Gene description for RBM4
Gene name RNA binding motif protein 4
Gene symbol RBM4
Other names/aliases LARK
RBM4A
ZCCHC21
ZCRB3A
Species Homo sapiens
 Database cross references - RBM4
ExoCarta ExoCarta_5936
Entrez Gene 5936
HGNC 9901
MIM 602571
UniProt Q9BWF3  
 RBM4 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for RBM4
Molecular Function
    mRNA binding GO:0003729 IDA
    RNA binding GO:0003723 IDA
    zinc ion binding GO:0008270 IEA
    nucleotide binding GO:0000166 IEA
    pre-mRNA intronic binding GO:0097157 IDA
    pre-mRNA intronic pyrimidine-rich binding GO:0097158 IDA
    protein binding GO:0005515 IPI
    miRNA binding GO:0035198 IDA
    poly(A) RNA binding GO:0044822 IDA
    mRNA 3'-UTR binding GO:0003730 ISS
Biological Process
    negative regulation of translation in response to stress GO:0032055 IDA
    cell differentiation GO:0030154 IEA
    response to arsenic-containing substance GO:0046685 IDA
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 IDA
    negative regulation of translational initiation GO:0045947 IDA
    IRES-dependent translational initiation GO:0002192 IDA
    RNA splicing GO:0008380 IEA
    negative regulation of translation involved in gene silencing by miRNA GO:0035278 IDA
    stress-activated MAPK cascade GO:0051403 IDA
    regulation of nucleocytoplasmic transport GO:0046822 IDA
    entrainment of circadian clock by photoperiod GO:0043153 ISS
    positive regulation of muscle cell differentiation GO:0051149 IDA
    negative regulation of translation GO:0017148 IDA
    circadian regulation of translation GO:0097167 ISS
    mRNA processing GO:0006397 IEA
    cap-independent translational initiation GO:0002190 IDA
    RNA processing GO:0006396 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    nucleolus GO:0005730 IDA
    cytoplasmic stress granule GO:0010494 IDA
    nuclear speck GO:0016607 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified RBM4 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RBM4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SMAD5 4090
Affinity Capture-MS Homo sapiens
2 CDK2 1017
Biochemical Activity Homo sapiens
3 CCNA1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RBM4 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here