Gene description for SMARCA5
Gene name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Gene symbol SMARCA5
Other names/aliases ISWI
SNF2H
WCRF135
hISWI
hSNF2H
Species Homo sapiens
 Database cross references - SMARCA5
ExoCarta ExoCarta_8467
Entrez Gene 8467
HGNC 11101
MIM 603375
UniProt O60264  
 SMARCA5 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for SMARCA5
Molecular Function
    helicase activity GO:0004386 IEA
    ATP binding GO:0005524 IDA
    protein binding GO:0005515 IPI
    histone binding GO:0042393 IDA
    DNA binding GO:0003677 IDA
    ATPase activity GO:0016887 IDA
    nucleosome binding GO:0031491 IEA
Biological Process
    DNA-templated transcription, initiation GO:0006352 IDA
    chromatin remodeling GO:0006338 IDA
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    ATP-dependent chromatin remodeling GO:0043044 IMP
    CENP-A containing nucleosome assembly GO:0034080 TAS
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    nucleosome assembly GO:0006334 TAS
    chromatin silencing at rDNA GO:0000183 IEA
    regulation of gene expression, epigenetic GO:0040029 TAS
    double-strand break repair GO:0006302 IEA
    gene expression GO:0010467 TAS
    nucleosome positioning GO:0016584 IDA
Subcellular Localization
    condensed chromosome GO:0000793 IDA
    NURF complex GO:0016589 IDA
    nuclear replication fork GO:0043596 IDA
    nucleoplasm GO:0005654 TAS
    RSF complex GO:0031213 IPI
    chromatin silencing complex GO:0005677 IEA
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified SMARCA5 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SMARCA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bmi1  
Affinity Capture-MS Mus musculus
2 SMC1A 8243
Co-purification Homo sapiens
3 SMC3 9126
Co-purification Homo sapiens
4 BAZ2A  
Reconstituted Complex Homo sapiens
5 Cbx2  
Affinity Capture-MS Mus musculus
6 CHRAC1  
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
7 DNMT3B  
Co-purification Homo sapiens
8 HDAC2 3066
Co-purification Homo sapiens
Co-purification Homo sapiens
9 POLE3  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
10 CHD3  
Co-purification Homo sapiens
11 RAD21 5885
Co-purification Homo sapiens
Co-purification Homo sapiens
12 BAZ1B 9031
Affinity Capture-Western Homo sapiens
13 RBBP4 5928
Co-purification Homo sapiens
14 SATB1 6304
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
15 BAZ1A  
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
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