Gene description for FNBP1
Gene name formin binding protein 1
Gene symbol FNBP1
Other names/aliases FBP17
Species Homo sapiens
 Database cross references - FNBP1
ExoCarta ExoCarta_23048
Vesiclepedia VP_23048
Entrez Gene 23048
HGNC 17069
MIM 606191
UniProt Q96RU3  
 FNBP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for FNBP1
Molecular Function
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    identical protein binding GO:0042802 IPI
Biological Process
    endocytosis GO:0006897 IEA
    signal transduction GO:0007165 IEA
Subcellular Localization
    lysosome GO:0005764 IEA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IEA
    cell cortex GO:0005938 IEA
    cytoplasmic vesicle GO:0031410 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified FNBP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for FNBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EGF 1950
Co-localization Homo sapiens
2 FCHO1  
Co-fractionation Homo sapiens
3 ARHGAP17 55114
Reconstituted Complex Homo sapiens
4 DNM1 1759
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 RAD50 10111
Affinity Capture-MS Homo sapiens
6 FNBP1L 54874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ITSN1 6453
Two-hybrid Homo sapiens
8 MTA2 9219
Co-fractionation Homo sapiens
9 RBPJ 3516
Affinity Capture-MS Homo sapiens
10 ACOT7 11332
Affinity Capture-MS Homo sapiens
11 NR3C1 2908
Proximity Label-MS Homo sapiens
12 RIN3  
Affinity Capture-MS Homo sapiens
13 SMARCA5 8467
Co-fractionation Homo sapiens
14 DNAJA2 10294
Co-fractionation Homo sapiens
15 AKAP9 10142
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 SNX2 6643
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 FASLG 356
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 RPS19 6223
Co-fractionation Homo sapiens
19 PTEN 5728
Affinity Capture-MS Homo sapiens
20 HSPA1A 3303
Affinity Capture-MS Homo sapiens
21 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
22 TROVE2 6738
Co-fractionation Homo sapiens
23 SHMT2 6472
Affinity Capture-RNA Homo sapiens
24 EPB41L2 2037
Co-fractionation Homo sapiens
25 DDX58 23586
Affinity Capture-RNA Homo sapiens
26 ANKRD28 23243
Co-fractionation Homo sapiens
27 LYN 4067
Proximity Label-MS Homo sapiens
28 TF 7018
Co-localization Homo sapiens
29 NXF1 10482
Affinity Capture-RNA Homo sapiens
30 TNKS 8658
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
31 SF3A1 10291
Co-fractionation Homo sapiens
32 HIST2H2AA3 8337
Affinity Capture-MS Homo sapiens
33 WAS 7454
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
34 CEBPA  
Protein-peptide Homo sapiens
35 PPP1R12A 4659
Co-fractionation Homo sapiens
36 TUBB 203068
Affinity Capture-MS Homo sapiens
37 TNPO2 30000
Co-fractionation Homo sapiens
38 MYH9 4627
Co-fractionation Homo sapiens
39 GABARAP 11337
Affinity Capture-MS Homo sapiens
40 VARS 7407
Co-fractionation Homo sapiens
41 H1FX 8971
Affinity Capture-MS Homo sapiens
42 RAB11A 8766
Proximity Label-MS Homo sapiens
43 MYO1E 4643
Co-fractionation Homo sapiens
44 SPAG9 9043
Co-fractionation Homo sapiens
45 PPP2CA 5515
Co-fractionation Homo sapiens
46 ZUFSP 221302
Affinity Capture-MS Homo sapiens
47 IQGAP1 8826
Co-fractionation Homo sapiens
48 SNX27 81609
Affinity Capture-MS Homo sapiens
49 KRAS 3845
Proximity Label-MS Homo sapiens
50 FNBP1 23048
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 RHOB 388
Proximity Label-MS Homo sapiens
52 BCL6  
Affinity Capture-MS Homo sapiens
53 DNAJA1 3301
Co-fractionation Homo sapiens
54 DNM2 1785
Reconstituted Complex Homo sapiens
55 SMC2 10592
Co-fractionation Homo sapiens
56 MYH10 4628
Co-fractionation Homo sapiens
57 AGTR1  
Two-hybrid Homo sapiens
58 WDHD1  
Co-fractionation Homo sapiens
59 AHCYL1 10768
Co-fractionation Homo sapiens
60 WASL 8976
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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