357 ISS
    protein O-linked glycosylation GO:0006493 ISS
    protein targeting GO:0006605 IBA
    protein targeting GO:0006605 ISS
    endosome to lysosome transport GO:0008333 IBA
    endosome to lysosome transport GO:0008333 IDA
    neurogenesis GO:0022008 IBA
    vesicle transport along microtubule GO:0047496 IBA
    mitochondrion distribution GO:0048311 IBA
    dendrite morphogenesis GO:0048813 IEA
    positive regulation of axonogenesis GO:0050772 IEA
    anterograde axonal transport of mitochondrion GO:0098957 IBA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    early endosome GO:0005769 IDA
    cytosol GO:0005829 TAS
    cell cortex GO:0005938 IEA
    dendrite GO:0030425 IBA
    cytoplasmic vesicle GO:0031410 IBA
    mitochondrial membrane GO:0031966 IEA
    axonal growth cone GO:0044295 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified TRAK1 in sEVs
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TRAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IMMT 10989
Affinity Capture-MS Homo sapiens
2 RHOT1 55288
Affinity Capture-MS Homo sapiens
3 ENKD1  
Two-hybrid Homo sapiens
4 TRAK2 66008
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CHCHD6 84303
Affinity Capture-MS Homo sapiens
6 DGCR14  
Two-hybrid Homo sapiens
7 KLC2 64837
Affinity Capture-MS Homo sapiens
8 MTCH1 23787
Proximity Label-MS Homo sapiens
9 ZNF664  
Affinity Capture-MS Homo sapiens
10 BLOC1S6  
Two-hybrid Homo sapiens
11 SKIL  
Two-hybrid Homo sapiens
12 TRIM4 89122
Affinity Capture-MS Homo sapiens
13 GSK3B 2932
Affinity Capture-MS Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 YWHAB 7529
Affinity Capture-MS Homo sapiens
16 ZNF232  
Two-hybrid Homo sapiens
17 MTX2 10651
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 YWHAE 7531
Affinity Capture-MS Homo sapiens
19 TRAF3IP1 26146
Two-hybrid Homo sapiens
20 PRPF6 24148
Two-hybrid Homo sapiens
21 MAGEB4  
Two-hybrid Homo sapiens
22 WDR54  
Affinity Capture-MS Homo sapiens
23 MTCH2 23788
Proximity Label-MS Homo sapiens
24 POP4 10775
Affinity Capture-MS Homo sapiens
25 SAMM50 25813
Affinity Capture-MS Homo sapiens
26 BYSL 705
Two-hybrid Homo sapiens
27 RPS15 6209
Cross-Linking-MS (XL-MS) Homo sapiens
28 MYO5A 4644
Affinity Capture-MS Homo sapiens
29 MYO19  
Affinity Capture-MS Homo sapiens
30 HDAC1 3065
Affinity Capture-MS Homo sapiens
31 TRABD 80305
Affinity Capture-MS Homo sapiens
32 CDK6 1021
Biochemical Activity Homo sapiens
33 CHCHD3 54927
Affinity Capture-MS Homo sapiens
34 KLC4 89953
Affinity Capture-MS Homo sapiens
35 OCIAD1 54940
Proximity Label-MS Homo sapiens
36 GOLGA2 2801
Affinity Capture-MS Homo sapiens
37 ELP3 55140
Affinity Capture-MS Homo sapiens
38 KIF5A 3798
Affinity Capture-MS Homo sapiens
39 C9orf89 84270
Affinity Capture-MS Homo sapiens
40 YWHAZ 7534
Affinity Capture-MS Homo sapiens
41 PTBP1 5725
Two-hybrid Homo sapiens
42 APOOL 139322
Affinity Capture-MS Homo sapiens
43 TYK2 7297
Affinity Capture-MS Homo sapiens
44 OGT 8473
Reconstituted Complex Homo sapiens
45 KIF5B 3799
Affinity Capture-MS Homo sapiens
46 PLK1 5347
Affinity Capture-MS Homo sapiens
47 CEP78 84131
Affinity Capture-MS Homo sapiens
48 PEX14 5195
Affinity Capture-MS Homo sapiens
49 IFT172 26160
Affinity Capture-MS Homo sapiens
50 HGS 9146
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
51 SNW1 22938
Two-hybrid Homo sapiens
52 MTX3  
Affinity Capture-MS Homo sapiens
53 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 UTP6  
Two-hybrid Homo sapiens
55 RHOT2 89941
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SLC25A30 253512
Affinity Capture-MS Homo sapiens
57 AKAP1 8165
Proximity Label-MS Homo sapiens
58 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 CDC5L 988
Two-hybrid Homo sapiens
60 TUBB8 347688
Affinity Capture-MS Homo sapiens
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