Gene description for SRSF5
Gene name serine/arginine-rich splicing factor 5
Gene symbol SRSF5
Other names/aliases HRS
SFRS5
SRP40
Species Homo sapiens
 Database cross references - SRSF5
ExoCarta ExoCarta_6430
Entrez Gene 6430
HGNC 10787
MIM 600914
UniProt Q13243  
 SRSF5 identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Melanoma cells 25950383    
 Gene ontology annotations for SRSF5
Molecular Function
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
    nucleotide binding GO:0000166 IEA
    RS domain binding GO:0050733 IEA
Biological Process
    transcription from RNA polymerase II promoter GO:0006366 TAS
    mRNA export from nucleus GO:0006406 TAS
    mRNA 3'-end processing GO:0031124 TAS
    RNA splicing GO:0008380 TAS
    mRNA splice site selection GO:0006376 TAS
    termination of RNA polymerase II transcription GO:0006369 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    mRNA processing GO:0006397 TAS
    gene expression GO:0010467 TAS
    regulation of cell cycle GO:0051726 IEA
    response to wounding GO:0009611 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
    nuclear speck GO:0016607 ISS
 Experiment description of studies that identified SRSF5 in exosomes
1
Experiment ID 254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SRSF5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SRRM1  
Invivo Homo sapiens
2 CD101 9398
Two-hybrid Homo sapiens
3 SRSF1 6426
Reconstituted Complex Homo sapiens
4 DYRK1A  
Biochemical Activity Homo sapiens
5 CECR2  
Two-hybrid Homo sapiens
6 OLFM2  
Two-hybrid Homo sapiens
View the network image/svg+xml



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