Gene description for PTPN13
Gene name protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase)
Gene symbol PTPN13
Other names/aliases FAP-1
PNP1
PTP-BAS
PTP-BL
PTP1E
PTPL1
PTPLE
hPTP1E
Species Homo sapiens
 Database cross references - PTPN13
ExoCarta ExoCarta_5783
Vesiclepedia VP_5783
Entrez Gene 5783
HGNC 9646
MIM 600267
UniProt Q12923  
 PTPN13 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 19056867    
 Gene ontology annotations for PTPN13
Molecular Function
    protein tyrosine phosphatase activity GO:0004725 IBA
    protein tyrosine phosphatase activity GO:0004725 IDA
    protein tyrosine phosphatase activity GO:0004725 IMP
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    phosphatidylinositol 3-kinase regulatory subunit binding GO:0036312 IBA
    phosphatidylinositol 3-kinase regulatory subunit binding GO:0036312 IPI
    histone H2AXY142 phosphatase activity GO:0140793 IEA
Biological Process
    negative regulation of protein phosphorylation GO:0001933 IMP
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 IDA
    peptidyl-tyrosine dephosphorylation GO:0035335 IMP
    regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051896 IBA
    regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051896 IDA
    cellular response to toxic substance GO:0097237 IEA
    negative regulation of excitatory synapse assembly GO:1904890 IBA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    lamellipodium GO:0030027 IDA
    cell body GO:0044297 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified PTPN13 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for PTPN13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 OCLN 100506658
Proximity Label-MS Homo sapiens
3 KSR1  
Affinity Capture-MS Homo sapiens
4 RALGPS2 55103
Affinity Capture-MS Homo sapiens
5 MARCKS 4082
Proximity Label-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 PARD3 56288
Proximity Label-MS Homo sapiens
8 SRGAP2 23380
Affinity Capture-MS Homo sapiens
9 TBC1D8  
Affinity Capture-MS Homo sapiens
10 CBY1  
Affinity Capture-MS Homo sapiens
11 EIF4E2  
Affinity Capture-MS Homo sapiens
12 KIF13B 23303
Affinity Capture-MS Homo sapiens
13 MRFAP1L1  
Affinity Capture-MS Homo sapiens
14 MLLT4 4301
Proximity Label-MS Homo sapiens
15 HSPA2 3306
Affinity Capture-MS Homo sapiens
16 ZBTB21  
Affinity Capture-MS Homo sapiens
17 YWHAG 7532
Affinity Capture-MS Homo sapiens
18 C11orf52 91894
Proximity Label-MS Homo sapiens
19 STX6 10228
Proximity Label-MS Homo sapiens
20 YWHAH 7533
Affinity Capture-MS Homo sapiens
21 LRFN1  
Affinity Capture-MS Homo sapiens
22 MECP2 4204
Affinity Capture-MS Homo sapiens
23 RAB35 11021
Proximity Label-MS Homo sapiens
24 PKN2 5586
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 EBAG9 9166
Proximity Label-MS Homo sapiens
26 AMOT  
Proximity Label-MS Homo sapiens
27 METTL10  
Affinity Capture-MS Homo sapiens
28 YWHAB 7529
Affinity Capture-MS Homo sapiens
29 PLEKHA1 59338
Affinity Capture-MS Homo sapiens
30 S100A2 6273
Affinity Capture-MS Homo sapiens
31 FASLG 356
Reconstituted Complex Homo sapiens
32 MIB1 57534
Proximity Label-MS Homo sapiens
33 RGS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CTDP1 9150
Affinity Capture-MS Homo sapiens
35 WHSC1 7468
Affinity Capture-MS Homo sapiens
36 CXADR 1525
Proximity Label-MS Homo sapiens
37 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
38 LYN 4067
Proximity Label-MS Homo sapiens
39 MCAM 4162
Proximity Label-MS Homo sapiens
40 DNAJC5 80331
Proximity Label-MS Homo sapiens
41 YWHAE 7531
Affinity Capture-MS Homo sapiens
42 DIRAS3  
Proximity Label-MS Homo sapiens
43 CTNNB1 1499
Biochemical Activity Homo sapiens
44 ARRDC3 57561
Affinity Capture-MS Homo sapiens
45 DNM2 1785
Co-fractionation Homo sapiens
46 TRIM72  
Affinity Capture-MS Homo sapiens
47 RAPGEF1 2889
Affinity Capture-MS Homo sapiens
48 TANC2  
Affinity Capture-MS Homo sapiens
49 KCTD3  
Affinity Capture-MS Homo sapiens
50 YWHAZ 7534
Affinity Capture-MS Homo sapiens
51 EPB41L4A 64097
Proximity Label-MS Homo sapiens
52 LAMP1 3916
Proximity Label-MS Homo sapiens
53 NFKBIA  
Reconstituted Complex Homo sapiens
54 INPP5E 56623
Affinity Capture-MS Homo sapiens
55 CUL3 8452
Affinity Capture-MS Homo sapiens
56 PTEN 5728
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MTNR1A  
Two-hybrid Homo sapiens
58 LPIN3 64900
Affinity Capture-MS Homo sapiens
59 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
60 CDC25C  
Affinity Capture-MS Homo sapiens
61 ARHGAP29 9411
Reconstituted Complex Homo sapiens
62 RAPGEF2 9693
Affinity Capture-MS Homo sapiens
63 RPA3 6119
Proximity Label-MS Homo sapiens
64 RASSF10  
Affinity Capture-MS Homo sapiens
65 GIGYF1  
Affinity Capture-MS Homo sapiens
66 GOLGA1  
Proximity Label-MS Homo sapiens
67 HSPA1A 3303
Affinity Capture-MS Homo sapiens
68 CGN  
Affinity Capture-MS Homo sapiens
69 PRKY  
Affinity Capture-MS Homo sapiens
70 RHOB 388
Proximity Label-MS Homo sapiens
71 Apc  
Two-hybrid Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
72 FLOT1 10211
Proximity Label-MS Homo sapiens
73 DENND4C 55667
Affinity Capture-MS Homo sapiens
74 RNF43  
Proximity Label-MS Homo sapiens
75 SH3RF3  
Affinity Capture-MS Homo sapiens
76 GIGYF2 26058
Affinity Capture-MS Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 FBXW11  
Affinity Capture-MS Homo sapiens
79 PARP1 142
Proximity Label-MS Homo sapiens
80 RTKN 6242
Affinity Capture-MS Homo sapiens
81 DENND1A 57706
Affinity Capture-MS Homo sapiens
82 PPM1H  
Affinity Capture-MS Homo sapiens
83 LCK 3932
Proximity Label-MS Homo sapiens
84 EFNB1 1947
Reconstituted Complex Homo sapiens
85 LATS2 26524
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
86 DFFB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 STK26 51765
Affinity Capture-MS Homo sapiens
88 DDX58 23586
Affinity Capture-RNA Homo sapiens
89 LATS1  
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
90 NF1 4763
Affinity Capture-MS Homo sapiens
91 STXBP4 252983
Affinity Capture-MS Homo sapiens
92 RAB7A 7879
Proximity Label-MS Homo sapiens
93 ZCCHC10  
Affinity Capture-MS Homo sapiens
94 EPHA2 1969
Proximity Label-MS Homo sapiens
95 GHR  
Far Western Homo sapiens
96 KRAS 3845
Proximity Label-MS Homo sapiens
97 Ufl1  
Affinity Capture-MS Mus musculus
98 CCDC96  
Affinity Capture-MS Homo sapiens
99 MAST3  
Affinity Capture-MS Homo sapiens
100 CCT8L2  
Affinity Capture-MS Homo sapiens
101 CAV1 857
Proximity Label-MS Homo sapiens
102 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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