Gene description for RBM28
Gene name RNA binding motif protein 28
Gene symbol RBM28
Other names/aliases ANES
Species Homo sapiens
 Database cross references - RBM28
ExoCarta ExoCarta_55131
Vesiclepedia VP_55131
Entrez Gene 55131
HGNC 21863
MIM 612074
UniProt Q9NW13  
 RBM28 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
 Gene ontology annotations for RBM28
Molecular Function
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
Subcellular Localization
    spliceosomal complex GO:0005681 IEA
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified RBM28 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RBM28
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 KLF6  
Proximity Label-MS Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
5 H1FNT  
Affinity Capture-MS Homo sapiens
6 RSL1D1 26156
Affinity Capture-MS Homo sapiens
7 ELF1 1997
Proximity Label-MS Homo sapiens
8 COIL  
Proximity Label-MS Homo sapiens
9 DNAJC8 22826
Proximity Label-MS Homo sapiens
10 ZNF346  
Affinity Capture-MS Homo sapiens
11 KIAA0020 9933
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 RPS17 6218
Co-fractionation Homo sapiens
13 SRPK2 6733
Affinity Capture-MS Homo sapiens
14 RSL24D1  
Affinity Capture-MS Homo sapiens
15 HIST1H1D 3007
Affinity Capture-MS Homo sapiens
16 LHX2  
Proximity Label-MS Homo sapiens
17 RPL13A 23521
Affinity Capture-MS Homo sapiens
18 RPL37A 6168
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SDAD1  
Co-fractionation Homo sapiens
20 NOP56 10528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
21 DHX8 1659
Affinity Capture-MS Homo sapiens
22 RPS19 6223
Affinity Capture-MS Homo sapiens
23 RPL10 6134
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CHCHD1  
Proximity Label-MS Homo sapiens
25 PPAN-P2RY11  
Affinity Capture-MS Homo sapiens
26 RPL14 9045
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PNMAL1  
Affinity Capture-MS Homo sapiens
28 CAND1 55832
Affinity Capture-MS Homo sapiens
29 SRP72 6731
Affinity Capture-MS Homo sapiens
30 SOX2  
Proximity Label-MS Homo sapiens
31 RPL22 6146
Affinity Capture-MS Homo sapiens
32 RPL26L1 51121
Affinity Capture-MS Homo sapiens
33 ILF2 3608
Affinity Capture-MS Homo sapiens
34 USP36  
Affinity Capture-MS Homo sapiens
35 DAXX  
Affinity Capture-MS Homo sapiens
36 DDX23 9416
Proximity Label-MS Homo sapiens
37 GPATCH4 54865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
39 HEATR3 55027
Affinity Capture-MS Homo sapiens
40 PRR11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TFAM 7019
Affinity Capture-MS Homo sapiens
42 LLPH  
Affinity Capture-MS Homo sapiens
43 KLF8  
Proximity Label-MS Homo sapiens
44 DDX24 57062
Affinity Capture-MS Homo sapiens
45 CCDC137  
Affinity Capture-MS Homo sapiens
46 RRP12 23223
Co-fractionation Homo sapiens
47 FBXW7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PRC1 9055
Affinity Capture-MS Homo sapiens
49 NPM1 4869
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ANOS1 3730
Affinity Capture-MS Homo sapiens
51 FBL 2091
Proximity Label-MS Homo sapiens
52 H2AFY2 55506
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 FGF17  
Affinity Capture-MS Homo sapiens
54 ZNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SRP14 6727
Affinity Capture-MS Homo sapiens
56 ADARB1 104
Affinity Capture-MS Homo sapiens
57 PAK1IP1  
Affinity Capture-MS Homo sapiens
58 PRKRA 8575
Affinity Capture-MS Homo sapiens
59 HIST2H2AC 8338
Affinity Capture-MS Homo sapiens
60 EED  
Affinity Capture-MS Homo sapiens
61 SRP68 6730
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 DDX18 8886
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
63 RRP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 E4F1  
Affinity Capture-MS Homo sapiens
65 RPS3A 6189
Affinity Capture-MS Homo sapiens
66 LHX4  
Proximity Label-MS Homo sapiens
67 MECP2 4204
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 CTPS1 1503
Co-fractionation Homo sapiens
69 CUL1 8454
Affinity Capture-MS Homo sapiens
70 MYCN  
Affinity Capture-MS Homo sapiens
71 RPL4 6124
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 OASL 8638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 MAK16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 OBSL1 23363
Affinity Capture-MS Homo sapiens
75 RBM8A 9939
Affinity Capture-MS Homo sapiens
76 LAMTOR1 55004
Proximity Label-MS Homo sapiens
77 PES1 23481
Co-fractionation Homo sapiens
78 LIN28A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 KLF9 687
Proximity Label-MS Homo sapiens
80 KLF16  
Proximity Label-MS Homo sapiens
81 PAPD5 64282
Affinity Capture-MS Homo sapiens
82 DHX40  
Proximity Label-MS Homo sapiens
83 KIFC1 3833
Affinity Capture-MS Homo sapiens
84 DDX31  
Affinity Capture-MS Homo sapiens
85 RPL30 6156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 POLD1 5424
Affinity Capture-MS Homo sapiens
87 NOP2 4839
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
88 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
89 DDX21 9188
Affinity Capture-MS Homo sapiens
90 TLX3  
Proximity Label-MS Homo sapiens
91 PLEKHO1 51177
Affinity Capture-MS Homo sapiens
92 TARBP2  
Affinity Capture-MS Homo sapiens
93 ZBTB47  
Affinity Capture-MS Homo sapiens
94 SOX5 6660
Proximity Label-MS Homo sapiens
95 SRSF3 6428
Affinity Capture-MS Homo sapiens
96 RPF2 84154
Affinity Capture-MS Homo sapiens
97 LYN 4067
Proximity Label-MS Homo sapiens
98 STAU1 6780
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
100 SRSF6 6431
Affinity Capture-MS Homo sapiens
101 PURG  
Affinity Capture-MS Homo sapiens
102 ZBTB48  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 POLR3F  
Co-fractionation Homo sapiens
104 RPL5 6125
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
105 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
106 RPL18A 6142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 LHX1  
Proximity Label-MS Homo sapiens
108 MTG2  
Proximity Label-MS Homo sapiens
109 NTRK1 4914
Affinity Capture-MS Homo sapiens
110 RPL31 6160
Proximity Label-MS Homo sapiens
111 IFI16 3428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 REXO4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
115 ANAPC5 51433
Affinity Capture-MS Homo sapiens
116 RBMX2  
Affinity Capture-MS Homo sapiens
117 RPL36 25873
Affinity Capture-MS Homo sapiens
118 KIF11 3832
Affinity Capture-MS Homo sapiens
119 LYAR 55646
Affinity Capture-MS Homo sapiens
120 RPS6 6194
Proximity Label-MS Homo sapiens
121 HECTD1 25831
Affinity Capture-MS Homo sapiens
122 RPLP0 6175
Affinity Capture-MS Homo sapiens
123 SRPK3  
Affinity Capture-MS Homo sapiens
124 MAGEB2 4113
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 CDX1  
Affinity Capture-MS Homo sapiens
126 MYBBP1A 10514
Affinity Capture-MS Homo sapiens
127 CEBPA  
Protein-peptide Homo sapiens
128 FGF13  
Affinity Capture-MS Homo sapiens
129 GLTSCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
130 BRCA1 672
Affinity Capture-MS Homo sapiens
131 RPA3 6119
Proximity Label-MS Homo sapiens
132 CD3EAP  
Proximity Label-MS Homo sapiens
133 WDR46  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 CNBP 7555
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 ANLN 54443
Affinity Capture-MS Homo sapiens
136 METAP2 10988
Affinity Capture-MS Homo sapiens
137 FGFBP1 9982
Affinity Capture-MS Homo sapiens
138 TLX2  
Proximity Label-MS Homo sapiens
139 KRR1 11103
Affinity Capture-MS Homo sapiens
140 ZBTB2 57621
Affinity Capture-MS Homo sapiens
141 FGF8  
Affinity Capture-MS Homo sapiens
142 MAP7 9053
Affinity Capture-MS Homo sapiens
143 MRRF  
Proximity Label-MS Homo sapiens
144 ABT1 29777
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 RPL7A 6130
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 RPL15 6138
Affinity Capture-MS Homo sapiens
147 EIF6 3692
Proximity Label-MS Homo sapiens
148 RC3H2  
Affinity Capture-MS Homo sapiens
149 SIRT7  
Affinity Capture-MS Homo sapiens
150 SRSF4 6429
Affinity Capture-MS Homo sapiens
151 ZNF622 90441
Co-fractionation Homo sapiens
152 NAT10 55226
Affinity Capture-MS Homo sapiens
153 SRSF1 6426
Affinity Capture-MS Homo sapiens
154 TAF1B  
Affinity Capture-MS Homo sapiens
155 U2AF2 11338
Affinity Capture-MS Homo sapiens
156 NPM3 10360
Affinity Capture-MS Homo sapiens
157 ZNF71  
Affinity Capture-MS Homo sapiens
158 CUL3 8452
Affinity Capture-MS Homo sapiens
159 Rrbp1  
Affinity Capture-MS Mus musculus
160 SRSF5 6430
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 RPL3 6122
Affinity Capture-MS Homo sapiens
162 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
163 YY1 7528
Proximity Label-MS Homo sapiens
164 ARF6 382
Proximity Label-MS Homo sapiens
165 SART1 9092
Affinity Capture-MS Homo sapiens
166 RPL7L1 285855
Affinity Capture-MS Homo sapiens
167 PDCD11 22984
Affinity Capture-MS Homo sapiens
168 RRP15  
Co-fractionation Homo sapiens
169 ZC3H10  
Affinity Capture-MS Homo sapiens
170 SOX15  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
171 GRSF1 2926
Proximity Label-MS Homo sapiens
172 C12orf65  
Proximity Label-MS Homo sapiens
173 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
174 DGKZ  
Affinity Capture-MS Homo sapiens
175 DKC1 1736
Co-fractionation Homo sapiens
176 POLR1E  
Proximity Label-MS Homo sapiens
177 MYC  
Affinity Capture-MS Homo sapiens
178 HECW2  
Affinity Capture-MS Homo sapiens
179 RPS13 6207
Affinity Capture-MS Homo sapiens
180 ZNF692  
Affinity Capture-MS Homo sapiens
181 DNAJC9 23234
Proximity Label-MS Homo sapiens
182 NOL12 79159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
183 RBM19 9904
Affinity Capture-MS Homo sapiens
184 RPS14 6208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
185 TOP1 7150
Co-fractionation Homo sapiens
186 PTPRB 5787
Affinity Capture-MS Homo sapiens
187 SART3 9733
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
188 DRG1 4733
Affinity Capture-MS Homo sapiens
189 RPL23A 6147
Affinity Capture-MS Homo sapiens
190 RPL18 6141
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
191 DDRGK1 65992
Affinity Capture-MS Homo sapiens
192 RNF2  
Affinity Capture-MS Homo sapiens
193 RHOB 388
Proximity Label-MS Homo sapiens
194 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
195 H2AFB2  
Affinity Capture-MS Homo sapiens
196 RPL11 6135
Affinity Capture-MS Homo sapiens
197 CCDC140  
Affinity Capture-MS Homo sapiens
198 RPL26 6154
Affinity Capture-MS Homo sapiens
199 ZC3H3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
200 RPS16 6217
Affinity Capture-MS Homo sapiens
201 RPL19 6143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
202 NIP7 51388
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
203 RPL13 6137
Affinity Capture-MS Homo sapiens
204 SURF6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
205 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
206 RC3H1 149041
Affinity Capture-MS Homo sapiens
207 SIRT6  
Affinity Capture-MS Homo sapiens
208 PARP1 142
Proximity Label-MS Homo sapiens
209 PDGFB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
210 ILF3 3609
Affinity Capture-MS Homo sapiens
211 FBXL6  
Affinity Capture-MS Homo sapiens
212 TP53 7157
Affinity Capture-MS Homo sapiens
213 RRS1 23212
Affinity Capture-MS Homo sapiens
214 RBM25 58517
Cross-Linking-MS (XL-MS) Homo sapiens
215 ETV3  
Proximity Label-MS Homo sapiens
216 MNDA 4332
Affinity Capture-MS Homo sapiens
217 ZNF746  
Affinity Capture-MS Homo sapiens
218 RNF151  
Affinity Capture-MS Homo sapiens
219 SEMA3C 10512
Affinity Capture-MS Homo sapiens
220 MEN1 4221
Affinity Capture-MS Homo sapiens
221 ZCRB1