Gene description for KIF1B
Gene name kinesin family member 1B
Gene symbol KIF1B
Other names/aliases CMT2
CMT2A
CMT2A1
HMSNII
KLP
NBLST1
Species Homo sapiens
 Database cross references - KIF1B
ExoCarta ExoCarta_23095
Vesiclepedia VP_23095
Entrez Gene 23095
HGNC 16636
MIM 605995
UniProt O60333  
 KIF1B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for KIF1B
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 IBA
    plus-end-directed microtubule motor activity GO:0008574 IDA
    isomerase activity GO:0016853 IEA
    ATP hydrolysis activity GO:0016887 IBA
    kinesin binding GO:0019894 TAS
Biological Process
    apoptotic process GO:0006915 IEA
    neuron-neuron synaptic transmission GO:0007270 ISS
    neuromuscular synaptic transmission GO:0007274 ISS
    vesicle-mediated transport GO:0016192 IBA
    mitochondrion transport along microtubule GO:0047497 IMP
    anterograde synaptic vesicle transport GO:0048490 ISS
    apoptotic process involved in development GO:1902742 IMP
    retrograde neuronal dense core vesicle transport GO:1990049 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 IEA
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    axon GO:0030424 IBA
    dendrite GO:0030425 IBA
    synaptic vesicle membrane GO:0030672 ISS
    cytoplasmic vesicle GO:0031410 IBA
    cytoplasmic vesicle GO:0031410 ISS
    neuron projection GO:0043005 ISS
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF1B in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KIF1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SIAH1  
Two-hybrid Homo sapiens
2 PSMA6 5687
Co-fractionation Homo sapiens
3 Tmed2 56334
Affinity Capture-MS Mus musculus
4 HOOK1  
Proximity Label-MS Homo sapiens
5 KLC2 64837
Co-fractionation Homo sapiens
6 Smc1a  
Affinity Capture-MS Mus musculus
7 KSR1  
Affinity Capture-MS Homo sapiens
8 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
9 THOC2 57187
Affinity Capture-MS Homo sapiens
10 BRCA1 672
Protein-peptide Homo sapiens
11 MAGEA1  
Affinity Capture-MS Homo sapiens
12 SRGAP2 23380
Affinity Capture-MS Homo sapiens
13 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
14 EIF4A1 1973
Affinity Capture-MS Homo sapiens
15 CALM1 801
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TRIM55  
Two-hybrid Homo sapiens
17 CBY1  
Affinity Capture-MS Homo sapiens
18 EIF4E2  
Affinity Capture-MS Homo sapiens
19 KIF13B 23303
Affinity Capture-MS Homo sapiens
20 ZBTB21  
Affinity Capture-MS Homo sapiens
21 C18orf25 147339
Co-fractionation Homo sapiens
22 SCRIB 23513
Affinity Capture-MS Homo sapiens
23 CALM3 808
Affinity Capture-MS Homo sapiens
24 TULP2  
Affinity Capture-MS Homo sapiens
25 PIK3C3 5289
Affinity Capture-MS Homo sapiens
26 CCDC6 8030
Affinity Capture-MS Homo sapiens
27 GOPC 57120
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PDF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 POLR2C 5432
Proximity Label-MS Homo sapiens
31 KIAA0368 23392
Affinity Capture-MS Homo sapiens
32 LRFN1  
Affinity Capture-MS Homo sapiens
33 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 PSMC3 5702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 HSPA8 3312
Affinity Capture-MS Homo sapiens
36 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CAV3 859
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ZNF217 7764
Affinity Capture-MS Homo sapiens
39 PIP4K2C 79837
Co-fractionation Homo sapiens
40 SMC6  
Affinity Capture-MS Homo sapiens
41 PRPH 5630
Proximity Label-MS Homo sapiens
42 RBM7  
Affinity Capture-MS Homo sapiens
43 MAPRE1 22919
Proximity Label-MS Homo sapiens
44 UBE2I 7329
Affinity Capture-MS Homo sapiens
45 RAB4A 5867
Proximity Label-MS Homo sapiens
46 PURG  
Affinity Capture-MS Homo sapiens
47 Ypel5  
Affinity Capture-MS Mus musculus
48 PTPRE 5791
Affinity Capture-MS Homo sapiens
49 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 DIRAS3  
Proximity Label-MS Homo sapiens
51 TRIM16 10626
Co-fractionation Homo sapiens
52 ACTN4 81
Co-fractionation Homo sapiens
53 ZSCAN26  
Affinity Capture-MS Homo sapiens
54 PNMA2  
Affinity Capture-MS Homo sapiens
55 DCTN1 1639
Proximity Label-MS Homo sapiens
56 PEX14 5195
Proximity Label-MS Homo sapiens
57 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 ACTB 60
Proximity Label-MS Homo sapiens
59 APBA1  
Affinity Capture-MS Homo sapiens
60 FBLN5 10516
Affinity Capture-MS Homo sapiens
61 KCTD3  
Affinity Capture-MS Homo sapiens
62 PIPSL 266971
Affinity Capture-MS Homo sapiens
63 HMGB2 3148
Co-fractionation Homo sapiens
64 PPP4R1 9989
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CETN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 ACTN1 87
Co-fractionation Homo sapiens
67 FTL 2512
Affinity Capture-MS Homo sapiens
68 VIPAS39 63894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 HOOK3 84376
Proximity Label-MS Homo sapiens
71 KIF1A 547
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 Bach1 12013
Affinity Capture-MS Mus musculus
73 SPTBN1 6711
Co-fractionation Homo sapiens
74 Mus81  
Affinity Capture-MS Mus musculus
75 MAPRE3  
Proximity Label-MS Homo sapiens
76 KCNE3  
Affinity Capture-MS Homo sapiens
77 PCBP1 5093
Affinity Capture-MS Homo sapiens
78 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 RPA3 6119
Proximity Label-MS Homo sapiens
80 STARD13 90627
Two-hybrid Homo sapiens
81 GIGYF1  
Affinity Capture-MS Homo sapiens
82 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
83 RAB9A 9367
Proximity Label-MS Homo sapiens
84 CGN  
Affinity Capture-MS Homo sapiens
85 SFN 2810
Co-fractionation Homo sapiens
86 PCM1 5108
Proximity Label-MS Homo sapiens
87 EEF1A1 1915
Two-hybrid Homo sapiens
88 TBCD 6904
Co-fractionation Homo sapiens
89 PAFAH1B1 5048
Proximity Label-MS Homo sapiens
90 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
92 SEC24A 10802
Co-fractionation Homo sapiens
93 GIPC1 10755
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
94 Tubg1 103733
Affinity Capture-MS Mus musculus
95 RTKN 6242
Affinity Capture-MS Homo sapiens
96 DENND1A 57706
Affinity Capture-MS Homo sapiens
97 VPS33B 26276
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 NINL  
Proximity Label-MS Homo sapiens
99 BIN2 51411
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 DCTN2 10540
Proximity Label-MS Homo sapiens
101 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
102 PLOD1 5351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 CAPNS1 826
Co-fractionation Homo sapiens
104 DDX58 23586
Affinity Capture-RNA Homo sapiens
105 LPXN 9404
Affinity Capture-MS Homo sapiens
106 CALM2 805
Affinity Capture-MS Homo sapiens
107 VPS26B 112936
Affinity Capture-MS Homo sapiens
108 PSMA7 5688
Co-fractionation Homo sapiens
109 KIF1C 10749
Affinity Capture-MS Homo sapiens
110 MAST3  
Affinity Capture-MS Homo sapiens
111 PARD6A  
Affinity Capture-MS Homo sapiens
112 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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