Gene description for HCFC1
Gene name host cell factor C1
Gene symbol HCFC1
Other names/aliases CFF
HCF
HCF-1
HCF1
HFC1
MRX3
PPP1R89
VCAF
Species Homo sapiens
 Database cross references - HCFC1
ExoCarta ExoCarta_3054
Entrez Gene 3054
HGNC 4839
MIM 300019
UniProt P51610  
 HCFC1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for HCFC1
Molecular Function
    identical protein binding GO:0042802 IPI
    histone acetyltransferase activity (H4-K5 specific) GO:0043995 IDA
    histone acetyltransferase activity (H4-K16 specific) GO:0046972 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001205 IEA
    transcription coactivator activity GO:0003713 IMP
    histone acetyltransferase activity (H4-K8 specific) GO:0043996 IDA
    chromatin binding GO:0003682 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 NAS
Biological Process
    release from viral latency GO:0019046 NAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    regulation of protein complex assembly GO:0043254 IDA
    histone H4-K5 acetylation GO:0043981 IDA
    mitochondrion organization GO:0007005 TAS
    organelle organization GO:0006996 TAS
    cell cycle GO:0007049 IEA
    protein stabilization GO:0050821 IDA
    positive regulation of cell cycle GO:0045787 TAS
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISS
    positive regulation of gene expression GO:0010628 IDA
    histone H4-K16 acetylation GO:0043984 IDA
    histone H4-K8 acetylation GO:0043982 IDA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    regulation of transcription, DNA-templated GO:0006355 IDA
    chromatin organization GO:0006325 TAS
Subcellular Localization
    MLL5-L complex GO:0070688 IDA
    Set1C/COMPASS complex GO:0048188 IDA
    Ada2/Gcn5/Ada3 transcription activator complex GO:0005671 IDA
    SAGA-type complex GO:0070461 IDA
    nucleus GO:0005634 IDA
    membrane GO:0016020 IDA
    cytoplasm GO:0005737 IDA
    MLL1 complex GO:0071339 IDA
    mitochondrion GO:0005739 IDA
    histone acetyltransferase complex GO:0000123 IDA
    nucleoplasm GO:0005654 TAS
    neuronal cell body GO:0043025 IDA
 Experiment description of studies that identified HCFC1 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HCFC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
2 ZBTB17  
Invivo Homo sapiens
Invitro Homo sapiens
3 WDR5 11091
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
4 HDAC2 3066
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
5 SIN3A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
6 HSPA8 3312
Affinity Capture-MS Homo sapiens
7 SP1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
8 PPP1CA 5499
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 MEN1 4221
Co-purification Homo sapiens
10 RBBP7 5931
Affinity Capture-MS Homo sapiens
11 ASCL2  
Affinity Capture-MS Homo sapiens
12 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
13 POU2F1  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
14 EGR2  
Two-hybrid Homo sapiens
15 PDCD2 5134
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
16 RBBP4 5928
Affinity Capture-MS Homo sapiens
17 ASH2L  
Reconstituted Complex Homo sapiens
18 SETD1A 9739
Affinity Capture-MS Homo sapiens
19 MLL  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 OGT 8473
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
21 ZF  
Reconstituted Complex Homo sapiens
22 HSPA4 3308
Affinity Capture-MS Homo sapiens
23 CREB3  
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
24 GABPA 2551
Invitro Homo sapiens
Two-hybrid Homo sapiens
25 PPARGC1A  
Affinity Capture-Western Homo sapiens
26 HCFC2  
Co-purification Homo sapiens
27 HCFC1R1  
Affinity Capture-Western Homo sapiens
28 SIN3B  
Affinity Capture-Western Homo sapiens
29 RBBP5  
Co-purification Homo sapiens
30 SDS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which HCFC1 is involved
PathwayEvidenceSource
HATs acetylate histones TAS Reactome
Transcriptional activation of mitochondrial biogenesis TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here