Gene description for TRIM4
Gene name tripartite motif containing 4
Gene symbol TRIM4
Other names/aliases RNF87
Species Homo sapiens
 Database cross references - TRIM4
ExoCarta ExoCarta_89122
Vesiclepedia VP_89122
Entrez Gene 89122
HGNC 16275
UniProt Q9C037  
 TRIM4 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TRIM4
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 EXP
    zinc ion binding GO:0008270 IEA
    identical protein binding GO:0042802 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    regulation of gene expression GO:0010468 IBA
    protein ubiquitination GO:0016567 IEA
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified TRIM4 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TRIM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANLN 54443
Affinity Capture-MS Homo sapiens
2 RAB39B 116442
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 KNSTRN  
Affinity Capture-MS Homo sapiens
5 TRIM4 89122
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
6 METTL14  
Affinity Capture-MS Homo sapiens
7 USP37  
Proximity Label-MS Homo sapiens
8 KIF14 9928
Affinity Capture-MS Homo sapiens
9 TRIM33 51592
Affinity Capture-MS Homo sapiens
10 ECT2 1894
Affinity Capture-MS Homo sapiens
11 CHMP4C 92421
Affinity Capture-MS Homo sapiens
12 TRIM41 90933
Two-hybrid Homo sapiens
13 CUL3 8452
Affinity Capture-MS Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 LTBR 4055
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RB1CC1 9821
Affinity Capture-MS Homo sapiens
17 Anapc2  
Affinity Capture-MS Mus musculus
18 B4GALT2  
Affinity Capture-MS Homo sapiens
19 DUSP16  
Affinity Capture-MS Homo sapiens
20 RIPK4  
Affinity Capture-MS Homo sapiens
21 KIAA1429 25962
Affinity Capture-MS Homo sapiens
22 RAB31 11031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 KDM1A 23028
Affinity Capture-Western Homo sapiens
24 CHMP4B 128866
Affinity Capture-MS Homo sapiens
25 NUBP1 4682
Affinity Capture-MS Homo sapiens
26 P4HB 5034
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PRDX1 5052
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 CIT 11113
Affinity Capture-MS Homo sapiens
29 TRIM69 140691
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PRC1 9055
Affinity Capture-MS Homo sapiens
31 ARRDC3 57561
Affinity Capture-MS Homo sapiens
32 CEBPA  
Protein-peptide Homo sapiens
33 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MDM2  
Two-hybrid Homo sapiens
35 TRAK1 22906
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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