Gene description for PARD3
Gene name par-3 family cell polarity regulator
Gene symbol PARD3
Other names/aliases ASIP
Baz
PAR3
PAR3alpha
PARD-3
PARD3A
PPP1R118
SE2-5L16
SE2-5LT1
SE2-5T2
Species Homo sapiens
 Database cross references - PARD3
ExoCarta ExoCarta_56288
Vesiclepedia VP_56288
Entrez Gene 56288
HGNC 16051
MIM 606745
UniProt Q8TEW0  
 PARD3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for PARD3
Molecular Function
    protein kinase C binding GO:0005080 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 ISS
    phosphatidylinositol-3-phosphate binding GO:0032266 ISS
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISS
    protein binding GO:0005515 IPI
    protein phosphatase binding GO:0019903 IEA
Biological Process
    asymmetric cell division GO:0008356 TAS
    protein targeting to membrane GO:0006612 ISS
    wound healing, spreading of cells GO:0044319 IEA
    regulation of cellular localization GO:0060341 IEA
    apical constriction GO:0003383 IEA
    protein kinase C-activating G-protein coupled receptor signaling pathway GO:0007205 TAS
    regulation of actin filament-based process GO:0032970 IEA
    establishment of epithelial cell polarity GO:0090162 ISS
    cell junction assembly GO:0034329 TAS
    cell cycle GO:0007049 IEA
    myelination in peripheral nervous system GO:0022011 ISS
    axonogenesis GO:0007409 TAS
    microtubule cytoskeleton organization GO:0000226 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 TAS
    centrosome localization GO:0051642 IEA
    establishment or maintenance of cell polarity GO:0007163 TAS
    cell-cell junction organization GO:0045216 TAS
    bicellular tight junction assembly GO:0070830 TAS
    positive regulation of myelination GO:0031643 ISS
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 ISS
    protein complex assembly GO:0006461 TAS
Subcellular Localization
    endomembrane system GO:0012505 IEA
    cell-cell adherens junction GO:0005913 IEA
    neuronal cell body GO:0043025 IEA
    spindle GO:0005819 IEA
    protein complex GO:0043234 IEA
    cell cortex GO:0005938 IEA
    cell-cell junction GO:0005911 ISS
    bicellular tight junction GO:0005923 ISS
    apical part of cell GO:0045177 IEA
    cell junction GO:0030054 TAS
    internode region of axon GO:0033269 ISS
    plasma membrane GO:0005886 TAS
    axonal growth cone GO:0044295 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PARD3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 196
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 197
MISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PARD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SFN 2810
Affinity Capture-MS Homo sapiens
2 CDC42 998
Affinity Capture-MS Homo sapiens
3 F11R 50848
Reconstituted Complex Homo sapiens
4 JAM3 83700
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 YWHAG 7532
Affinity Capture-MS Homo sapiens
6 PARD6G 84552
Invivo Homo sapiens
Invitro Homo sapiens
7 PARD6A  
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
8 PARD6B 84612
Invivo Homo sapiens
Invitro Homo sapiens
9 JAM2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 PVRL3 25945
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 YWHAB 7529
Affinity Capture-MS Homo sapiens
12 ECT2 1894
Affinity Capture-Western Homo sapiens
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