Gene description for CTNNB1
Gene name catenin (cadherin-associated protein), beta 1, 88kDa
Gene symbol CTNNB1
Other names/aliases CTNNB
MRD19
armadillo
Species Homo sapiens
 Database cross references - CTNNB1
ExoCarta ExoCarta_1499
Entrez Gene 1499
HGNC 2514
MIM 116806
UniProt P35222  
 CTNNB1 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Colorectal cancer cells 17956143    
Colorectal cancer cells 22740476    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
 Gene ontology annotations for CTNNB1
Molecular Function
    alpha-catenin binding GO:0045294 IPI
    nitric-oxide synthase binding GO:0050998 IEA
    ion channel binding GO:0044325 IPI
    repressing transcription factor binding GO:0070491 IEA
    androgen receptor binding GO:0050681 NAS
    SMAD binding GO:0046332 IPI
    euchromatin binding GO:1990188 IDA
    ionotropic glutamate receptor binding GO:0035255 IEA
    kinase binding GO:0019900 IPI
    R-SMAD binding GO:0070412 IPI
    protein C-terminus binding GO:0008022 IPI
    transcription factor binding GO:0008134 TAS
    enzyme binding GO:0019899 IPI
    cadherin binding GO:0045296 IPI
    double-stranded DNA binding GO:0003690 IEA
    protein phosphatase binding GO:0019903 IPI
    transcription coactivator activity GO:0003713 IMP
    nuclear hormone receptor binding GO:0035257 TAS
    signal transducer activity GO:0004871 IEA
    protein binding GO:0005515 IPI
    estrogen receptor binding GO:0030331 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    transcription regulatory region DNA binding GO:0044212 IDA
    RNA polymerase II activating transcription factor binding GO:0001102 IPI
    I-SMAD binding GO:0070411 IPI
    protein complex binding GO:0032403 IEA
Biological Process
    innate immune response GO:0045087 TAS
    liver development GO:0001889 IEA
    embryonic foregut morphogenesis GO:0048617 IEA
    lung induction GO:0060492 IEA
    positive regulation of epithelial cell proliferation involved in prostate gland development GO:0060769 IEA
    negative regulation of mitotic cell cycle, embryonic GO:0045976 ISS
    embryonic hindlimb morphogenesis GO:0035116 IEA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IGI
    genitalia morphogenesis GO:0035112 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    canonical Wnt signaling pathway involved in positive regulation of cardiac outflow tract cell proliferation GO:0061324 ISS
    lens morphogenesis in camera-type eye GO:0002089 IEA
    positive regulation of type I interferon production GO:0032481 TAS
    stem cell maintenance GO:0019827 TAS
    smooth muscle cell differentiation GO:0051145 IEA
    protein heterooligomerization GO:0051291 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IEA
    T cell differentiation in thymus GO:0033077 IEA
    layer formation in cerebral cortex GO:0021819 IEA
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    embryonic heart tube development GO:0035050 IEA
    embryonic digit morphogenesis GO:0042733 IEA
    apoptotic process GO:0006915 TAS
    embryonic axis specification GO:0000578 IEA
    regulation of calcium ion import GO:0090279 IDA
    neural plate development GO:0001840 IEA
    cell fate specification GO:0001708 IEA
    mesenchymal to epithelial transition involved in metanephros morphogenesis GO:0003337 IEA
    regulation of smooth muscle cell proliferation GO:0048660 IMP
    gastrulation with mouth forming second GO:0001702 IEA
    cellular response to insulin-like growth factor stimulus GO:1990314 IEA
    cell-matrix adhesion GO:0007160 IEA
    positive regulation of heparan sulfate proteoglycan biosynthetic process GO:0010909 IMP
    single organismal cell-cell adhesion GO:0016337 IMP
    trachea formation GO:0060440 IEA
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 IEA
    epithelial tube branching involved in lung morphogenesis GO:0060441 IEA
    negative regulation of osteoclast differentiation GO:0045671 IEA
    epithelial to mesenchymal transition GO:0001837 TAS
    lung-associated mesenchyme development GO:0060484 IEA
    positive regulation of neuroblast proliferation GO:0002052 ISS
    bone resorption GO:0045453 IEA
    in utero embryonic development GO:0001701 IEA
    regulation of myelination GO:0031641 IEA
    Wnt signaling pathway GO:0016055 IDA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    thymus development GO:0048538 IEA
    osteoclast differentiation GO:0030316 IEA
    regulation of fibroblast proliferation GO:0048145 TAS
    response to cadmium ion GO:0046686 IEA
    transcription, DNA-templated GO:0006351 IEA
    renal inner medulla development GO:0072053 IEA
    regulation of cell fate specification GO:0042659 IBA
    negative regulation of cell proliferation GO:0008285 IDA
    synapse organization GO:0050808 IEA
    anterior/posterior axis specification GO:0009948 IEA
    negative regulation of oligodendrocyte differentiation GO:0048715 IEA
    regulation of centriole-centriole cohesion GO:0030997 IDA
    regulation of centromeric sister chromatid cohesion GO:0070602 IMP
    regulation of nephron tubule epithelial cell differentiation GO:0072182 ISS
    patterning of blood vessels GO:0001569 IC
    lung cell differentiation GO:0060479 IEA
    muscle cell differentiation GO:0042692 TAS
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    regulation of T cell proliferation GO:0042129 IEA
    fungiform papilla formation GO:0061198 IEA
    programmed cell death GO:0012501 TAS
    positive regulation of muscle cell differentiation GO:0051149 TAS
    regulation of secondary heart field cardioblast proliferation GO:0003266 IEA
    dorsal/ventral axis specification GO:0009950 IEA
    pancreas development GO:0031016 IEA
    hair follicle morphogenesis GO:0031069 IEA
    positive regulation of fibroblast growth factor receptor signaling pathway GO:0045743 IEA
    canonical Wnt signaling pathway GO:0060070 IDA
    glial cell fate determination GO:0007403 IEA
    renal outer medulla development GO:0072054 IEA
    Schwann cell proliferation GO:0014010 IEA
    epithelial cell differentiation involved in prostate gland development GO:0060742 IEA
    response to cytokine GO:0034097 IEA
    sympathetic ganglion development GO:0061549 ISS
    canonical Wnt signaling pathway involved in negative regulation of apoptotic process GO:0044336 IMP
    hair cell differentiation GO:0035315 TAS
    cell adhesion GO:0007155 IMP
    positive regulation of histone H3-K4 methylation GO:0051571 IC
    midgut development GO:0007494 IEA
    androgen receptor signaling pathway GO:0030521 NAS
    ectoderm development GO:0007398 IEA
    response to drug GO:0042493 IEP
    oviduct development GO:0060066 IEA
    positive regulation of DNA-templated transcription, initiation GO:2000144 IC
    cell maturation GO:0048469 IEA
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    embryonic skeletal limb joint morphogenesis GO:0036023 ISS
    negative regulation of chondrocyte differentiation GO:0032331 IEA
    regulation of protein localization to cell surface GO:2000008 IDA
    regulation of angiogenesis GO:0045765 TAS
    positive regulation of osteoblast differentiation GO:0045669 IEA
    myoblast differentiation GO:0045445 IEA
    cellular response to mechanical stimulus GO:0071260 IEA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    embryonic forelimb morphogenesis GO:0035115 IEA
    male genitalia development GO:0030539 IEA
    endodermal cell fate commitment GO:0001711 IEA
    oocyte development GO:0048599 IEA
    nephron tubule formation GO:0072079 IEA
    positive regulation of MAPK cascade GO:0043410 IEA
    response to estradiol GO:0032355 IDA
    negative regulation of neuron death GO:1901215 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    protein localization to cell surface GO:0034394 IMP
    positive regulation of determination of dorsal identity GO:2000017 IEA
    canonical Wnt signaling pathway involved in positive regulation of epithelial to mesenchymal transition GO:0044334 IMP
    hair follicle placode formation GO:0060789 IEA
    mesenchymal cell proliferation involved in lung development GO:0060916 IEA
    negative regulation of apoptotic signaling pathway GO:2001234 IEA
    adherens junction assembly GO:0034333 IMP
    cellular response to growth factor stimulus GO:0071363 IMP
    central nervous system vasculogenesis GO:0022009 IEA
    cellular response to indole-3-methanol GO:0071681 IDA
    negative regulation of protein sumoylation GO:0033234 IDA
    proximal/distal pattern formation GO:0009954 IEA
    positive regulation of apoptotic process GO:0043065 IDA
    endothelial tube morphogenesis GO:0061154 IMP
    renal vesicle formation GO:0072033 IEA
    synaptic vesicle transport GO:0048489 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    cellular component disassembly involved in execution phase of apoptosis GO:0006921 TAS
    neuron migration GO:0001764 IEA
    positive regulation of endothelial cell differentiation GO:0045603 IEA
Subcellular Localization
    cell-cell junction GO:0005911 IDA
    lateral plasma membrane GO:0016328 IDA
    Z disc GO:0030018 IEA
    beta-catenin destruction complex GO:0030877 IDA
    nucleoplasm GO:0005654 TAS
    apical part of cell GO:0045177 IEA
    cell cortex GO:0005938 IDA
    cytosol GO:0005829 TAS
    adherens junction GO:0005912 IDA
    spindle pole GO:0000922 IEA
    bicellular tight junction GO:0005923 IEA
    plasma membrane GO:0005886 IDA
    protein complex GO:0043234 IDA
    basolateral plasma membrane GO:0016323 IEA
    extracellular exosome GO:0070062 IDA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cell periphery GO:0071944 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    focal adhesion GO:0005925 IDA
    cell-cell adherens junction GO:0005913 IDA
    beta-catenin-TCF7L2 complex GO:0070369 IDA
    microvillus membrane GO:0031528 IEA
    Scrib-APC-beta-catenin complex GO:0034750 IEA
    dendritic shaft GO:0043198 IEA
    catenin complex GO:0016342 IDA
    transcription factor complex GO:0005667 IDA
    nucleus GO:0005634 IDA
    cell junction GO:0030054 TAS
    fascia adherens GO:0005916 IEA
    protein-DNA complex GO:0032993 IDA
    synapse GO:0045202 IEA
    membrane GO:0016020 ISS
    nuclear euchromatin GO:0005719 IDA
    lamellipodium GO:0030027 IEA
 Experiment description of studies that identified CTNNB1 in exosomes
1
Experiment ID 76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 20
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
CD63|CD81|LAMP1
EV Membrane markers
✔
GOLGA2|cytochrome c
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 201
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22740476    
Organism Homo sapiens
Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ.
Journal name Electrophoresis
Publication year 2012
Sample Colorectal cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 282
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
5
Experiment ID 283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 285
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 286
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 266
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Wild type
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 267
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 268
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✘ EV Membrane markers
✔
CANX|HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 254
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name MNT-1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 255
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name G1
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
13
Experiment ID 256
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 501mel
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
14
Experiment ID 257
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name Daju
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
15
Experiment ID 258
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
17
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
19
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
20
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
23
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
26
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
27
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for CTNNB1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
2 Tax1bp3  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
3 DLG5 9231
Affinity Capture-Western Homo sapiens
4 Apc2  
Affinity Capture-Western 7227
5 TCF1  
Invivo Homo sapiens
6 VEZATIN  
Affinity Capture-Western Homo sapiens