Gene description for ASH2L
Gene name ash2 (absent, small, or homeotic)-like (Drosophila)
Gene symbol ASH2L
Other names/aliases ASH2
ASH2L1
ASH2L2
Bre2
Species Homo sapiens
 Database cross references - ASH2L
ExoCarta ExoCarta_9070
Vesiclepedia VP_9070
Entrez Gene 9070
HGNC 744
MIM 604782
UniProt Q9UBL3  
 ASH2L identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for ASH2L
Molecular Function
    transcription regulatory region DNA binding GO:0044212 IDA
    metal ion binding GO:0046872 IEA
    DNA binding GO:0003677 IC
    histone methyltransferase activity (H3-K4 specific) GO:0042800 IDA
    protein binding GO:0005515 IPI
Biological Process
    transcription, DNA-templated GO:0006351 NAS
    chromatin organization GO:0006325 TAS
    hemopoiesis GO:0030097 NAS
    histone H3-K4 methylation GO:0051568 IDA
    cellular response to DNA damage stimulus GO:0006974 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    regulation of transcription, DNA-templated GO:0006355 NAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    response to estrogen GO:0043627 IDA
    positive regulation of cell proliferation GO:0008284 IMP
Subcellular Localization
    Set1C/COMPASS complex GO:0048188 IDA
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 IMP
    histone methyltransferase complex GO:0035097 IPI
 Experiment description of studies that identified ASH2L in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ASH2L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST3H3 8290
Reconstituted Complex Homo sapiens
2 ASH2L 9070
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
3 MLL  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
4 HCFC1 3054
Reconstituted Complex Homo sapiens
5 MEN1 4221
Reconstituted Complex Homo sapiens
6 DPY30 84661
Two-hybrid Homo sapiens
7 WDR5 11091
Reconstituted Complex Homo sapiens
8 RBBP5 5929
Reconstituted Complex Homo sapiens
9 HCFC2  
Reconstituted Complex Homo sapiens
10 SMAD9  
Affinity Capture-MS Homo sapiens
11 KMT2C 58508
Reconstituted Complex Homo sapiens
12 UNC119 9094
Two-hybrid Homo sapiens
View the network image/svg+xml



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