Gene description for WAS
Gene name
Wiskott-Aldrich syndrome
Gene symbol
WAS
Other names/aliases
IMD2 SCNX THC THC1 WASP WASPA
Species
Homo sapiens
Database cross references - WAS
ExoCarta
ExoCarta_7454
Entrez Gene
7454
HGNC
12731
MIM
300392
UniProt
P42768
WAS identified in exosomes derived from the following tissue/cell type
B cells
20458337
B cells
20458337
B cells
20458337
Platelets
25332113
Platelets
25332113
Platelets
25332113
Gene ontology annotations for WAS
Experiment description of studies that identified WAS in exosomes
1
Experiment ID
79
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
2
Experiment ID
80
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
3
Experiment ID
81
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
4
Experiment ID
231
ISEV standards
✘
EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
5
Experiment ID
232
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
233
ISEV standards
✘
EV Biophysical techniques
✘
EV Cytosolic markers
✘
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for WAS
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
ARPC3
10094
Two-hybrid
Homo sapiens
2
FGR
2268
Invivo
Homo sapiens
Invitro
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
3
ARHGAP1
392
Affinity Capture-MS
Homo sapiens
4
NCK1
4690
Invivo
Homo sapiens
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
5
GRB2
2885
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
6
Pstpip1
Two-hybrid
Mus musculus
7
WIPF1
7456
Invitro
Homo sapiens
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
8
ACTC1
70
Affinity Capture-MS
Homo sapiens
9
EGFR
1956
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
10
HCK
3055
Reconstituted Complex
Homo sapiens
11
VASP
7408
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
12
FYB
2533
Invivo
Homo sapiens
13
CDC42
998
Invivo
Homo sapiens
Invivo
Homo sapiens
Invitro
Homo sapiens
Invitro
Homo sapiens
Affinity Capture-MS
Homo sapiens
14
TEC
7006
Reconstituted Complex
Homo sapiens
15
PSTPIP1
9051
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
16
ITSN2
50618
Invivo
Homo sapiens
Two-hybrid
Homo sapiens
Invitro
Homo sapiens
17
FYN
2534
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
18
CRKL
1399
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
19
LCP2
3937
Invitro
Homo sapiens
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
20
TRIP10
9322
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
21
SRC
6714
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
22
ITK
3702
Invivo
Homo sapiens
Invitro
Homo sapiens
Reconstituted Complex
Homo sapiens
23
BTK
695
Reconstituted Complex
Homo sapiens
24
RAC1
5879
Invivo
Homo sapiens
Affinity Capture-MS
Homo sapiens
25
PLCG1
5335
Reconstituted Complex
Homo sapiens
26
PIK3R1
5295
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
27
RHOJ
Invitro
Homo sapiens
View the network
image/svg+xml
Pathways in which WAS is involved