Gene description for SRSF5
Gene name serine/arginine-rich splicing factor 5
Gene symbol SRSF5
Other names/aliases HRS
SFRS5
SRP40
Species Homo sapiens
 Database cross references - SRSF5
ExoCarta ExoCarta_6430
Vesiclepedia VP_6430
Entrez Gene 6430
HGNC 10787
MIM 600914
UniProt Q13243  
 SRSF5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Lung cancer cells 36482876    
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for SRSF5
Molecular Function
    RNA binding GO:0003723 HDA
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splice site recognition GO:0006376 TAS
    mRNA processing GO:0006397 TAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytosol GO:0005829 IDA
    nuclear speck GO:0016607 IBA
    nuclear speck GO:0016607 ISS
 Experiment description of studies that identified SRSF5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 542
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299-fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
6
Experiment ID 543
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299-fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
7
Experiment ID 544
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299-fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 545
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299-fraction 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 546
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|GAPDH
Enriched markers
✔
GOLGA2
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
10
Experiment ID 548
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
11
Experiment ID 549
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
12
Experiment ID 550
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
13
Experiment ID 551
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
14
Experiment ID 554
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
15
Experiment ID 555
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
16
Experiment ID 556
MISEV standards
✘ Biophysical techniques
✔
CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1299
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
17
Experiment ID 557
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975-fraction 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
18
Experiment ID 558
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975-fraction 4
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
19
Experiment ID 559
MISEV standards
✘ Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975-fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
20
Experiment ID 560
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
21
Experiment ID 561
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
22
Experiment ID 562
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
23
Experiment ID 563
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
24
Experiment ID 564
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
25
Experiment ID 565
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
26
Experiment ID 566
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
27
Experiment ID 567
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
28
Experiment ID 568
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36482876    
Organism Homo sapiens
Experiment description Suppression of PD-L1 release from small extracellular vesicles promotes systemic anti-tumor immunity by targeting ORAI1 calcium channels
Authors "Chen X, Li J, Zhang R, Zhang Y, Wang X, Leung EL, Ma L, Wong VKW, Liu L, Neher E, Yu H."
Journal name J Extracell Vesicles
Publication year 2022
Sample Lung cancer cells
Sample name H1975
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
29
Experiment ID 254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
30
Experiment ID 259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SRSF5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSAP58 388524
Affinity Capture-MS Homo sapiens
2 ZNF771  
Affinity Capture-MS Homo sapiens
3 ZBTB11  
Affinity Capture-MS Homo sapiens
4 ZNF346  
Affinity Capture-MS Homo sapiens
5 SRPK2 6733
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
6 MRPL24  
Affinity Capture-MS Homo sapiens
7 SEPT2 4735
Co-fractionation Homo sapiens
8 OTUD3 23252
Affinity Capture-MS Homo sapiens
9 APEX1 328
Affinity Capture-RNA Homo sapiens
10 CENPU  
Affinity Capture-MS Homo sapiens
11 RBM28 55131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 KIF23 9493
Affinity Capture-MS Homo sapiens
13 USP39 10713
Co-fractionation Homo sapiens
14 PPP3CB  
Co-fractionation Homo sapiens
15 AI837181  
Affinity Capture-MS Mus musculus
16 FLII 2314
Affinity Capture-MS Homo sapiens
17 MARCH5  
Proximity Label-MS Homo sapiens
18 RPS9 6203
Affinity Capture-MS Homo sapiens
19 RPF2 84154
Affinity Capture-MS Homo sapiens
20 PTCD1 26024
Affinity Capture-MS Homo sapiens
21 CENPQ  
Affinity Capture-MS Homo sapiens
22 MECP2 4204
Affinity Capture-MS Homo sapiens
23 ARIH2 10425
Affinity Capture-MS Homo sapiens
24 SREK1 140890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RPL19 6143
Affinity Capture-MS Homo sapiens
26 KIF14 9928
Affinity Capture-MS Homo sapiens
27 CHMP4C 92421
Affinity Capture-MS Homo sapiens
28 UTP14A 10813
Co-fractionation Homo sapiens
29 LIN28B  
Affinity Capture-MS Homo sapiens
30 MEPCE 56257
Affinity Capture-MS Homo sapiens
31 RBMX2  
Affinity Capture-MS Homo sapiens
32 HIST1H2AG 8969
Affinity Capture-MS Homo sapiens
33 SRSF3 6428
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 YBX2 51087
Affinity Capture-MS Homo sapiens
35 PURG  
Affinity Capture-MS Homo sapiens
36 HIST1H2AM 8336
Affinity Capture-MS Homo sapiens
37 RPL18A 6142
Affinity Capture-MS Homo sapiens
38 MYH9 4627
Co-fractionation Homo sapiens
39 RPL31 6160
Affinity Capture-MS Homo sapiens
40 CBX6  
Affinity Capture-MS Homo sapiens
41 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
42 HECTD1 25831
Affinity Capture-MS Homo sapiens
43 SON 6651
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
44 CDX1  
Affinity Capture-MS Homo sapiens
45 GTPBP4 23560
Affinity Capture-MS Homo sapiens
46 ANLN 54443
Affinity Capture-MS Homo sapiens
47 ABT1 29777
Affinity Capture-MS Homo sapiens
48 DAP3 7818
Affinity Capture-MS Homo sapiens
49 DOCK8 81704
Affinity Capture-MS Homo sapiens
50 KIF20A 10112
Affinity Capture-MS Homo sapiens
51 SIRT7  
Affinity Capture-MS Homo sapiens
52 SRSF4 6429
Affinity Capture-MS Homo sapiens
53 MRPL16  
Affinity Capture-MS Homo sapiens
54 NPM3 10360
Affinity Capture-MS Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 EBNA1BP2 10969
Affinity Capture-MS Homo sapiens
57 PAPD5 64282
Affinity Capture-MS Homo sapiens
58 GADD45GIP1  
Affinity Capture-MS Homo sapiens
59 RPL7L1 285855
Affinity Capture-MS Homo sapiens
60 DHX37  
Affinity Capture-MS Homo sapiens
61 NIP7 51388
Affinity Capture-MS Homo sapiens
62 ZNF512  
Affinity Capture-MS Homo sapiens
63 FASN 2194
Positive Genetic Homo sapiens
Negative Genetic Homo sapiens
64 CMSS1  
Affinity Capture-MS Homo sapiens
65 TMCO1 54499
Co-fractionation Homo sapiens
66 FBLL1 345630
Affinity Capture-MS Homo sapiens
67 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
68 ZFP62  
Affinity Capture-MS Homo sapiens
69 TMX2 51075
Co-fractionation Homo sapiens
70 RPS16 6217
Affinity Capture-MS Homo sapiens
71 U2SURP 23350
Affinity Capture-MS Homo sapiens
72 YBX1 4904
Affinity Capture-MS Homo sapiens
73 PRDM10  
Affinity Capture-MS Homo sapiens
74 RRS1 23212
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 MRPS18C  
Affinity Capture-MS Homo sapiens
76 SMN2 6607
Co-fractionation Homo sapiens
77 TRMT112