Gene description for KDM6B
Gene name lysine (K)-specific demethylase 6B
Gene symbol KDM6B
Other names/aliases JMJD3
Species Homo sapiens
 Database cross references - KDM6B
ExoCarta ExoCarta_23135
Entrez Gene 23135
HGNC 29012
MIM 611577
UniProt O15054  
 KDM6B identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for KDM6B
Molecular Function
    sequence-specific DNA binding GO:0043565 IEA
    protein binding GO:0005515 IPI
    beta-catenin binding GO:0008013 IEA
    histone demethylase activity (H3-K27 specific) GO:0071558 EXP
    dioxygenase activity GO:0051213 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISS
    cellular response to hydrogen peroxide GO:0070301 IEA
    cardiac muscle cell differentiation GO:0055007 ISS
    inflammatory response GO:0006954 IEA
    chromatin organization GO:0006325 TAS
    histone H3-K27 demethylation GO:0071557 IEA
    mesodermal cell differentiation GO:0048333 ISS
    endothelial cell differentiation GO:0045446 ISS
    oxidation-reduction process GO:0055114 IEA
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 ISS
 Experiment description of studies that identified KDM6B in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KDM6B
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which KDM6B is involved
PathwayEvidenceSource
HDMs demethylate histones TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome





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