Gene description for NADK
Gene name NAD kinase
Gene symbol NADK
Other names/aliases dJ283E3.1
Species Homo sapiens
 Database cross references - NADK
ExoCarta ExoCarta_65220
Vesiclepedia VP_65220
Entrez Gene 65220
HGNC 29831
MIM 611616
UniProt O95544  
 NADK identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NADK
Molecular Function
    NAD+ kinase activity GO:0003951 IBA
    NAD+ kinase activity GO:0003951 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    NADP biosynthetic process GO:0006741 IBA
    phosphorylation GO:0016310 NAS
    NAD metabolic process GO:0019674 IEA
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IEA
    ATP metabolic process GO:0046034 NAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NADK in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for NADK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 DENND4C 55667
Affinity Capture-MS Homo sapiens
4 LRFN1  
Affinity Capture-MS Homo sapiens
5 LIMA1 51474
Affinity Capture-MS Homo sapiens
6 HDAC4  
Affinity Capture-MS Homo sapiens
7 KIAA1804  
Affinity Capture-MS Homo sapiens
8 GIGYF2 26058
Affinity Capture-MS Homo sapiens
9 RBM42  
Affinity Capture-MS Homo sapiens
10 SDCBP 6386
Two-hybrid Homo sapiens
11 MAPKAP1 79109
Affinity Capture-MS Homo sapiens
12 FAM53C 51307
Affinity Capture-MS Homo sapiens
13 GRB2 2885
Two-hybrid Homo sapiens
14 EEF1G 1937
Two-hybrid Homo sapiens
15 KSR1  
Affinity Capture-MS Homo sapiens
16 RALGPS2 55103
Affinity Capture-MS Homo sapiens
17 EGFR 1956
Negative Genetic Homo sapiens
18 RTKN 6242
Affinity Capture-MS Homo sapiens
19 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 DENND1A 57706
Affinity Capture-MS Homo sapiens
21 AGAP1  
Affinity Capture-MS Homo sapiens
22 PPM1H  
Affinity Capture-MS Homo sapiens
23 YWHAE 7531
Affinity Capture-MS Homo sapiens
24 INPP5E 56623
Affinity Capture-MS Homo sapiens
25 CDK16 5127
Affinity Capture-MS Homo sapiens
26 CUL3 8452
Affinity Capture-MS Homo sapiens
27 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CDC25B 994
Affinity Capture-MS Homo sapiens
29 KIF13B 23303
Affinity Capture-MS Homo sapiens
30 SRGAP2 23380
Affinity Capture-MS Homo sapiens
31 ANKRD34A  
Affinity Capture-MS Homo sapiens
32 USP21  
Affinity Capture-MS Homo sapiens
33 CAMSAP2  
Affinity Capture-MS Homo sapiens
34 CBY1  
Affinity Capture-MS Homo sapiens
35 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
36 NUDT18  
Two-hybrid Homo sapiens
37 GAB2 9846
Affinity Capture-MS Homo sapiens
38 EIF4E2  
Affinity Capture-MS Homo sapiens
39 TESK2  
Affinity Capture-MS Homo sapiens
40 SYDE1 85360
Affinity Capture-MS Homo sapiens
41 BICD1 636
Proximity Label-MS Homo sapiens
42 NF1 4763
Affinity Capture-MS Homo sapiens
43 CDC25C  
Affinity Capture-MS Homo sapiens
44 KIF1C 10749
Affinity Capture-MS Homo sapiens
45 MOV10 4343
Affinity Capture-RNA Homo sapiens
46 FAM110A  
Affinity Capture-MS Homo sapiens
47 ZBTB21  
Affinity Capture-MS Homo sapiens
48 GIGYF1  
Affinity Capture-MS Homo sapiens
49 YWHAQ 10971
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
50 MAGI1  
Affinity Capture-MS Homo sapiens
51 RASAL2 9462
Affinity Capture-MS Homo sapiens
52 ZNF638 27332
Affinity Capture-MS Homo sapiens
53 LPIN3 64900
Affinity Capture-MS Homo sapiens
54 CGN  
Affinity Capture-MS Homo sapiens
55 GLRX3 10539
Two-hybrid Homo sapiens
56 GDI1 2664
Affinity Capture-MS Homo sapiens
57 MTMR1 8776
Affinity Capture-MS Homo sapiens
58 SERPINA10 51156
Affinity Capture-MS Homo sapiens
59 KRAS 3845
Negative Genetic Homo sapiens
60 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 FAM110B  
Affinity Capture-MS Homo sapiens
62 MAST3  
Affinity Capture-MS Homo sapiens
63 SIPA1L1 26037
Affinity Capture-MS Homo sapiens
64 TIAM1  
Affinity Capture-MS Homo sapiens
65 PHLDB2 90102
Affinity Capture-MS Homo sapiens
66 KCTD3  
Affinity Capture-MS Homo sapiens
67 SRSF12  
Affinity Capture-MS Homo sapiens
68 LNX1  
Two-hybrid Homo sapiens
69 DCLK1 9201
Affinity Capture-MS Homo sapiens
70 EP300 2033
Affinity Capture-MS Homo sapiens
71 NAV1 89796
Affinity Capture-MS Homo sapiens
72 SH3PXD2A 9644
Affinity Capture-MS Homo sapiens
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