Gene description for BCAT1
Gene name branched chain amino-acid transaminase 1, cytosolic
Gene symbol BCAT1
Other names/aliases BCATC
BCT1
ECA39
MECA39
PNAS121
PP18
Species Homo sapiens
 Database cross references - BCAT1
ExoCarta ExoCarta_586
Entrez Gene 586
HGNC 976
MIM 113520
UniProt P54687  
 BCAT1 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for BCAT1
Molecular Function
    L-isoleucine transaminase activity GO:0052656 IEA
    identical protein binding GO:0042802 IEA
    L-valine transaminase activity GO:0052655 IEA
    branched-chain-amino-acid transaminase activity GO:0004084 EXP
    L-leucine transaminase activity GO:0052654 IEA
Biological Process
    small molecule metabolic process GO:0044281 TAS
    branched-chain amino acid catabolic process GO:0009083 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    cell proliferation GO:0008283 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    branched-chain amino acid biosynthetic process GO:0009082 IEA
Subcellular Localization
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified BCAT1 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for BCAT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMAD5 4090
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which BCAT1 is involved
PathwayEvidenceSource
Branched-chain amino acid catabolism TAS Reactome





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