Gene description for SMAD5
Gene name SMAD family member 5
Gene symbol SMAD5
Other names/aliases DWFC
JV5-1
MADH5
Species Homo sapiens
 Database cross references - SMAD5
ExoCarta ExoCarta_4090
Vesiclepedia VP_4090
Entrez Gene 4090
HGNC 6771
MIM 603110
UniProt Q99717  
 SMAD5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SMAD5
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IEA
    DNA-binding transcription factor activity GO:0003700 IC
    protein binding GO:0005515 IPI
    DEAD/H-box RNA helicase binding GO:0017151 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    metal ion binding GO:0046872 IEA
    I-SMAD binding GO:0070411 IBA
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    osteoblast differentiation GO:0001649 IDA
    ureteric bud development GO:0001657 IEA
    Mullerian duct regression GO:0001880 IEA
    osteoblast fate commitment GO:0002051 IEA
    cardiac conduction system development GO:0003161 NAS
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    signal transduction GO:0007165 NAS
    transforming growth factor beta receptor signaling pathway GO:0007179 IBA
    transforming growth factor beta receptor signaling pathway GO:0007179 NAS
    germ cell development GO:0007281 IEA
    anatomical structure morphogenesis GO:0009653 IBA
    embryonic pattern specification GO:0009880 ISS
    negative regulation of gene expression GO:0010629 IMP
    cell differentiation GO:0030154 IBA
    cell differentiation GO:0030154 IDA
    erythrocyte differentiation GO:0030218 IEA
    BMP signaling pathway GO:0030509 IBA
    BMP signaling pathway GO:0030509 ISS
    BMP signaling pathway GO:0030509 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    positive regulation of osteoblast differentiation GO:0045669 IDA
    positive regulation of DNA-templated transcription GO:0045893 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 TAS
    stem cell differentiation GO:0048863 ISS
    cartilage development GO:0051216 IEA
    cardiac muscle contraction GO:0060048 IEA
    bone development GO:0060348 IEA
    SMAD protein signal transduction GO:0060395 IBA
    SMAD protein signal transduction GO:0060395 ISS
    SMAD protein signal transduction GO:0060395 NAS
    cellular response to organic cyclic compound GO:0071407 IEA
    negative regulation of Fas signaling pathway GO:1902045 IMP
Subcellular Localization
    chromatin GO:0000785 ISA
    chromatin GO:0000785 NAS
    male germ cell nucleus GO:0001673 NAS
    nucleus GO:0005634 ISS
    nucleus GO:0005634 NAS
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
    SMAD protein complex GO:0071141 NAS
    heteromeric SMAD protein complex GO:0071144 IBA
 Experiment description of studies that identified SMAD5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMAD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Affinity Capture-Western Homo sapiens
2 PHB 5245
Proximity Label-MS Homo sapiens
3 SOX7  
Two-hybrid Homo sapiens
4 KCNH2  
Affinity Capture-MS Homo sapiens
5 RUNX3  
Affinity Capture-Western Homo sapiens
6 SFPQ 6421
Two-hybrid Homo sapiens
7 TOB1  
Affinity Capture-Western Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 WBP2 23558
Two-hybrid Homo sapiens
10 SF3B1 23451
Two-hybrid Homo sapiens
11 SOX5 6660
Two-hybrid Homo sapiens
12 NEDD4 4734
Two-hybrid Homo sapiens
13 ROCK1 6093
Biochemical Activity Homo sapiens
14 SSMEM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HBG2 3048
Two-hybrid Homo sapiens
16 U2AF2 11338
Two-hybrid Homo sapiens
17 CDK8 1024
Affinity Capture-Western Homo sapiens
18 WWP1 11059
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
19 XPO1 7514
Affinity Capture-MS Homo sapiens
20 OPALIN  
Affinity Capture-MS Homo sapiens
21 NAA20  
Co-fractionation Homo sapiens
22 LSR 51599
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 EPN1 29924
Co-fractionation Homo sapiens
24 MEN1 4221
Affinity Capture-Western Homo sapiens
25 UBA1 7317
Two-hybrid Homo sapiens
26 SNRPA 6626
Two-hybrid Homo sapiens
27 SLC1A5 6510
Proximity Label-MS Homo sapiens
28 SMURF1 57154
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 TYROBP  
Affinity Capture-MS Homo sapiens
30 CHMP3 51652
Two-hybrid Homo sapiens
31 SMAD4  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
33 SKI 6497
Affinity Capture-Western Homo sapiens
34 HDAC1 3065
Affinity Capture-Western Homo sapiens
35 PLEKHO1 51177
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
36 CXXC5  
Two-hybrid Homo sapiens
37 GRPEL1 80273
Proximity Label-MS Homo sapiens
38 PNKP 11284
Two-hybrid Homo sapiens
39 DDX58 23586
Affinity Capture-RNA Homo sapiens
40 RUNX1  
Affinity Capture-Western Homo sapiens
41 ACVR1 90
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 MTMR10 54893
Two-hybrid Homo sapiens
43 PSMD11 5717
Two-hybrid Homo sapiens
44 SMAD1 4086
Two-hybrid Homo sapiens
45 RYR2 6262
Two-hybrid Homo sapiens
46 RBM4 5936
Two-hybrid Homo sapiens
47 POU6F2  
Two-hybrid Homo sapiens
48 SNRNP70 6625
Two-hybrid Homo sapiens
49 PPIL4  
Two-hybrid Homo sapiens
50 Zfp128  
Reconstituted Complex Mus musculus
Reconstituted Complex Mus musculus
51 HECW2  
Affinity Capture-MS Homo sapiens
52 PTPN12 5782
Two-hybrid Homo sapiens
53 PAX6  
Affinity Capture-Western Homo sapiens
54 CMTM5  
Affinity Capture-MS Homo sapiens
55 SUV39H2 79723
Affinity Capture-Western Homo sapiens
56 SMAD3 4088
Affinity Capture-MS Homo sapiens
57 RUNX2  
Affinity Capture-Western Homo sapiens
58 IPPK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 COPS5 10987
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
60 SMAD2 4087
Affinity Capture-MS Homo sapiens
61 ARRDC3 57561
Affinity Capture-MS Homo sapiens
62 HGS 9146
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
63 SUV39H1  
Affinity Capture-Western Homo sapiens
64 SF3B3 23450
Two-hybrid Homo sapiens
65 SMURF2 64750
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
66 HAGH 3029
Proximity Label-MS Homo sapiens
67 LEMD3  
Affinity Capture-MS Homo sapiens
68 YAP1 10413
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
69 BCAT1 586
Two-hybrid Homo sapiens
70 SMAD6  
Affinity Capture-Western Homo sapiens
71 ANKRD13A 88455
Two-hybrid Homo sapiens
72 TTYH1  
Affinity Capture-MS Homo sapiens
73 HMGA2 8091
Affinity Capture-Western Homo sapiens
74 EP300 2033
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
75 TMEM192 201931
Affinity Capture-MS Homo sapiens
76 DGCR2 9993
Affinity Capture-MS Homo sapiens
77 KAT2A  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SMAD5 is involved
PathwayEvidenceSource
Signal Transduction TAS Reactome
Signaling by BMP TAS Reactome
Signaling by TGFB family members TAS Reactome





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