Gene description for TRAK1
Gene name trafficking protein, kinesin binding 1
Gene symbol TRAK1
Other names/aliases MILT1
OIP106
Species Homo sapiens
 Database cross references - TRAK1
ExoCarta ExoCarta_22906
Entrez Gene 22906
HGNC 29947
MIM 608112
UniProt Q9UPV9  
 TRAK1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TRAK1
Molecular Function
    GABA receptor binding GO:0050811 IEA
    protein binding GO:0005515 IPI
Biological Process
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISS
    endosome to lysosome transport GO:0008333 IDA
    protein O-linked glycosylation GO:0006493 ISS
    protein targeting GO:0006605 ISS
Subcellular Localization
    mitochondrion GO:0005739 IDA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IDA
    early endosome GO:0005769 IDA
 Experiment description of studies that identified TRAK1 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TRAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2A 5430
Invivo Homo sapiens
2 OGT 8473
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which TRAK1 is involved
No pathways found





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