Gene description for WAS
Gene name Wiskott-Aldrich syndrome
Gene symbol WAS
Other names/aliases IMD2
SCNX
THC
THC1
WASP
WASPA
Species Homo sapiens
 Database cross references - WAS
ExoCarta ExoCarta_7454
Entrez Gene 7454
HGNC 12731
MIM 300392
UniProt P42768  
 WAS identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for WAS
Molecular Function
    identical protein binding GO:0042802 IPI
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
    actin binding GO:0003779 IEA
    SH3 domain binding GO:0017124 IPI
    GTPase regulator activity GO:0030695 TAS
    phospholipase binding GO:0043274 IPI
Biological Process
    epidermis development GO:0008544 TAS
    regulation of catalytic activity GO:0050790 TAS
    regulation of T cell antigen processing and presentation GO:0002625 IMP
    negative regulation of cell motility GO:2000146 IMP
    actin polymerization or depolymerization GO:0008154 TAS
    endosomal transport GO:0016197 IEA
    immune response GO:0006955 IMP
    protein complex assembly GO:0006461 TAS
    actin filament-based movement GO:0030048 IEA
    actin filament polymerization GO:0030041 IEA
    innate immune response GO:0045087 TAS
    blood coagulation GO:0007596 TAS
    T cell activation GO:0042110 IEA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    T cell receptor signaling pathway GO:0050852 TAS
    defense response GO:0006952 TAS
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 IDA
    cell-cell junction GO:0005911 IEA
    actin cytoskeleton GO:0015629 TAS
    vesicle membrane GO:0012506 IEA
 Experiment description of studies that identified WAS in exosomes
1
Experiment ID 79
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
CD81|MHCII
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WAS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARPC3 10094
Two-hybrid Homo sapiens
2 FGR 2268
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 ARHGAP1 392
Affinity Capture-MS Homo sapiens
4 NCK1 4690
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
5 GRB2 2885
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 Pstpip1  
Two-hybrid Mus musculus
7 WIPF1 7456
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 ACTC1 70
Affinity Capture-MS Homo sapiens
9 EGFR 1956
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 HCK 3055
Reconstituted Complex Homo sapiens
11 VASP 7408
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 FYB 2533
Invivo Homo sapiens
13 CDC42 998
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
14 TEC 7006
Reconstituted Complex Homo sapiens
15 PSTPIP1 9051
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
16 ITSN2 50618
Invivo Homo sapiens
Two-hybrid Homo sapiens
Invitro Homo sapiens
17 FYN 2534
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
18 CRKL 1399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 LCP2 3937
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
20 TRIP10 9322
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
21 SRC 6714
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
22 ITK 3702
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
23 BTK 695
Reconstituted Complex Homo sapiens
24 RAC1 5879
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
25 PLCG1 5335
Reconstituted Complex Homo sapiens
26 PIK3R1 5295
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 RHOJ  
Invitro Homo sapiens
View the network image/svg+xml



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