Gene description for CTNNB1
Gene name catenin (cadherin-associated protein), beta 1, 88kDa
Gene symbol CTNNB1
Other names/aliases CTNNB
MRD19
armadillo
Species Homo sapiens
 Database cross references - CTNNB1
ExoCarta ExoCarta_1499
Vesiclepedia VP_1499
Entrez Gene 1499
HGNC 2514
MIM 116806
UniProt P35222  
 CTNNB1 identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 17956143    
Colorectal cancer cells 22740476    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 34887515    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Liver cancer cells 25265333    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Melanoma cells 25950383    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Neuroblastoma cells 25944692    
Normal mammary epithelial cells 34108659    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Pluripotent stem cells 34108659    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
Saliva 19199708    
T lymphocytes 34108659    
 Gene ontology annotations for CTNNB1
Molecular Function
    transcription coregulator binding GO:0001221 ISS
    transcription corepressor binding GO:0001222 IPI
    chromatin binding GO:0003682 ISS
    transcription coactivator activity GO:0003713 IBA
    transcription coactivator activity GO:0003713 IDA
    transcription coactivator activity GO:0003713 IMP
    signaling receptor binding GO:0005102 IPI
    protein binding GO:0005515 IPI
    nuclear receptor binding GO:0016922 IBA
    nuclear receptor binding GO:0016922 IPI
    nuclear receptor binding GO:0016922 TAS
    enzyme binding GO:0019899 IPI
    kinase binding GO:0019900 IPI
    protein kinase binding GO:0019901 IEA
    protein phosphatase binding GO:0019903 IBA
    nuclear estrogen receptor binding GO:0030331 IPI
    ubiquitin protein ligase binding GO:0031625 EXP
    transmembrane transporter binding GO:0044325 IPI
    alpha-catenin binding GO:0045294 IBA
    alpha-catenin binding GO:0045294 IPI
    cadherin binding GO:0045296 HDA
    cadherin binding GO:0045296 IBA
    cadherin binding GO:0045296 IPI
    SMAD binding GO:0046332 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IDA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    I-SMAD binding GO:0070411 IPI
    disordered domain specific binding GO:0097718 IEA
    DNA-binding transcription factor binding GO:0140297 IPI
    DNA-binding transcription factor binding GO:0140297 ISS
    histone methyltransferase binding GO:1990226 ISS
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    MAPK cascade GO:0000165 IEA
    protein polyubiquitination GO:0000209 IDA
    embryonic axis specification GO:0000578 IEA
    branching involved in blood vessel morphogenesis GO:0001569 IMP
    osteoblast differentiation GO:0001649 IMP
    branching involved in ureteric bud morphogenesis GO:0001658 IEA
    in utero embryonic development GO:0001701 IEA
    gastrulation with mouth forming second GO:0001702 IEA
    cell fate specification GO:0001708 IEA
    endodermal cell fate commitment GO:0001711 IEA
    neuron migration GO:0001764 IEA
    epithelial to mesenchymal transition GO:0001837 TAS
    neural plate development GO:0001840 IEA
    positive regulation of neuroblast proliferation GO:0002052 ISS
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    chondrocyte differentiation GO:0002062 IEA
    lens morphogenesis in camera-type eye GO:0002089 IEA
    outflow tract morphogenesis GO:0003151 ISS
    regulation of secondary heart field cardioblast proliferation GO:0003266 IEA
    metanephros morphogenesis GO:0003338 IEA
    negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis GO:0003340 IEA
    transcription by RNA polymerase II GO:0006366 IEA
    cell adhesion GO:0007155 IMP
    cell-matrix adhesion GO:0007160 IEA
    chemical synaptic transmission GO:0007268 IEA
    ectoderm development GO:0007398 IEA
    glial cell fate determination GO:0007403 IEA
    neuroblast proliferation GO:0007405 IEA
    positive regulation of cell population proliferation GO:0008284 ISS
    negative regulation of cell population proliferation GO:0008285 IDA
    fibroblast growth factor receptor signaling pathway GO:0008543 IEA
    response to xenobiotic stimulus GO:0009410 IEP
    anterior/posterior axis specification GO:0009948 IEA
    dorsal/ventral axis specification GO:0009950 IEA
    proximal/distal pattern formation GO:0009954 IEA
    positive regulation of gene expression GO:0010628 IEA
    negative regulation of gene expression GO:0010629 IEA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IGI
    positive regulation of epithelial to mesenchymal transition GO:0010718 IMP
    positive regulation of heparan sulfate proteoglycan biosynthetic process GO:0010909 IMP
    negative regulation of angiogenesis GO:0016525 ISS
    stem cell population maintenance GO:0019827 TAS
    layer formation in cerebral cortex GO:0021819 IEA
    hypothalamus development GO:0021854 IEA
    central nervous system vasculogenesis GO:0022009 IEA
    osteoclast differentiation GO:0030316 IEA
    male genitalia development GO:0030539 IEA
    hindbrain development GO:0030902 IEA
    regulation of centriole-centriole cohesion GO:0030997 IDA
    pancreas development GO:0031016 IEA
    hair follicle morphogenesis GO:0031069 IEA
    regulation of protein ubiquitination GO:0031396 IMP
    regulation of myelination GO:0031641 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISS
    negative regulation of chondrocyte differentiation GO:0032331 IEA
    response to estradiol GO:0032355 IDA
    positive regulation of transcription elongation by RNA polymerase II GO:0032968 IEA
    T cell differentiation in thymus GO:0033077 IEA
    negative regulation of protein sumoylation GO:0033234 IDA
    adherens junction assembly GO:0034333 IMP
    protein localization to cell surface GO:0034394 IMP
    embryonic heart tube development GO:0035050 IEA
    genitalia morphogenesis GO:0035112 IEA
    embryonic forelimb morphogenesis GO:0035115 IEA
    embryonic hindlimb morphogenesis GO:0035116 IEA
    hair cell differentiation GO:0035315 TAS
    detection of muscle stretch GO:0035995 TAS
    embryonic skeletal limb joint morphogenesis GO:0036023 ISS
    astrocyte-dopaminergic neuron signaling GO:0036520 IEA
    regulation of T cell proliferation GO:0042129 IEA
    odontogenesis of dentin-containing tooth GO:0042475 IEA
    embryonic digit morphogenesis GO:0042733 IEA
    positive regulation of apoptotic process GO:0043065 IDA
    negative regulation of apoptotic process GO:0043066 IMP
    negative regulation of apoptotic process GO:0043066 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of neuron apoptotic process GO:0043525 IDA
    cell-cell adhesion mediated by cadherin GO:0044331 IMP
    canonical Wnt signaling pathway involved in mesenchymal stem cell differentiation GO:0044338 IEA
    bone resorption GO:0045453 IEA
    positive regulation of cell differentiation GO:0045597 NAS
    positive regulation of endothelial cell differentiation GO:0045603 IEA
    positive regulation of osteoblast differentiation GO:0045669 IEA
    negative regulation of osteoclast differentiation GO:0045671 IEA
    positive regulation of fibroblast growth factor receptor signaling pathway GO:0045743 IEA
    regulation of angiogenesis GO:0045765 TAS
    negative regulation of DNA-templated transcription GO:0045892 IMP
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 TAS
    negative regulation of mitotic cell cycle, embryonic GO:0045976 ISS
    regulation of fibroblast proliferation GO:0048145 TAS
    cell maturation GO:0048469 IEA
    synaptic vesicle transport GO:0048489 IEA
    thymus development GO:0048538 IEA
    oocyte development GO:0048599 IEA
    embryonic foregut morphogenesis GO:0048617 IEA
    positive regulation of skeletal muscle tissue development GO:0048643 IEA
    regulation of smooth muscle cell proliferation GO:0048660 IMP
    neuron fate determination GO:0048664 IEA
    oligodendrocyte differentiation GO:0048709 IEA
    negative regulation of oligodendrocyte differentiation GO:0048715 IEA
    regulation of neurogenesis GO:0050767 NAS
    regulation of neurogenesis GO:0050767 TAS
    synapse organization GO:0050808 IEA
    smooth muscle cell differentiation GO:0051145 IEA
    myoblast proliferation GO:0051450 IEA
    regulation of timing of anagen GO:0051884 IEA
    regulation of synapse assembly GO:0051963 IEA
    oviduct development GO:0060066 IEA
    canonical Wnt signaling pathway GO:0060070 IBA
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 IGI
    canonical Wnt signaling pathway GO:0060070 IMP
    canonical Wnt signaling pathway GO:0060070 ISS
    canonical Wnt signaling pathway GO:0060070 TAS
    trachea formation GO:0060440 IEA
    epithelial tube branching involved in lung morphogenesis GO:0060441 IEA
    lung-associated mesenchyme development GO:0060484 IEA
    lung epithelial cell differentiation GO:0060487 IEA
    lung induction GO:0060492 IEA
    epithelial cell differentiation involved in prostate gland development GO:0060742 IEA
    epithelial cell proliferation involved in prostate gland development GO:0060767 IEA
    positive regulation of epithelial cell proliferation involved in prostate gland development GO:0060769 IEA
    hair follicle placode formation GO:0060789 IEA
    establishment of blood-brain barrier GO:0060856 IEA
    mesenchymal cell proliferation involved in lung development GO:0060916 IEA
    positive regulation of branching involved in lung morphogenesis GO:0061047 IEA
    endothelial tube morphogenesis GO:0061154 IMP
    fungiform papilla formation GO:0061198 IEA
    sympathetic ganglion development GO:0061549 ISS
    cranial ganglion development GO:0061550 IEA
    regulation of centromeric sister chromatid cohesion GO:0070602 IMP
    cellular response to growth factor stimulus GO:0071363 IMP
    cellular response to indole-3-methanol GO:0071681 IDA
    renal vesicle formation GO:0072033 IEA
    renal inner medulla development GO:0072053 IEA
    renal outer medulla development GO:0072054 IEA
    nephron tubule formation GO:0072079 IEA
    stem cell proliferation GO:0072089 IEA
    regulation of nephron tubule epithelial cell differentiation GO:0072182 ISS
    mesenchymal stem cell differentiation GO:0072497 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    regulation of calcium ion import GO:0090279 IDA
    acinar cell differentiation GO:0090425 IEA
    synaptic vesicle clustering GO:0097091 IEA
    apoptotic signaling pathway GO:0097190 IEA
    cell-cell adhesion GO:0098609 IBA
    positive regulation of odontoblast differentiation GO:1901331 IEA
    negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903377 IEA
    cranial skeletal system development GO:1904888 IEA
    midbrain dopaminergic neuron differentiation GO:1904948 ISS
    neuron projection extension GO:1990138 IMP
    embryonic brain development GO:1990403 IEA
    dorsal root ganglion development GO:1990791 IEA
    establishment of blood-retinal barrier GO:1990963 IEA
    regulation of protein localization to cell surface GO:2000008 IDA
    positive regulation of determination of dorsal identity GO:2000017 IEA
    positive regulation of myoblast proliferation GO:2000288 IEA
    positive regulation of stem cell proliferation GO:2000648 IEA
Subcellular Localization
    euchromatin GO:0000791 IDA
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    transcription regulator complex GO:0005667 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell-cell junction GO:0005911 IDA
    adherens junction GO:0005912 IBA
    adherens junction GO:0005912 IDA
    adherens junction GO:0005912 IMP
    fascia adherens GO:0005916 IEA
    bicellular tight junction GO:0005923 IEA
    focal adhesion GO:0005925 HDA
    cell cortex GO:0005938 IDA
    membrane GO:0016020 ISS
    basolateral plasma membrane GO:0016323 IDA
    apicolateral plasma membrane GO:0016327 IEA
    lateral plasma membrane GO:0016328 IDA
    catenin complex GO:0016342 IBA
    catenin complex GO:0016342 IDA
    flotillin complex GO:0016600 IEA
    Z disc GO:0030018 IEA
    lamellipodium GO:0030027 IEA
    cell junction GO:0030054 IDA
    cell junction GO:0030054 TAS
    beta-catenin destruction complex GO:0030877 IDA
    microvillus membrane GO:0031528 IEA
    protein-containing complex GO:0032991 IDA
    protein-DNA complex GO:0032993 IDA
    Scrib-APC-beta-catenin complex GO:0034750 IEA
    presynaptic membrane GO:0042734 IEA
    apical part of cell GO:0045177 IEA
    synapse GO:0045202 ISS
    postsynaptic membrane GO:0045211 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
    beta-catenin-TCF7L2 complex GO:0070369 IDA
    cell periphery GO:0071944 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    presynaptic active zone cytoplasmic component GO:0098831 IEA
    glutamatergic synapse GO:0098978 IEA
    postsynaptic density, intracellular component GO:0099092 IEA
    beta-catenin-ICAT complex GO:1990711 IEA
    beta-catenin-TCF complex GO:1990907 IDA
    beta-catenin-TCF complex GO:1990907 IPI
    Wnt signalosome GO:1990909 NAS
 Experiment description of studies that identified CTNNB1 in exosomes
1
Experiment ID 76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors "Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
Western blotting
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
13
Experiment ID 201
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22740476    
Organism Homo sapiens
Experiment description Restoration of full-length APC protein in SW480 colon cancer cells induces exosome-mediated secretion of DKK-4.
Authors "Lim JW, Mathias RA, Kapp EA, Layton MJ, Faux MC, Burgess AW, Ji H, Simpson RJ."
Journal name Electrophoresis
Publication year 2012
Sample Colorectal cancer cells
Sample name SW480
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 282
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 283
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
16
Experiment ID 285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
17
Experiment ID 286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘ Negative markers
✔
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
18
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
19
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
21
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
22
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
23
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
25
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
26
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."