Gene description for RASAL2
Gene name RAS protein activator like 2
Gene symbol RASAL2
Other names/aliases NGAP
Species Homo sapiens
 Database cross references - RASAL2
ExoCarta ExoCarta_9462
Vesiclepedia VP_9462
Entrez Gene 9462
HGNC 9874
MIM 606136
UniProt Q9UJF2  
 RASAL2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for RASAL2
Molecular Function
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
Biological Process
    response to dietary excess GO:0002021 IEA
    signal transduction GO:0007165 TAS
    response to glucose GO:0009749 IEA
    gene expression GO:0010467 IEA
    multicellular organism growth GO:0035264 IEA
    adipose tissue development GO:0060612 IEA
    regulation of protein activation cascade GO:2000257 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified RASAL2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RASAL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 HDAC4  
Affinity Capture-MS Homo sapiens
3 OCLN 100506658
Proximity Label-MS Homo sapiens
4 SHC1 6464
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 KSR1  
Affinity Capture-MS Homo sapiens
6 RALGPS2 55103
Affinity Capture-MS Homo sapiens
7 MARCKS 4082
Proximity Label-MS Homo sapiens
8 GJA1 2697
Proximity Label-MS Homo sapiens
9 ENTPD5 957
Co-fractionation Homo sapiens
10 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 HSPA12B 116835
Proximity Label-MS Homo sapiens
12 CDC25B 994
Affinity Capture-MS Homo sapiens
13 SRGAP2 23380
Affinity Capture-MS Homo sapiens
14 CAMSAP2  
Affinity Capture-MS Homo sapiens
15 CBY1  
Affinity Capture-MS Homo sapiens
16 HSPA1A 3303
Affinity Capture-MS Homo sapiens
17 EIF4E2  
Affinity Capture-MS Homo sapiens
18 KIF13B 23303
Affinity Capture-MS Homo sapiens
19 MLLT4 4301
Proximity Label-MS Homo sapiens
20 HSPA2 3306
Affinity Capture-MS Homo sapiens
21 ZBTB21  
Affinity Capture-MS Homo sapiens
22 ZNF638 27332
Affinity Capture-MS Homo sapiens
23 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 TIAM1  
Affinity Capture-MS Homo sapiens
25 PHLDB2 90102
Affinity Capture-MS Homo sapiens
26 USHBP1  
Two-hybrid Homo sapiens
27 STX6 10228
Proximity Label-MS Homo sapiens
28 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 LRFN1  
Affinity Capture-MS Homo sapiens
30 RAB35 11021
Proximity Label-MS Homo sapiens
31 EBAG9 9166
Proximity Label-MS Homo sapiens
32 CTAGE5 4253
Two-hybrid Homo sapiens
33 KIAA1804  
Affinity Capture-MS Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 AGAP1  
Affinity Capture-MS Homo sapiens
37 Mgp 17313
Affinity Capture-MS Mus musculus
38 CDK16 5127
Affinity Capture-MS Homo sapiens
39 MCC 4163
Affinity Capture-MS Homo sapiens
40 ANKRD34A  
Affinity Capture-MS Homo sapiens
41 USP21  
Affinity Capture-MS Homo sapiens
42 CXADR 1525
Proximity Label-MS Homo sapiens
43 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
44 TESK2  
Affinity Capture-MS Homo sapiens
45 LYN 4067
Proximity Label-MS Homo sapiens
46 MCAM 4162
Proximity Label-MS Homo sapiens
47 DNAJC5 80331
Proximity Label-MS Homo sapiens
48 Ankrd28  
Affinity Capture-MS Mus musculus
49 PARD3 56288
Proximity Label-MS Homo sapiens
50 DIRAS3  
Proximity Label-MS Homo sapiens
51 LPIN3 64900
Affinity Capture-MS Homo sapiens
52 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CSNK1E 1454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 FAM110B  
Affinity Capture-MS Homo sapiens
55 KCTD3  
Affinity Capture-MS Homo sapiens
56 YWHAE 7531
Affinity Capture-MS Homo sapiens
57 LIMA1 51474
Affinity Capture-MS Homo sapiens
58 DCLK1 9201
Affinity Capture-MS Homo sapiens
59 YWHAZ 7534
Affinity Capture-MS Homo sapiens
60 BAG2 9532
Affinity Capture-MS Homo sapiens
61 EPB41L4A 64097
Proximity Label-MS Homo sapiens
62 LAMP1 3916
Proximity Label-MS Homo sapiens
63 INPP5E 56623
Affinity Capture-MS Homo sapiens
64 UBC 7316
Reconstituted Complex Homo sapiens
65 DAB2IP 153090
Affinity Capture-MS Homo sapiens
66 NR3C1 2908
Proximity Label-MS Homo sapiens
67 ARF6 382
Proximity Label-MS Homo sapiens
68 NADK 65220
Affinity Capture-MS Homo sapiens
69 SNX27 81609
Affinity Capture-MS Homo sapiens
70 SYDE1 85360
Affinity Capture-MS Homo sapiens
71 Prkci  
Affinity Capture-MS Mus musculus
72 PRKACA 5566
Proximity Label-MS Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 GIGYF1  
Affinity Capture-MS Homo sapiens
75 RAB9A 9367
Proximity Label-MS Homo sapiens
76 Cep78  
Affinity Capture-MS Mus musculus
77 CGN  
Affinity Capture-MS Homo sapiens
78 RHOB 388
Proximity Label-MS Homo sapiens
79 FLOT1 10211
Proximity Label-MS Homo sapiens
80 DENND4C 55667
Affinity Capture-MS Homo sapiens
81 ANKS1B 56899
Affinity Capture-MS Homo sapiens
82 RNF43  
Proximity Label-MS Homo sapiens
83 GIGYF2 26058
Affinity Capture-MS Homo sapiens
84 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
85 LAMTOR1 55004
Proximity Label-MS Homo sapiens
86 FAM53C 51307
Affinity Capture-MS Homo sapiens
87 RTKN 6242
Affinity Capture-MS Homo sapiens
88 DENND1A 57706
Affinity Capture-MS Homo sapiens
89 PPM1H  
Affinity Capture-MS Homo sapiens
90 EZR 7430
Proximity Label-MS Homo sapiens
91 SRSF12  
Affinity Capture-MS Homo sapiens
92 LCK 3932
Proximity Label-MS Homo sapiens
93 LMAN1 3998
Proximity Label-MS Homo sapiens
94 LATS2 26524
Proximity Label-MS Homo sapiens
95 CDC25C  
Affinity Capture-MS Homo sapiens
96 SYNGAP1  
Affinity Capture-MS Homo sapiens
97 C11orf52 91894
Proximity Label-MS Homo sapiens
98 NF1 4763
Affinity Capture-MS Homo sapiens
99 TRIM37  
Proximity Label-MS Homo sapiens
100 RAB2A 5862
Proximity Label-MS Homo sapiens
101 RAB7A 7879
Proximity Label-MS Homo sapiens
102 RAB11A 8766
Proximity Label-MS Homo sapiens
103 CIT 11113
Affinity Capture-MS Homo sapiens
104 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
105 EPHA2 1969
Proximity Label-MS Homo sapiens
106 KRAS 3845
Proximity Label-MS Homo sapiens
107 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
108 NFKBIB  
Affinity Capture-MS Homo sapiens
109 KIF1C 10749
Affinity Capture-MS Homo sapiens
110 MAST3  
Affinity Capture-MS Homo sapiens
111 MAGI1  
Affinity Capture-MS Homo sapiens
112 CAV1 857
Proximity Label-MS Homo sapiens
113 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
114 LCP1 3936
Co-fractionation Homo sapiens
115 TRAF1 7185
Two-hybrid Homo sapiens
View the network image/svg+xml



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