Gene description for ARNT
Gene name aryl hydrocarbon receptor nuclear translocator
Gene symbol ARNT
Other names/aliases HIF-1-beta
HIF-1beta
HIF1-beta
HIF1B
HIF1BETA
TANGO
bHLHe2
Species Homo sapiens
 Database cross references - ARNT
ExoCarta ExoCarta_405
Vesiclepedia VP_405
Entrez Gene 405
HGNC 700
MIM 126110
UniProt P27540  
 ARNT identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ARNT
Molecular Function
    enhancer binding GO:0035326 IDA
    protein heterodimerization activity GO:0046982 IPI
    transcription coactivator activity GO:0003713 IEA
    RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity GO:0003705 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
    transcription factor binding GO:0008134 IPI
    sequence-specific DNA binding GO:0043565 IDA
    aryl hydrocarbon receptor activity GO:0004874 IEA
    protein binding GO:0005515 IPI
    aryl hydrocarbon receptor binding GO:0017162 IPI
Biological Process
    positive regulation of glycolytic process GO:0045821 IC
    response to hypoxia GO:0001666 IDA
    regulation of transcription from RNA polymerase II promoter in response to oxidative stress GO:0043619 IDA
    positive regulation of protein sumoylation GO:0033235 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    cellular response to hypoxia GO:0071456 TAS
    positive regulation of vascular endothelial growth factor production GO:0010575 IDA
    cell differentiation GO:0030154 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    positive regulation of erythrocyte differentiation GO:0045648 IC
    positive regulation of vascular endothelial growth factor receptor signaling pathway GO:0030949 IC
    embryonic placenta development GO:0001892 IEA
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
    intracellular receptor signaling pathway GO:0030522 IEA
    positive regulation of endothelial cell proliferation GO:0001938 IC
    mRNA transcription from RNA polymerase II promoter GO:0042789 IC
    positive regulation of hormone biosynthetic process GO:0046886 IDA
    transcription, DNA-templated GO:0006351 TAS
Subcellular Localization
    RNA polymerase II transcription factor complex GO:0090575 IDA
    cytoplasm GO:0005737 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified ARNT in exosomes
1
Experiment ID 237
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ARNT
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EPAS1  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
2 SP1  
Reconstituted Complex Homo sapiens
3 SIM2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
4 PTGES3 10728
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
5 NXF  
Two-hybrid Homo sapiens
6 SRC 6714
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 PML 5371
Invitro Homo sapiens
8 AIP 9049
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 NCOA2 10499
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
10 AHRR  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
11 GTF2F2 2963
Reconstituted Complex Homo sapiens
12 GTF2F1 2962
Reconstituted Complex Homo sapiens
13 AHR 196
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
14 SIM1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 HIF1A 3091
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which ARNT is involved
PathwayEvidenceSource
Regulation of gene expression by Hypoxia-inducible Factor TAS Reactome





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