Gene description for HOOK3
Gene name hook microtubule-tethering protein 3
Gene symbol HOOK3
Other names/aliases HK3
Species Homo sapiens
 Database cross references - HOOK3
ExoCarta ExoCarta_84376
Vesiclepedia VP_84376
Entrez Gene 84376
HGNC 23576
MIM 607825
UniProt Q86VS8  
 HOOK3 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HOOK3
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    negative regulation of neurogenesis GO:0050768 ISS
    early endosome to late endosome transport GO:0045022 IMP
    neuronal stem cell maintenance GO:0097150 IEA
    Golgi localization GO:0051645 IMP
    endosome organization GO:0007032 IMP
    endosome to lysosome transport GO:0008333 IMP
    microtubule anchoring at centrosome GO:0034454 ISS
    lysosome organization GO:0007040 IMP
    protein transport GO:0015031 IEA
    cytoplasmic microtubule organization GO:0031122 IMP
    protein localization to centrosome GO:0071539 ISS
    interkinetic nuclear migration GO:0022027 ISS
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    cytoplasm GO:0005737 IDA
    centriolar satellite GO:0034451 IEA
    FHF complex GO:0070695 IDA
    microtubule organizing center GO:0005815 IDA
    cis-Golgi network GO:0005801 IDA
    HOPS complex GO:0030897 IDA
    microtubule GO:0005874 IEA
    centrosome GO:0005813 ISS
    pericentriolar material GO:0000242 ISS
 Experiment description of studies that identified HOOK3 in exosomes
1
Experiment ID 488
MISEV standards
EM
EV Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 834
MISEV standards
EM
EV Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
EM
EV Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HOOK3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which HOOK3 is involved
No pathways found





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