Gene description for PTGES3
Gene name prostaglandin E synthase 3 (cytosolic)
Gene symbol PTGES3
Other names/aliases P23
TEBP
cPGES
Species Homo sapiens
 Database cross references - PTGES3
ExoCarta ExoCarta_10728
Entrez Gene 10728
HGNC 16049
MIM 607061
UniProt Q15185  
 PTGES3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for PTGES3
Molecular Function
    prostaglandin-E synthase activity GO:0050220 IDA
    protein binding GO:0005515 IPI
    telomerase activity GO:0003720 IDA
    unfolded protein binding GO:0051082 IDA
Biological Process
    chaperone cofactor-dependent protein refolding GO:0070389 IDA
    telomere maintenance GO:0000723 TAS
    cyclooxygenase pathway GO:0019371 TAS
    prostaglandin biosynthetic process GO:0001516 IDA
    RNA-dependent DNA replication GO:0006278 IDA
    signal transduction GO:0007165 TAS
    small molecule metabolic process GO:0044281 TAS
    regulation of cellular response to heat GO:1900034 TAS
    arachidonic acid metabolic process GO:0019369 TAS
    cellular response to heat GO:0034605 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    telomerase holoenzyme complex GO:0005697 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 TAS
    chromosome, telomeric region GO:0000781 IC
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified PTGES3 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PTGES3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA4 3308
Invivo Homo sapiens
2 TERT  
Affinity Capture-Western Homo sapiens
3 ESR1  
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
4 EIF2AK2 5610
Invitro Homo sapiens
Invivo Homo sapiens
5 NR3C2  
Invivo Homo sapiens
Invitro Homo sapiens
6 DNAJB1 3337
Affinity Capture-MS Homo sapiens
7 AIP  
Invitro Homo sapiens
Invivo Homo sapiens
8 AHR 196
Invitro Homo sapiens
Invivo Homo sapiens
9 HSF1  
Reconstituted Complex Homo sapiens
10 HSP90AA1 3320
Invivo Homo sapiens
Invivo Homo sapiens
11 ERBB3 2065
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 NR3C1 2908
Invivo Homo sapiens
13 COPG2 26958
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
14 ARNT 405
Reconstituted Complex Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
15 COPG1 22820
Reconstituted Complex Homo sapiens
16 FES 2242
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which PTGES3 is involved
PathwayEvidenceSource
Attenuation phase TAS Reactome
HSF1 activation TAS Reactome
Synthesis of Prostaglandins (PG) and Thromboxanes (TX) TAS Reactome





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