Gene description for SMAD5
Gene name SMAD family member 5
Gene symbol SMAD5
Other names/aliases DWFC
JV5-1
MADH5
Species Homo sapiens
 Database cross references - SMAD5
ExoCarta ExoCarta_4090
Vesiclepedia VP_4090
Entrez Gene 4090
HGNC 6771
MIM 603110
UniProt Q99717  
 SMAD5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for SMAD5
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    receptor signaling protein activity GO:0005057 NAS
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IEA
    transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity GO:0030618 TAS
Biological Process
    bone development GO:0060348 IEA
    signal transduction GO:0007165 NAS
    positive regulation of transcription, DNA-templated GO:0045893 NAS
    cellular response to BMP stimulus GO:0071773 ISS
    erythrocyte differentiation GO:0030218 IEA
    protein phosphorylation GO:0006468 IEA
    cellular response to organic cyclic compound GO:0071407 IEA
    embryonic pattern specification GO:0009880 ISS
    Mullerian duct regression GO:0001880 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 NAS
    ureteric bud development GO:0001657 IEA
    positive regulation of osteoblast differentiation GO:0045669 IEA
    positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus GO:1901522 ISS
    BMP signaling pathway GO:0030509 TAS
    transcription, DNA-templated GO:0006351 IEA
    germ cell development GO:0007281 IEA
    cardiac muscle contraction GO:0060048 IEA
    osteoblast fate commitment GO:0002051 IEA
    intracellular signal transduction GO:0035556 TAS
    cartilage development GO:0051216 IEA
Subcellular Localization
    transcription factor complex GO:0005667 IEA
    nucleus GO:0005634 TAS
    intracellular GO:0005622 NAS
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
    integral component of membrane GO:0016021 NAS
    protein complex GO:0043234 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SMAD5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMAD5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPIL4  
Affinity Capture-MS Homo sapiens
2 ACVR1 90
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 SMAD1 4086
Affinity Capture-MS Homo sapiens
4 CHMP3 51652
Affinity Capture-MS Homo sapiens
5 RBM4 5936
Affinity Capture-MS Homo sapiens
6 SNRPA 6626
Affinity Capture-MS Homo sapiens
7 TOB1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 SMAD4  
Affinity Capture-MS Homo sapiens
9 BCAT1 586
Affinity Capture-MS Homo sapiens
10 PNKP 11284
Affinity Capture-MS Homo sapiens
11 CXXC5  
Affinity Capture-MS Homo sapiens
12 FLNA  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
13 ZNF8  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
14 U2AF2 11338
Affinity Capture-MS Homo sapiens
15 WWP1 11059
Affinity Capture-MS Homo sapiens
16 ZFHX1B  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SMAD5 is involved
PathwayEvidenceSource
Signaling by BMP TAS Reactome





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