Gene description for NUBP1
Gene name nucleotide binding protein 1
Gene symbol NUBP1
Other names/aliases NBP
NBP1
NBP35
Species Homo sapiens
 Database cross references - NUBP1
ExoCarta ExoCarta_4682
Vesiclepedia VP_4682
Entrez Gene 4682
HGNC 8041
MIM 600280
UniProt P53384  
 NUBP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
 Gene ontology annotations for NUBP1
Molecular Function
    nucleotide binding GO:0000166 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    metal ion binding GO:0046872 IEA
    iron-sulfur cluster binding GO:0051536 IBA
    iron-sulfur cluster binding GO:0051536 IDA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    ATP-dependent FeS chaperone activity GO:0140663 IEA
Biological Process
    regulation of cell growth GO:0001558 IMP
    intracellular iron ion homeostasis GO:0006879 IMP
    negative regulation of centrosome duplication GO:0010826 IEA
    iron-sulfur cluster assembly GO:0016226 IBA
    iron-sulfur cluster assembly GO:0016226 IMP
    cell projection organization GO:0030030 IEA
    centrosome localization GO:0051642 IEA
    protein localization to cell cortex GO:0072697 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    centrosome GO:0005813 IEA
    centriole GO:0005814 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    cilium GO:0005929 IEA
 Experiment description of studies that identified NUBP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NUBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ENDOG  
Affinity Capture-MS Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 HK2 3099
Co-fractionation Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 ARIH2 10425
Co-fractionation Homo sapiens
7 FNBP1L 54874
Affinity Capture-MS Homo sapiens
8 SPRY2 10253
Two-hybrid Homo sapiens
9 TRIM4 89122
Affinity Capture-MS Homo sapiens
10 PDLIM1 9124
Co-fractionation Homo sapiens
11 EHD1 10938
Co-fractionation Homo sapiens
12 SYAP1 94056
Affinity Capture-MS Homo sapiens
13 NUBP2 10101
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
14 AGAP1  
Affinity Capture-MS Homo sapiens
15 ZYX 7791
Affinity Capture-MS Homo sapiens
16 RNF41  
Affinity Capture-MS Homo sapiens
17 STK39 27347
Co-fractionation Homo sapiens
18 QPCT 25797
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 CUL3 8452
Affinity Capture-MS Homo sapiens
20 ZNF146  
Affinity Capture-MS Homo sapiens
21 ZBTB9  
Affinity Capture-MS Homo sapiens
22 FKBP9 11328
Co-fractionation Homo sapiens
23 DFFB  
Co-fractionation Homo sapiens
24 ZBTB10 65986
Affinity Capture-MS Homo sapiens
25 ADNP 23394
Affinity Capture-MS Homo sapiens
26 ZBTB21  
Affinity Capture-MS Homo sapiens
27 ZNF618  
Affinity Capture-MS Homo sapiens
28 HEXA 3073
Co-fractionation Homo sapiens
29 RB1CC1 9821
Affinity Capture-MS Homo sapiens
30 ASMTL 8623
Co-fractionation Homo sapiens
31 API5 8539
Co-fractionation Homo sapiens
32 DYNC1LI1 51143
Proximity Label-MS Homo sapiens
33 DYNC1LI2 1783
Proximity Label-MS Homo sapiens
34 DPYSL2 1808
Affinity Capture-MS Homo sapiens
35 CIAPIN1 57019
Affinity Capture-MS Homo sapiens
36 RREB1 6239
Affinity Capture-MS Homo sapiens
37 RCCD1  
Co-fractionation Homo sapiens
38 UBQLN1 29979
Co-fractionation Homo sapiens
39 XPNPEP1 7511
Co-fractionation Homo sapiens
40 KRAS 3845
Negative Genetic Homo sapiens
41 ADD1 118
Co-fractionation Homo sapiens
42 CCDC88A 55704
Affinity Capture-MS Homo sapiens
43 YBEY  
Affinity Capture-MS Homo sapiens
44 NOL3 8996
Co-fractionation Homo sapiens
45 HOOK3 84376
Affinity Capture-MS Homo sapiens
46 GNPDA1 10007
Co-fractionation Homo sapiens
47 ATP7A 538
Affinity Capture-MS Homo sapiens
48 NDN 4692
Two-hybrid Homo sapiens
49 DCTPP1 79077
Co-fractionation Homo sapiens
50 AIMP1 9255
Co-fractionation Homo sapiens
51 ZNF507  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NUBP1 is involved
PathwayEvidenceSource
Cytosolic iron-sulfur cluster assembly IEA Reactome
Metabolism IEA Reactome





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