Gene description for SMARCA5
Gene name
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Gene symbol
SMARCA5
Other names/aliases
ISWI SNF2H WCRF135 hISWI hSNF2H
Species
Homo sapiens
Database cross references - SMARCA5
ExoCarta
ExoCarta_8467
Entrez Gene
8467
HGNC
11101
MIM
603375
UniProt
O60264
SMARCA5 identified in exosomes derived from the following tissue/cell type
Endothelial cells
26027894
Ovarian cancer cells
23333927
Ovarian cancer cells
23333927
Thymus
23844026
Gene ontology annotations for SMARCA5
Experiment description of studies that identified SMARCA5 in exosomes
1
Experiment ID
226
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD9|FLOT1
EV Membrane markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘
EV Particle analysis
Identified molecule
mRNA
Identification method
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID
26027894
Organism
Homo sapiens
Experiment description
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name
Journal of Extracellular Vesicles
Publication year
2015
Sample
Endothelial cells
Sample name
HMEC-1
Isolation/purification methods
Differential ultracentrifugation Sucrose density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA
Methods used in the study
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting
2
Experiment ID
211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
3
Experiment ID
212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for SMARCA5
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Bmi1
Affinity Capture-MS
Mus musculus
2
SMC1A
8243
Co-purification
Homo sapiens
3
SMC3
9126
Co-purification
Homo sapiens
4
BAZ2A
Reconstituted Complex
Homo sapiens
5
Cbx2
Affinity Capture-MS
Mus musculus
6
CHRAC1
Affinity Capture-MS
Homo sapiens
Co-purification
Homo sapiens
Affinity Capture-Western
Homo sapiens
7
DNMT3B
Co-purification
Homo sapiens
8
HDAC2
3066
Co-purification
Homo sapiens
Co-purification
Homo sapiens
9
POLE3
Affinity Capture-Western
Homo sapiens
Co-purification
Homo sapiens
Affinity Capture-Western
Homo sapiens
10
CHD3
Co-purification
Homo sapiens
11
RAD21
5885
Co-purification
Homo sapiens
Co-purification
Homo sapiens
12
BAZ1B
9031
Affinity Capture-Western
Homo sapiens
13
RBBP4
5928
Co-purification
Homo sapiens
14
SATB1
6304
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
15
BAZ1A
Co-fractionation
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-purification
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-purification
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-purification
Homo sapiens
View the network
image/svg+xml
Pathways in which SMARCA5 is involved