Gene description for KIF1A
Gene name kinesin family member 1A
Gene symbol KIF1A
Other names/aliases ATSV
C2orf20
HSN2C
MRD9
SPG30
UNC104
Species Homo sapiens
 Database cross references - KIF1A
ExoCarta ExoCarta_547
Vesiclepedia VP_547
Entrez Gene 547
HGNC 888
MIM 601255
UniProt Q12756  
 KIF1A identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for KIF1A
Molecular Function
    cytoskeletal motor activity GO:0003774 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 IBA
    ATP hydrolysis activity GO:0016887 IBA
    identical protein binding GO:0042802 IPI
Biological Process
    anterograde axonal transport GO:0008089 TAS
    vesicle-mediated transport GO:0016192 IBA
    regulation of dendritic spine development GO:0060998 ISS
    regulation of dendritic spine morphogenesis GO:0061001 ISS
    dense core granule cytoskeletal transport GO:0099519 ISS
    anterograde neuronal dense core vesicle transport GO:1990048 ISS
    retrograde neuronal dense core vesicle transport GO:1990049 IBA
    retrograde neuronal dense core vesicle transport GO:1990049 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    axon GO:0030424 IBA
    dendrite GO:0030425 IBA
    synapse GO:0045202 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    neuronal dense core vesicle GO:0098992 ISS
    neuronal dense core vesicle membrane GO:0099012 IEA
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF1A in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KIF1A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 CETN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NOTCH2NL 388677
Two-hybrid Homo sapiens
4 TRIP6 7205
Two-hybrid Homo sapiens
5 FBXO45 200933
Affinity Capture-MS Homo sapiens
6 MDFI  
Two-hybrid Homo sapiens
7 KIF1B 23095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 FGF11  
Affinity Capture-MS Homo sapiens
10 ANKFY1 51479
Proximity Label-MS Homo sapiens
11 PSMA3 5684
Two-hybrid Homo sapiens
12 PRKY  
Affinity Capture-MS Homo sapiens
13 RBPMS 11030
Two-hybrid Homo sapiens
14 PSMC3 5702
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RNF166  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 LAMTOR1 55004
Proximity Label-MS Homo sapiens
17 PRPS2 5634
Affinity Capture-MS Homo sapiens
18 LAMP3  
Proximity Label-MS Homo sapiens
19 DOCK5 80005
Affinity Capture-MS Homo sapiens
20 MED1 5469
Two-hybrid Homo sapiens
21 SIRT7  
Affinity Capture-MS Homo sapiens
22 ADAMTSL4  
Two-hybrid Homo sapiens
23 PPARG 5468
Two-hybrid Homo sapiens
24 SP1  
Reconstituted Complex Homo sapiens
25 MAPK8IP3  
Co-fractionation Homo sapiens
26 BZRAP1  
Two-hybrid Homo sapiens
27 SPRY2 10253
Two-hybrid Homo sapiens
28 RNF31 55072
Two-hybrid Homo sapiens
29 SPSB4  
Affinity Capture-MS Homo sapiens
30 COX15 1355
Affinity Capture-MS Homo sapiens
31 LAMP2 3920
Proximity Label-MS Homo sapiens
32 PLEKHM3 389072
Affinity Capture-MS Homo sapiens
33 PDDC1 347862
Affinity Capture-MS Homo sapiens
34 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 PARK2  
Affinity Capture-MS Homo sapiens
36 DUSP16  
Affinity Capture-MS Homo sapiens
37 RILP 83547
Affinity Capture-MS Homo sapiens
38 AARSD1 80755
Affinity Capture-MS Homo sapiens
39 MAPRE1 22919
Affinity Capture-MS Homo sapiens
40 ESR1  
Two-hybrid Homo sapiens
41 PLSCR1 5359
Two-hybrid Homo sapiens
42 KIF20A 10112
Affinity Capture-MS Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CALM2 805
Affinity Capture-MS Homo sapiens
46 KIF1BP 26128
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 DQX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 FXR2 9513
Two-hybrid Homo sapiens
49 MTUS2 23281
Two-hybrid Homo sapiens
50 NTRK1 4914
Affinity Capture-MS Homo sapiens
51 RXRA 6256
Two-hybrid Homo sapiens
52 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 RFX6  
Two-hybrid Homo sapiens
54 TRIM35 23087
Affinity Capture-MS Homo sapiens
55 TRIM27  
Two-hybrid Homo sapiens
56 KIF5B 3799
Co-fractionation Homo sapiens
57 IMP3 55272
Affinity Capture-MS Homo sapiens
58 TNS2 23371
Two-hybrid Homo sapiens
59 RAB7A 7879
Proximity Label-MS Homo sapiens
60 PTGES3 10728
Affinity Capture-MS Homo sapiens
61 CALM3 808
Affinity Capture-MS Homo sapiens
62 AR 367
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 HECTD1 25831
Affinity Capture-MS Homo sapiens
64 KIF1C 10749
Affinity Capture-MS Homo sapiens
65 RPA2 6118
Proximity Label-MS Homo sapiens
66 ENTHD2  
Affinity Capture-MS Homo sapiens
67 LYPD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TRAF1 7185
Two-hybrid Homo sapiens
69 MKRN3  
Two-hybrid Homo sapiens
70 MID2 11043
Two-hybrid Homo sapiens
71 RGS20 8601
Two-hybrid Homo sapiens
72 PIPSL 266971
Affinity Capture-MS Homo sapiens
73 RAB9A 9367
Proximity Label-MS Homo sapiens
View the network image/svg+xml



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