Gene description for ZNF335
Gene name zinc finger protein 335
Gene symbol ZNF335
Other names/aliases MCPH10
NIF-1
NIF1
NIF2
Species Homo sapiens
 Database cross references - ZNF335
ExoCarta ExoCarta_63925
Vesiclepedia VP_63925
Entrez Gene 63925
HGNC 15807
MIM 610827
UniProt Q9H4Z2  
 ZNF335 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ZNF335
Molecular Function
    transcription cis-regulatory region binding GO:0000976 ISS
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    histone methyltransferase binding GO:1990226 IEA
Biological Process
    in utero embryonic development GO:0001701 IEA
    positive regulation of neuroblast proliferation GO:0002052 ISS
    brain development GO:0007420 IBA
    brain development GO:0007420 IMP
    cerebral cortex neuron differentiation GO:0021895 IEA
    epigenetic regulation of gene expression GO:0040029 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    neuron projection morphogenesis GO:0048812 ISS
    brain morphogenesis GO:0048854 IEA
    positive regulation of lymphocyte proliferation GO:0050671 IMP
    positive regulation of neurogenesis GO:0050769 IBA
    positive regulation of neurogenesis GO:0050769 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IDA
    histone methyltransferase complex GO:0035097 IDA
 Experiment description of studies that identified ZNF335 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ZNF335
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPSA 3921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ASH2L 9070
Affinity Capture-Western Homo sapiens
3 NCOA6  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
4 WDR5 11091
Affinity Capture-Western Homo sapiens
5 HSPB2  
Two-hybrid Homo sapiens
6 NOTCH2 4853
Affinity Capture-MS Homo sapiens
7 CCR4 1233
Affinity Capture-Western Homo sapiens
8 HCFC1 3054
Affinity Capture-Western Homo sapiens
9 TFAP4 7023
Affinity Capture-MS Homo sapiens
10 SRSF5 6430
Affinity Capture-MS Homo sapiens
11 RPL3 6122
Affinity Capture-MS Homo sapiens
12 CCAR2 57805
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
13 KRR1 11103
Affinity Capture-MS Homo sapiens
14 C11orf30  
Affinity Capture-Western Homo sapiens
15 CDK4 1019
Biochemical Activity Homo sapiens
16 GLI4  
Affinity Capture-MS Homo sapiens
17 CDK6 1021
Biochemical Activity Homo sapiens
18 H2AFB3  
Affinity Capture-MS Homo sapiens
19 GATAD1  
Affinity Capture-MS Homo sapiens
20 PHF12  
Affinity Capture-MS Homo sapiens
21 RBBP5 5929
Affinity Capture-Western Homo sapiens
22 Rqcd1 301513
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
Two-hybrid Rattus norvegicus
View the network image/svg+xml



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